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Citations for Promega :
501 - 550 of 5340 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Reverse transcription (RT) was performed with AMV-reverse transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Biophysics 2023Quote: ... Reverse transcription was performed with M-MLV RT (Promega, USA) according to the manufacturer protocol with the primer Pow-TBE-3’ ...
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bioRxiv - Genomics 2019Quote: ... PCR was performed with gene-specific primer pairs (Table S15) using a DNA polymerase kit (Promega). The PCR conditions were 95 °C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... PCR was run with genomic DNA that was prepared with Wizard Genomic DNA Purification Kits (Promega).
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bioRxiv - Genomics 2023Quote: ... The single-stranded DNA generated using this method was purified using PCR purification kit (A9285; Promega) and quantified using Nanodrop ...
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bioRxiv - Biophysics 2023Quote: ... and extracted each from the gel using a standard protocol from PCR purification kit (Promega, A9282). We then mixed the three fragments together in 10 µl in molar ratio 1:2:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... a PCR reaction using the PCR Master Mix (Promega) and the primers rplU_Fwd and rplU_Rev (Table 2 ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed using GoTaq PCR Master Mix (Promega) with primers mo37 and ag489 ...
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bioRxiv - Biophysics 2022Quote: ... Two PCR reactions using GoTaq G2 PCR mix (Promega) and a pUC19 template were set up ...
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bioRxiv - Molecular Biology 2021Quote: ROS production was detected using GloMax®-Multi+ Detection System (Promega) according to published protocols with minor modifications (Bisceglia et al. ...
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bioRxiv - Microbiology 2021Quote: Luciferase activities were determined using a GloMax Multi detection system (Promega) reader ...
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bioRxiv - Biophysics 2020Quote: ... This was used for luminescent detection using MTase-Glo assay (Promega) as above ...
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bioRxiv - Synthetic Biology 2022Quote: ... Then 20 µL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... Then 4 µL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... luminescence detection reagent (Bright-Glo™ Luciferase Assay System, Promega, USA) was added to each well following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Then 4 μL/well of Bright-Glo luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... For Nano-Glo HiBit Lytic Detection System (HiBit assay) (Promega, N3040) and CellTiter-Glo Luminescent Cell Viability Assay (CTG ...
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bioRxiv - Microbiology 2019Quote: ... in a GloMax®-Multi+ Detection System (Promega, Madison, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then 4 μL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then 20 μL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Then 50 μl/well of Bright-Glo luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... an equal volume of the luciferase lysing and detection buffer (Promega) was added to each well ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and a Dual Injector System for GloMax-Multi Detection System (Promega) luminometer were used to measure luciferase activity ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Nano-Glo® HiBiT Lytic Detection System (PROMEGA N3030) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μl of NADP/NADPH-Glo™ Detection Reagent (Promega, G9081) was added to each well and incubated for 30 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... and Nano-Glo HiBit Lytic Detection System (Promega Cat No. N3040) was used to measure relative luciferase units (RLU ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was quantified using a Glomax Multi Detection Plate Reader (Promega).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μL/well of One-Glo detection reagent (Promega, cat # E6120) was added to all microplate wells via Multidrop ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using T3 ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA used for the different PCRs were purified by using the Wizard® Genomics Kit (Promega).
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bioRxiv - Bioengineering 2021Quote: ... and digested vector was isolated using the Promega Wizard SV gel extraction and PCR cleanup kit (Promega). Products were then ligated overnight at 16°C following the NEB T4 DNA ligase protocol with 50 ng of pSLQ615 ...
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bioRxiv - Neuroscience 2021Quote: ... was purified using gel clean-up kit (Wizard® SV Gel and PCR Clean-Up, A9281, Promega) (Figure S4B) ...
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bioRxiv - Zoology 2021Quote: ... Those containing single bright bands were purified using Wizard SV Gel and PCR Clean-up Kit (Promega), and sequenced in both directions using PCR primers at Sequetech Inc ...
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bioRxiv - Immunology 2021Quote: Targeted re-sequencing by the Sanger method (Microsynth AG) was performed on kit purified PCR products (Promega) from chromosomal DNA or expression vector templates using pre-mixed sequencing primers listed Table S2.
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bioRxiv - Immunology 2021Quote: ... isotypes from patients were pooled at equal concentrations and concentrated using Wizard PCR Clean-up kits (Promega) according to manufacturer’s instructions with 30 μl of elute ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Bioengineering 2022Quote: ... The resulting double-stranded DNA fragments were purified using a PCR Clean-up Kit (Promega, Cat# A9282). The purified DNA was then cloned into the entry vectors in place of the respective SERF using Golden Gate techniques [14 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using SP6 RNA polymerase and the DIG-labelling kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplified fragments were run on an agarose gel and purified using the Wizard SV kit (Promega). The sequences were cloned into the pUASz1.0 vector (54 ...
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bioRxiv - Bioengineering 2024Quote: ... The digested product was gel-extracted using the Wizard SV Gel and PCR Clean-Up Kit (Promega). The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences19 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generatioplan of the 18S and E SgRNA standards have been outlined previously.20 Both PCR products were serially diluted to produce standard curves in the range of 5 × 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and E SgRNA standards have been outlined previously.20 Both PCR products were serially diluted to produce standard curves in the range of 5 × 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences19 ...
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bioRxiv - Genetics 2019Quote: ... Reverse-transcription (RT) was performed using the MMLV retro-transcriptase (Promega) from 500 ng of total RNA in a final volume of 60 µl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μl reverse transcription enzyme M-MLV-RT (Promega, USA). PCR was performed using each specific primer set in a total volume of 20 μl containing 10 pmol of each primer ...
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bioRxiv - Microbiology 2022Quote: ... treatment and cDNA was synthesized using M-MLV RT (Promega, USA). Gene specific primers used (Table 4) ...