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Citations for Promega :
201 - 250 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized with the M-MLV Reverse Transcriptase (Promega) using 1μg of total RNAs and Oligo(dT) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated using the GoScript Reverse Transcription System (Promega). RTqPCR analysis was conducted in 384-well format using the CFX 384 Real-Time system (BioRad ...
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bioRxiv - Immunology 2022Quote: ... cDNA was generated with MMLV reverse transcriptase (Promega, Madison, WI). Real-time PCR was performed using Radiant qPCR mastermix (Alkali Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA was made using Improm-II Reverse Transcription System (Promega), following the manufacturer′s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... We reverse transcribed cDNA using M-MLV Reverse Transcriptase (Promega) and random hexamer primers as described previously (44) ...
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bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit ...
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bioRxiv - Immunology 2021Quote: ... cDNA was prepared using Reverse Transcription Systems Kit (Promega, WI) or rAmp
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was obtained using MMLV H Minus Reverse Transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Immunology 2019Quote: ... cDNA was obtained using M-MLV H- reverse-transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA synthesis was achieved using M-MLV reverse transcriptase (Promega). PCR reactions were performed in a T-ADVANCED S96G (Biometra ...
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bioRxiv - Genetics 2020Quote: cDNA was synthesized using the GoScript Reverse Transcriptase kit (Promega) following the recommended protocol with random hexanucleotides (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... and cDNA was prepared with M-MLV Reverse Transcriptase (Promega). The transcript levels were quantified using Power SYBR® Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... and cDNA was synthesized with the Reverse transcriptase kit (Promega) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNAs were reverse transcribed into cDNAs (A5001, Promega, China), and PCR reactions were performed to identify transcripts from different Tmem138 alleles ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cDNA was synthesized using the GoScript Reverse Transcription system (Promega) using random primers and oligo(dT) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with cDNA using FuGene 6 (Promega, E2691) following manufacturer’s guidelines and a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized with a reverse-transcriptase kit (Promega). PCR amplification was performed in the presence of SYBR green in a 7500 Fast Realtime PCR System (ABI) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated with the GoScript Reverse Transcription kit (Promega) with 1μg RNA from each sample ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized using the GoScript Reverse Transcription System (Promega). Quantitative real-time PCR was conducted using primer sets for the indicated transcripts and iTaq Universal SYBR Green master mix (Bio-Rad).
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA was prepared following the GoScript Reverse Transcriptase system (Promega); qRT-PCR was done on a QuantStudio 6 real-time PCR machine (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Diluted cDNAs were mixed with GoTaq qPCR Master Mix (Promega) and analysed with Piko-Real 96 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthetized using GoScriptTM Reverse Transcription System (Promega, #A5001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA were amplified with the GoTaq qPCR Master Mix (Promega) and the fluorescence was measure on a QuantStudio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized with M-MLV reverse transcriptase Kit (Promega) and diluted 1/5 before further application ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was obtained using M-MLV H-reverse-transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The cDNA was sub-cloned into pGEMT easy vector (Promega), and Sanger sequencing was done to confirm the right insert ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Complementary cDNA was synthetized using M-MLV Reverse transcriptase (Promega). Messenger RNA (mRNA ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transfection System (Promega), using oligoDT primers ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following manufacturer‘s protocol ...
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bioRxiv - Immunology 2023Quote: ... followed by cDNA synthesis using GoScript Reverse transcription mix (Promega). The detection of RIG-I ...
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bioRxiv - Neuroscience 2023Quote: ... Probe sequences were amplified from cDNA using GoTaq Polymerase (Promega) and ligated into the pGEM®-T vector (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... full-length KILR cDNA was cloned into pGEM-T (Promega). All constructs were confirmed by Sanger sequencing at the Australian Genome Research Facility (AGRF) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained using an ImPROM II kit (Promega, A3800) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA was synthesized with GoScript Reverse Transcriptase (Promega A5001) according to manufacturer protocols ...
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bioRxiv - Biochemistry 2024Quote: ... then NanoLuc cDNA from pNL1.1.CMV[Nluc/CMV] (Promega; N1091) was inserted at Not1 + Sac1 (NLuc-FOXA1-FKHD ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:1 with nuclease-free water (Promega). Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad ...
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bioRxiv - Synthetic Biology 2019Quote: Two to three colonies of TOP10 transformed cells were selected to verify through PCR with GoTaq DNA Polymerase (Promega) if the plasmids they contained were the desired constructions ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were cloned (in each of the two allele versions) into the pmirGLO Dual-Luciferase miRNA target expression vector (Promega). Constructs were cloned using the XhoI and SdaI restriction sites ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were allowed to grow for two doubling times and proliferation was measured by CellTiter-Glo® (Promega G7571) or CyQuant (Invitrogen C3511 ...
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bioRxiv - Immunology 2019Quote: ... then washed three times in lysis buffer and two times in ammonium biocarbonate (50mM) before peptide digestion (V5280, Promega). Peptides were loaded onto a 3-cm self-packed C18 capillary pre-column (Reprosil 5 μM ...
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bioRxiv - Molecular Biology 2019Quote: ... The two fragments were then ligated using an overlap extension PCR reaction and ligated into pGEM/T-Easy (Promega). Positive clones were digested with AscI and SalI and the dropout was subsequently ligated into an AscI- and SalI-linearized pENTR-PpU6-sgRNA-NGG plasmid.
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Genomics 2023Quote: ... following the recommendations of the manufacturer’s capping protocol (https://international.neb.com/protocols/0001/01/01/capping-protocol-m2080) with two changes: 3.5 µl of RNAse inhibitors (RNasin Plus RNase Inhibitor, catalog num N2611, Promega) were added to the capping reaction to avoid RNAse degradation ...
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bioRxiv - Biochemistry 2023Quote: BromoTag cell lines were generated in HEK293 cells via simultaneous transfection of two vectors at a 4:1 reagent:DNA ratio with FuGENE 6 (Promega). The first vector was a pMK-RQ vector containing 500 bp homology arms on either side of either an eGFP-IRES-BromoTag or eGFP-IRES-HiBiT-BromoTag sequence for integration into MCM4 and BRD4 ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...