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Citations for Promega :
301 - 350 of 5334 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Kinase detection solution (10 μL) (Promega) was added and incubated for 15 minutes at room temperature and luminescence was measured on a plate reader.
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bioRxiv - Cancer Biology 2020Quote: ... a GloMax-Multi Detection System (Promega) was used ...
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bioRxiv - Cell Biology 2023Quote: GloMax Multi detection System (Promega, USA);
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bioRxiv - Microbiology 2024Quote: ... on Glomax Multi-Detection System (Promega).
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Genetics 2021Quote: ... PCR were performed with the kit GoTaq® Flexi (Promega, USA) using 20 ng DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... PCR products were cloned using pGEM®-TA cloning kit (Promega) and sequenced with T7/SP6 universal primers ...
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bioRxiv - Developmental Biology 2020Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified with a purification kit (Promega, USA), and then sequenced by the dideoxy termination reaction using an AB3130 DNAxl sequencer ...
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bioRxiv - Immunology 2021Quote: ... before kit purification (SV Gel and PCR Clean up System, Promega) and ligation in presence of T4 ligase (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... Quenchbody PCR products were purified using Wizard Clean up kits (Promega; as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... This was cleaned up using a PCR cleanup kit (Promega, A9282). The biotin handle and cosmid-I95 DNA were both digested for 2 h at 37 °C with SpeI-HF (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
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bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from PCR mixture was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Cells were selected by detection of HiBit protein using Nano Glo HiBiT Lytic Detection System (N3030, Promega). pX330 was a kind gift from Dr ...
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bioRxiv - Developmental Biology 2021Quote: ... The detection of translated products was performed using Nano-Glo® HiBiT Lytic Detection System (Promega, #N3030) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Microbiology 2022Quote: ... viral RNA was quantified by RT-qPCR using the GoTaq Probe 1-Step RT-qPCR System (Promega) on a QuantStudio3 (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the luminescence detection assay was performed following all the manufacturer’s recommendations (Dual-Luciferase Reporter Assay Kit System E1910 (Promega).
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bioRxiv - Neuroscience 2022Quote: ... The ATP concentration was measured using an ENLITEN ATP Assay System with a bioluminescence detection kit (Promega, Madison, WI). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... we isolated individual clones and screened for expression using the C-terminal nanoluc fusion (Nanoluc Lytic Detection Kit, Promega), followed by additional characterization of SMO agonist-induced ciliary accumulation via microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by centrifugation and lysed with the Passive Lysis Buffer of the Dual-Luciferase detection kit (Promega). 10 µl cell extract was mixed with an equal volume of firefly luciferase assay substrate from the kit ...
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bioRxiv - Plant Biology 2020Quote: ... The overlapping PCR products were separated and purified by the Spin Column PCR Product Purification Kit (Wizard SV Gel and PCR Clean-Up System) following manufacturer’s instruction (Promega, WI). Then ...
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Genetics 2023Quote: ... RNA (1000 ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript RT kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Molecular Biology 2019Quote: Validation of hsa-miR-548ba and hsa-miR-7973 direct binding to target gene 3’UTR was performed by using Dual-Glo Luciferase assay system (Promega) according to the user manual ...
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bioRxiv - Cell Biology 2020Quote: The sequence containing three consecutive perfect matches for hsa-miR-145-5p was excised from pSG5-Luc-3xmiR-145 (11) and sub-cloned in pMirGlo (Promega) to generate pMiRGlo 3xmiR-145.
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase assays were performed by co-transfecting HEK-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids using FuGENE6 (E2691; Promega) and performing the Dual-Luciferase Reporter Assay System (E1910 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription PCR was performed with a Reverse Transcriptase kit (Promega A3803), real-time PCR was performed using Powerup SYBR Green PCR master mix (A25743 ...
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bioRxiv - Genetics 2019Quote: ... the three amplicons were purified with a PCR clean up kit (Promega). Purified amplicons were quantified with Qubit assays (Q33216 ...
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bioRxiv - Cell Biology 2020Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI) unless otherwise indicated.
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bioRxiv - Microbiology 2021Quote: ... using The Wizard® SV Gel and PCR Clean-Up Kit (Promega), and ligated to pEVS143 via Gibson Assembly ...
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bioRxiv - Biophysics 2021Quote: ... This was cleaned up using a PCR clean up kit (Promega, A9282). The Biotin-handle and Cosmid-I95 DNA were both digested for 2 hours at 37°C with SpeI-HF (NEB ...
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bioRxiv - Genomics 2020Quote: ... The MeDIP DNA was purified using the PCR cleanup kit (A1460, Promega).
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bioRxiv - Bioengineering 2021Quote: ... a volume of binding buffer from PCR clean-up kit (Promega, A9282) was added followed by 2 µL (300 ng ...
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bioRxiv - Bioengineering 2021Quote: ... Those were purified by wizard gel and PCR clean-up kit (Promega), achieving a final concentration of 200 ng/µL ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI).
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR fragments were cleaned using a gel extraction kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Released DNA fragments were purified by PCR product purification kit (Promega, A9282) and used to generate libraries ...
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bioRxiv - Biophysics 2023Quote: ... the handles were purified using a PCR clean up kit (A9282, Promega).
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bioRxiv - Bioengineering 2023Quote: ... PCR amplicons of ∼300 bp were amplified using a GoTaq kit (Promega), separated on a 2% agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were cleaned using a gel extraction kit (Promega) according to the manufacturer’s protocol and used for cloning using a Gibson assembly protocol ...
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bioRxiv - Cell Biology 2024Quote: ... dsRNAs were generated from PCR amplicons using a T7 RiboMAX kit (Promega). dsRNA against the bacterial kanamycine resistance gene (KAN ...
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bioRxiv - Microbiology 2023Quote: ... AePer50 reverse transcription (RT) and qPCR reactions were performed using GoTaq® 1-Step RT-qPCR System (Promega), with 10 µl of 2X qPCR Master Mix ...
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bioRxiv - Cancer Biology 2021Quote: ... Nano-Glo HiBiT Lytic Detection System (Promega) was used for the assay ...
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bioRxiv - Neuroscience 2022Quote: ... a 10 μL kinase detection reagent (Promega) was added and incubated for 1 h at 25°C to convert the generated ADP to ATP ...
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bioRxiv - Cell Biology 2019Quote: ... and the GloMax Multi Detection System (Promega). For microRNAs mimics transient expression assay ...
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bioRxiv - Genetics 2021Quote: ... on the GLOMAX Multi Detection system (Promega). All experiments were performed in at least three biological replicates in sets of 4 replicates.