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Citations for Promega :
51 - 100 of 174 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... we isolated the sequence-paired sites from the native mouse and human Hes1 and Hes5 genes and cloned these fragments into the promoterless pGL3-Basic vector (Promega). Our synthetic promoters ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in native lysis buffer and luciferase activity was assayed using the Dual-Luciferase Reporter Assay (E1910, Promega) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Extracts were analyzed using the Dual-Luciferase Reporter Assay Kit (Promega). The ratio of Luciferase/Renilla was calculated and normalized to experimental control (promoter in absence of experimental plasmid).
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bioRxiv - Cancer Biology 2019Quote: ... and cell extracts were subjected to Steady-Glo luciferase assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... To extract RNA the SV Total RNA extraction KIT (Promega®) was used following the product specifications ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extracts were treated using RQ1 RNAse-free DNAse (Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were quantified using a Quantus® fluorometer (Promega, USA) and the degree of DNA fragmentation was assessed using a 4200 TapeStation system (Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: Wheat germ extract with micrococcal nuclease treatment was purchased from Promega (L4380). The translation reaction mixture for single-molecule detection was assembled according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Systems Biology 2022Quote: ... LC-MS performance was quality controlled with yeast protein extract digest (Promega). MS raw data was stored and managed by openBIS (v20.10.0 ...
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bioRxiv - Biochemistry 2023Quote: MS-Compatible Human Protein Extract Digest (K562) was purchased from Promega (V6951), MassPREP E ...
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bioRxiv - Biochemistry 2023Quote: ... A whole-cell protein extract of Saccharomyces cerevisiae (Promega, V7341, lot 434786) was deployed ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA (100 ng) was translated in a 50% wheat germ extract (WGE, Promega) for 90 mins at 25°C in a final volume of 20 μL ...
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bioRxiv - Microbiology 2022Quote: In vitro transcribed CrPV RNA was incubated in Sf-21 cell extract (Promega) in the presence of [35S]-methionine-cysteine for 2 hours at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... cell extracts were harvested and measured using the Dual-Luciferase assay kit (Promega). Each condition was performed in triplicate.
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bioRxiv - Evolutionary Biology 2020Quote: RNA extracts were subjected to RNA quantification using the RNA Quantifluor System (Promega) and their RNA profiles were assessed on an Agilent RNA 6000 Nano chip (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Plant Biology 2023Quote: ... we used in vitro translation system based on Wheat Germ Extract (Promega Corporation), which has been used for SCR validation as reported previously31 ...
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bioRxiv - Microbiology 2024Quote: ... Cleared protein extracts were incubated with 200 μl of MagneHis solution (Promega, USA) overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Any DNA in the RNA extracts was removed by a DNase treatment (Promega, USA). The purity of the RNA extracts was verified by spectroscopic light absorbance measurements at 230 nm ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Cell Biology 2020Quote: ... The protein extract was digested with trypsin (Catalog No. V5280, Promega, Madison, WI, USA) (trypsin ...
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bioRxiv - Biochemistry 2022Quote: ... we performed pulldowns from Xenopus egg extract with Halo-Magne beads (cat # G7281, Promega) coupled to N-terminally Halo-tagged versions of either human wildtype ...
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bioRxiv - Pathology 2020Quote: All DNA extracts were amplified using the Powerplex ESI/ESX 16 System (PP16, Promega).
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bioRxiv - Developmental Biology 2020Quote: ... Cell extracts and luciferase assays were performed following the protocol of the manufacturer (Promega). Mean luciferase activities and standard deviations were plotted as fold activation when compared with the empty expression plasmid.
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bioRxiv - Genomics 2021Quote: ... The cell extracts were prepared 24 hrs post transfection using Reporter Lysis Buffer (Promega) and assayed for Luciferase activity in EnSpire 2300 multimode plate reader (Perkin Elmer) ...
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bioRxiv - Plant Biology 2022Quote: ... PsiYAB11 protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega). The protein-bound beads were incubated with 50 ng of adaptor-ligated gDNA fragments on a rotator for 1 h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 50 μl of leaf extract was mixed with 50 μl luciferase substrate (Promega, E1501) in a white-bottomed 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total RNA extract (1 µg) was incubated with random primers (Promega, Madison, WI, USA) at 70°C for 5 min and subsequently cooled on ice ...
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bioRxiv - Neuroscience 2023Quote: Cells were harvested to extract RNA using ReliaPrep™ RNA Cell Miniprep System (Promega). CDNA was reverse-transcribed using PrimeScript™ RT reagent Kit (Takara) ...
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bioRxiv - Cell Biology 2020Quote: ... cell extracts were assayed for luciferase activity using the Dual-Luciferase Reporter Assay Systems (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2019Quote: ... Extracts were treated with the calf intestinal phosphatase (CIP) as indicated by the supplier (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts were tested by the dual luciferase assay according to the manufacturer’s instructions (Promega).
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bioRxiv - Microbiology 2022Quote: ... This DNA extract was then gel purified and quantified using a low-mass ladder (Promega). PCR was performed on ∼50 ng of DNA in a reaction mixture containing 1X Takara ExTaq PCR buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Plant Biology 2023Quote: Proteins were expressed using the TnT Coupled Wheat Germ Extract System with SP6 promoter (Promega). Protein expression was confirmed by Western Blot ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts of equal protein amounts were first subjected to proteolysis with trypsin (V5113, Promega) at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity was measured in cell extracts using a Dual-Luciferase Reporter Assay Kit (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Evolutionary Biology 2019Quote: Dual luciferase (LUC) assay extracts were prepared using the Dual Luciferase Reporter Assay System kit (Promega). Five mg of powdered tissue was mixed with 100 mL of the passive lysis buffer (PLB ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from egg extracts using a Wizard Genomic DNA purification kit (Promega, A1120). 0.5 μg of DNA were used in labeling reactions in end-label buffer (0.5 mM dNTPs ...
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bioRxiv - Biochemistry 2020Quote: ... Two control reactions were performed: one containing 8 units of HeLa nuclear extract provided by Promega as a positive control and the other containing no nuclear protein as a negative control ...
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bioRxiv - Plant Biology 2021Quote: ... Halo–GmRAV fusion protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega) following the manufacturer’s specifications for expression ...
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bioRxiv - Molecular Biology 2022Quote: Whole cell extracts from cultured cell lines were prepared using 1x GLO lysis buffer (Promega, E266A). One third volume of 4x Laemli buffer was added to one volume whole cell extract samples ...
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bioRxiv - Plant Biology 2021Quote: ... 2-3 μg of the purified RNA was in vitro translated using wheat germ extract (Promega) at 25 °C for 2 h as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... using a coupled transcription-translation system containing wheat germ extract (TNT kit; Promega, Cat no. L5030), according to the manufacturer protocol.
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bioRxiv - Cell Biology 2021Quote: Cell extracts were prepared and assayed for luciferase activity according to the manufacturer’s instructions (Promega, WI) using the GloMax Luminometer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The luciferase luminescence in the cellular extracts was measured with Dual-Luciferase Reporter Assay (Promega, #E1910) on the Modulus Microplate Reader (Turner BioSystems) ...
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bioRxiv - Microbiology 2023Quote: ... The protein extracts were digested by Lys-C endoproteinase and then by trypsin (Trypsin Gold, Promega). Peptides were vacuum-dried and resuspended in 100 µl of solvent A (0.1% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... using 20 uL of each extract into which 20 uL luciferase assay substrate was injected (Promega). β-galactosidase activity was measured by absorbance at 420 nm in the Tecan Genios plate reader using 30 uL of each extract mixed with 30 uL of 2x Assay Buffer (Promega).