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Citations for Promega :
101 - 150 of 3675 citations for Urea Nitrogen Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Cancer Biology 2019Quote: ... beads were washed two extra times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
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bioRxiv - Cancer Biology 2019Quote: ... washed two times in lysis buffer and three times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were further diluted to a final urea concentration of 2 M and proteins digested with trypsin (Promega) (1/100 ...
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bioRxiv - Biophysics 2019Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to 2M urea and were digested in-solution overnight using Trypsin (Promega Corporation, Madison, WI) as previously described to derive peptides (Mosley et al. ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were diluted to 1.5 M urea using 20 mM HEPES (pH 8.5) and digested at 30°C overnight with trypsin (Promega) at a ratio of 70:1 (w/w) ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were further diluted to 1 M urea using 50 mM HEPES (pH8) and trypsin (Promega, Madison, WI) was added at a ratio of 1:20 enzyme ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were solubilized with 10 μL of 8M urea and 10 μL of 1% Protease MAX (Promega) in 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... beads were suspended in digestion buffer (Tris 50 mM pH 7.5, urea 2 M, 1 mM DTT and 5 µg.µl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested overnight at 37ºC in 1.5 M urea and 66 mM ammonium bicarbonate with modified trypsin (Promega) at a 1:50 (M/M ...
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bioRxiv - Cancer Biology 2021Quote: ... Nano-Glo HiBiT Lytic Detection System (Promega) was used for the assay ...
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bioRxiv - Neuroscience 2022Quote: ... a 10 μL kinase detection reagent (Promega) was added and incubated for 1 h at 25°C to convert the generated ADP to ATP ...
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bioRxiv - Cell Biology 2019Quote: ... and the GloMax Multi Detection System (Promega). For microRNAs mimics transient expression assay ...
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bioRxiv - Genetics 2021Quote: ... on the GLOMAX Multi Detection system (Promega). All experiments were performed in at least three biological replicates in sets of 4 replicates.
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bioRxiv - Molecular Biology 2024Quote: ... and the Glomax Multi+ Detection System (Promega). All data were standardized for Renilla activity ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Neuroscience 2019Quote: ATP levels were assessed with luciferin-luciferase based assay (Lundin & Thore, 1975; McElroy & DeLuca, 1983) using a commercial ATP assay system with bioluminescence detection kit (Enliten, Promega). ATP was measured according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The luminescence signal was developed for 20 min after adding 40 µl kinase detection reagent (kit) and measured with a GloMaxMulti plate reader (Promega). The average of three experiments performed in duplicate and normalized to PI3KC3-C1 activity were determined ...
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bioRxiv - Microbiology 2021Quote: ... from 12-72 hpt and luciferase activity was measured using the Dual Luciferase reagent kit and GloMax multi detection system (Promega). Firefly luciferase readings from the TBEV replicons were normalized to the Renilla values (pTK-Ren ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assays using in situ cell death detection kit (Promega). All images were acquired using a Zeiss LSM710 confocal microscope and Zen software (Zeiss).
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bioRxiv - Molecular Biology 2022Quote: For Lytic endpoint measurements cells were seeded as mentioned above and NanoLuc abundance was determined via the Nano-Glo HiBit lytic detection kit (Promega) following manufacturers recommendations ...
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bioRxiv - Microbiology 2022Quote: ... All VLP volumes were calculated and normalized to 1.4 x 107 RLU using the Nano-Glo® HiBiT Lytic Detection System kit (Promega).
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bioRxiv - Microbiology 2022Quote: ... 48 h post transfection cells were harvested for western blotting or luminescence detection using a Firefly Luciferase Assay kit (Promega) and a Glomax luminometer (Promega) ...
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Molecular Biology 2021Quote: Muscles were pulverized in liquid nitrogen using a chilled mortar and pestle (Plattner’s) and re-suspended in passive lysis buffer (Promega). Luciferase results were obtained by reading 5 µL of sample with 50 µL luciferase assay reagents in a Glomax Luminometer according to manufacturer’s specifications.
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bioRxiv - Cancer Biology 2019Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Immunology 2021Quote: ... Beads were washed with urea buffer and trypsinized on-bead overnight at room temperature with 20 μg trypsin (Promega). The next day ...
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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bioRxiv - Systems Biology 2021Quote: ... Then urea concentration was diluted to 1.5 M with HPLC water and 1:50 (w/w) trypsin (Promega GmbH) was added for 12 h at 37 °C with mixing at 700 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Following the dilution of the sample with 100 mM ammonium bicarbonate to a final urea concentration of 800 mM the proteins were digested further with 0.2 µg/µl trypsin (Promega) (37 °C ...
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bioRxiv - Biophysics 2020Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were diluted to a final concentration of 2 M urea by addition of 100 mM NH4OAC (pH 6.8) and were digested overnight with sequencing-grade trypsin (Promega) at a 1:100 (enzyme:substrate ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were diluted with 100 mM ammonium bicarbonate to a final urea concentration of 1.5 M and followed by digestion using trypsin (Promega) (37 °C ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were trypsinised on the beads in 60 μl of Buffer I (2 M urea, 50 mM Tris-HCl pH 7.5, 5 μg/ml Trypsin [modified sequencing-grade trypsin; Promega]) for 30 min at 37°C in a thermomixer ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were pelleted at 20,000 × g and washed with ice cold 10 mM HCl / 90% acetone before resuspension in 60 μL 4 M Urea and 0.5 × ProteaseMax (Promega), vortexed ...
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bioRxiv - Immunology 2020Quote: ... Samples were then diluted with 50 mM HEPBS pH 8.3 to reduce urea concentration down to 1 M and digested with trypsin (Promega) at 1:10 enzyme:substrate ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... for 4 h at 37°C and then the urea concentration was reduced to 1.5 M and digested was finalized with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Precipitates were solubilised in 8M urea (20mM HEPES, pH 8) and digested with Lysine C (1:50 ratio; Promega) for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were diluted with 100 mM TEAB until containing 0.6 M urea and digested with sequencing grade modified trypsin (Promega) using a 1:150 enzyme-to-substrate ratio at 37°C for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein were digested overnight at 37°C in 2 or 1.5 M urea (for BB22OP and RIMD 221063 samples, respectively) and 25 mM ammonium bicarbonate with modified trypsin (Promega), in a 1:50 (M/M ...
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bioRxiv - Molecular Biology 2023Quote: ... and then diluted to 1M urea with 200 mM ABC for trypsin digestion (Sequence-grade, #V5111, Promega, WI, US) (1 µg ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg/μL sequencing-grade trypsin (Promega). Tryptic peptides were eluted ...