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Citations for Promega :
451 - 500 of 3876 citations for Urea Nitrogen Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... diluted 1:5000 in 5% milk in TBST were used for detection by the addition of an enhanced chemiluminescence (ECL) substrate (Promega). Western blot quantification was performed using Fiji software (Schindelin et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time PCR was performed on an ABI Prism 7900 detection system using Gotaq master mix (Promega, Madison, WI, USA). Expression relative to the housekeeping gene TBP was calculated using the delta(d)Ct method and expressed as 2^(-dCT ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl of lysate was transferred to a black 96-well Optiplate using 50 μl of Firefly and 50 μl of Renilla detection reagents (reagents were either from Promega or home-made ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 μl of diluted media added to a white 96 well plate (CLS3610) before the addition of 50 μl of Lactate/Glucose detection reagent (Promega). Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to a modified version of the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of HiBiT proteins in the concentrated EV fraction was measured using the Nano Glo HiBiT Lytic Detection System (Promega). EV marker proteins in the concentrated EV fraction were detected by western blotting as described above.
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated in the dark for 2 min and then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Cell Biology 2021Quote: The quantification of dead cells in lung tissue was performed using an in-situ apoptosis detection DeadEnd Colorimetric TUNEL System (Promega) as previously described (88) ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega).
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega). The resulting luminescence signals were normalized based on the cell viability that was measured using the PrestoBlue Cell Viability Reagent (Thermo).
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bioRxiv - Synthetic Biology 2021Quote: Absolute protein concentration of HiBiT-tagged proteins was measured using the Nano-Glo® HiBiT Extracellular Detection System N2420 (Promega). CFPS reactions were diluted 104-106 -fold using 1X PLB+PI buffer (generated by mixing 2 mL of 5x Passive Lysis Buffer E1941 (Promega ...
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bioRxiv - Microbiology 2021Quote: Luminescence measurements were performed using percoll-enriched infected erythrocytes in 384 well microplates using the Nano-Glo HiBiT extracellular detection system (Promega). Cells were resuspended at 0.2% hematocrit in culture medium diluted with two volumes of 200:1:50 buffer:LgBiT:Furimazine ...
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bioRxiv - Cancer Biology 2020Quote: ... Induction of ISRE-Luc reporter was evaluated by measuring Firefly and Renilla luciferase activity with dual-luciferase reporter assays on Glomax-Multi Detection System (Promega). The results were presented as relative light units (RLU) ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of ATP13A2 in microsomes was measured using a luminescence-based ADP detection assay (ADP Glo Max assay; Promega). Reactions were performed in a final volume of 5 µL containing 50 mM MOPS-KOH pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence of FITC-dextran in serum was diluted to 1:10 with PBS and measured on a Promega GloMax Multi Detection System (Promega) at 490 nm excitation and 520 nm emission wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration quantified using the 660 nm Protein Assay Reagent (Pierce) supplemented with 50 mM Ionic Detergent Compatible Reagent (Pierce) in a GloMax Multi Detection Plate Reader (Promega). After boiling in 1X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit polyclonal antibody was used followed by detection with a goat anti-rabbit secondary antibody conjugated to horseradish peroxidase and developed using a chemiluminescent substrate (Promega). Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10 ...
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bioRxiv - Molecular Biology 2024Quote: ... the amount of fluorescence of supernatant and solubilized cells was measured with a Glomax multi + detection system (Promega, WI, USA). The efflux capacity was calculated from the ratio of fluorescent intensity in the supernatant and the total fluorescent intensity from the supernatant and cell lysate ...
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bioRxiv - Immunology 2023Quote: ... single cell clones were first screened for insertion of an in-frame repair template using the Nano-Glo HiBit Lytic Detection System (Promega). HiBit-positive clones were then expanded and screened by anti-CD19 Western blot and genomic DNA PCR for genotyping.
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bioRxiv - Molecular Biology 2023Quote: The bioluminescence resonance energy transfer (BRET) ubiquitination assay was performed using the NanoBRET Nano-Glo Detection Systems (Standard) (N1661, Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... Protein yield was quantified by measuring luminescence with the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) and HiBiT Control Protein (N3010 ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Microbiology 2023Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for 10min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μg of the protein lysates were diluted to 50 μL using the M-PER buffer followed by complementation with LgBiT using Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and bacterial bioluminescence was measured in relative luminescence units (RLUs) every 24 h for up to seven days using a GloMax Multi Detection System (Promega) (35) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured with a GloMax®-Multi Detection system using the Dual-Glo® Luciferase Assay System (Promega). Results were analyzed using Excel (Microsoft ...
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bioRxiv - Genomics 2023Quote: ... Cells were harvested 24 hours after transfection and luciferase measured using Luciferase Assay System and the GloMax Multi-Detection System (Promega). For Verteporfin (VP ...
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bioRxiv - Microbiology 2024Quote: ... TC-tr minigenome rescue cells were lysed and processed using the Nano-Glo® HiBiT Lytic Detection System (Promega Corporation), according to the manufacturer recommendations ...
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bioRxiv - Plant Biology 2024Quote: The probe for detection of AFB2 secondary siRNAs was made by incorporation of α32P-dCTP in a Prime-a-Gene reaction from Promega. The reaction was carried out as described in the manual from the manufacturer only with substitution of dCTP from the kit with α32P-dCTP ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 24 h post-induction was pelleted and washed twice with PBS before measuring A600 and GFP fluorescence using the Glomax Multi JR Detection System (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... The expression of extracellular HiBiT-BNLN was normalized to the total intracellular pool of HiBiT-BNLN by lysing the cells and measuring luminescence with Nano-Glo HiBiT lytic detection system (Promega, N2422), following the protocol.
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bioRxiv - Cell Biology 2021Quote: ... Real time quantitative PCR was performed in a CFX 96 BioRad CFX 96 Real-Time PCR detection instrument with GoTaq qRT-PCR master mix (Promega, A6002). The sequence of the primers used in this study are ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL normalised extract were diluted to 30 μL in passive lysis buffer and mixed with 30 μL Nano-Glo® HiBiT Extracellular Detection Reagent (Promega) and luminescence was detected as above ...
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bioRxiv - Synthetic Biology 2021Quote: The effect of DMSO and methyl ester version compounds (compound 34–38) on cell viability was determined using a CellTiter-GloTM ATP detection system (Promega #7573). For testing DMSO tolerance ...
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bioRxiv - Genetics 2021Quote: ... The luciferin is then utilized in a firefly luciferase reaction to generate a bright luminescent signal determined as RLUs using a GloMax-Multi Detection System: Luminometer (Promega, USA). 2/ Colorimetric detection of senescence-associated β galactosidase following the manufacturer’s protocol (Cell Signaling #9860) ...
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bioRxiv - Cell Biology 2020Quote: ... The assay plates were then equilibrated to room temperature for 15 minutes before the addition of 25 µl of Nano-Glo HiBiT Lytic Detection System (Promega #N3030.) The plates were then shaken at 300 rpm for 2 minutes on an orbital shaker ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was quantified by detection of ATP content in the culture medium with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) by following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assessment of total cellular HiBiT-GCase protein abundance was performed using the Nano-Glo® HiBiT Lytic Detection System (Cat.#N3040, Promega), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... opaque 96-well plates and reagents were added according to the manufacturer’s protocols for the Nano-Glo® HiBiT Extracellular Detection System (Promega Corp.). After a 10-minute incubation step ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Microbiology 2023Quote: ... The amount of pseudoviruses prepared was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega,Cat# N3040) as previously described.1–5 ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µl supernatant was incubated with 10 µl of freshly prepared nucleotide detection reagent for UDP-Glo assays (Promega, Cat#: V6961) according to manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... The detection of intracellular ATP level was conducted by BacTiter-Glo™ Microbial Cell Viability Assay (Promega Corporation, Leiden, the Netherlands). ATP disodium salt (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... PCR positive clones were then expanded and tested for their response to pomalidomide treatment (positive control compound) by Nano-Glo® HiBiT Lytic Detection assay (Promega). The PCR products of 4 positive clones that responded to pomalidomide treatment were subjected to next generation sequencing to confirm the insertion of the HiBiT tag at the appropriate position of the HBG1 gene.
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR analysis was conducted using the Roche LightCycler® 96 or the Bio-Rad CFX384 Touch™ with GoTaq SYBR green fluorescence detection (Promega). All reactions were performed in duplicate for at least two biological replicates (n = 11-14 ...