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Citations for Promega :
251 - 300 of 1790 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... and transcribed with a T7 RiboMAX Express Large Scale RNA Production System (Promega). RNA was purified with RNA crush-n-soak and concentrated with phenol chloroform isolation.
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega), and purified using LiCl precipitation.
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bioRxiv - Neuroscience 2021Quote: ... Myc-tagged MiD49 and MiD51 were produced via in vitro transcription/translation (IVT) (Promega). For in vitro ubiquitination assays ...
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bioRxiv - Molecular Biology 2023Quote: ... by PCR and cloned into the Halo-tagged pFN21A vector (Promega, Madison, WI, USA). The AMPKγ1 mutant vector wherein Ser260 and Thr262 were replaced with alanine was created using parent-template backbone mouse AMPKγ1- full-length vector (# 15996 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 nmol of HALO-tagged UBDs were immobilized on 100 μl HALOLink resin (Promega) in 500 μl HALO-coupling buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and 75 μg of Rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18 to 24 h before odorant stimulation using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and 75 μg of rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18–24 h before odorant stimulation using Lipofectamine 2000 (11668019 ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... were pre-coated with recombinant luciferase (0.4μg/ml, Promega, #E1701) or spike protein (2μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Genetics 2021Quote: ... sgRNA synthesis was performed using a T7 RiboMax Large Scale RNA Production System (Promega) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... A riboprobe was transcribed from the template using T7 RNA polymerase (Promega, Madison, WI) incorporating digoxigenin (DIG ...
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bioRxiv - Plant Biology 2021Quote: Reactions were performed using the TNT® T7 Quick Coupled Transcription/Translation System (Promega) with 1 μg of vector (NPH3 or variants in pGADT7 ...
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bioRxiv - Genomics 2020Quote: ... dsRNA was synthesized using the T7 RiboMAX Express RNAi System (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro translation was done using the TNT T7 Reticolucyte Lysate System (Promega Corp.), in the presence of radioactive 35S-methionine ...
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bioRxiv - Evolutionary Biology 2022Quote: ... In vitro reverse transcription was performed using the RiboMax T7 system (Promega, Madison, WI), and the product was biotinylated using the Pierce RNA 3’ End Desthiobiotinylation Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RNA was obtained by using a T7 RiboMAX Large Scale RNA Production System (Promega) and infectious viruses were generated by transfecting RNA into respective cells with the TransIT-mRNA Transfection Kit (Mirus) ...
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bioRxiv - Plant Biology 2022Quote: ... A digoxigenin (DIG)-labeled antisense (AS) probe was synthesized using the T7 polymerase (PROMEGA), for hybridization on pre-parasitic stage 2 juvenile nematodes (ppJ2) ...
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bioRxiv - Microbiology 2023Quote: ... using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Cat. No. P1320) with 1µg DNA template input ...
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bioRxiv - Microbiology 2024Quote: ... RNA was synthesised using the RiboMaX T7 express large-scale RNA production system (Promega). Samples were purified through an RNA clean and concentrator column (Zymo research ...
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bioRxiv - Molecular Biology 2024Quote: Biotinylated RNA probes were produced by in vitro transcription using T7 RNA polymerase (Promega), and by adding 1:10 CTP-biotin analog:CTP to the reaction mix ...
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bioRxiv - Biochemistry 2021Quote: ... The 3xFlag-m-PKD1-2xMyc/His insert was subcloned into pCI vector (Promega). To produce CTF expression constructs of human (h ...
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bioRxiv - Molecular Biology 2020Quote: A Myc-tagged construct was synthesized by Genewiz and cloned into the psiCHECK2 vector (Promega), replacing the synthetic Renilla Luciferase gene ...
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bioRxiv - Biochemistry 2022Quote: ... with or without RFP-tagged full-length Caskin1 using ViaFect Transfection Reagent (Promega, Madison, WI) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Myc-tagged DHX36 was constructed by inserting the ORF of DHX36 into pCMV-Myc (Promega). pHAGE-WT and -D126A DDX21 were constructed by inserting the flag-tagged WT and D126A DDX21 into pHAGE-bsd ...
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bioRxiv - Biochemistry 2022Quote: ... The preparation of Halo-tagged NEMO and its covalent attachment to Halo-Link Resin (Promega) have been described (Emmerich et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... purified Halo-tagged MultiDsk (15 μg per condition) was pre-incubated with HaloLink resin (Promega) for 1 h with rotation at room temperature in binding buffer (100 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we implemented a cell-free assay with purified recombinant luciferase (Promega) and luciferin (BrightGlo Luciferase Assay System ...
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bioRxiv - Bioengineering 2020Quote: ... The standard curve was prepared by diluting recombinant ATP (Promega, P1132). For both assays ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7 and digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant bacmids were transfected into Sf21 cells using Fugene6 (Promega E2693). Supernatant containing viruses (V0 ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease Inhibitor (1 uL/ml, Promega, Cat#N2511). The components of the high salt wash buffer are as follows ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant baculovirus was generated using FuGENE HD Transfection reagent (Promega) in Sf9 cells using Insect X-press (Lonza ...
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bioRxiv - Developmental Biology 2021Quote: ... and EGFP were synthesized using the RiboMAX Large Scale RNA Production System T7 kit (Promega). Approximately 100 μg of synthesized dsAntp was injected into the second chest spiracle at the first day of Bombyx larval wandering stage ...
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bioRxiv - Microbiology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... BlaR and GCaMP3 genes were cloned from pFN19K HaloTag T7 SP6 (Promega, Fitchburg, WI, USA), pYES6/CT (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNAs were in vitro transcribed (T7 RiboMAX Express Large Scale RNA Production System, Promega, USA) from the Nluc PCR template ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNAs were transcribed in vitro (T7 RiboMAX Express Large Scale RNA Production System, Promega, USA) from the linear fragment of the pET28-MVHL-UAA plasmid containing the T7 promoter ...
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bioRxiv - Cell Biology 2022Quote: ... used for dRNF146 RNAi were transcribed in vitro using T7 RNA polymerase (Promega, Cat #P2075) at 37°C for 4 hrs from the PCR products.
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Linearised plasmids were used as template for in vitro transcription using T7 RNA polymerase (Promega) and a ribonucleotide set (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Linearised plasmids were used as template for in vitro transcription using T7 RNA polymerase (Promega) and biotin RNA labeling mix (Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... was substituted to the Halo7 sequence from the FN19K HaloTag T7 SP6 Flexi Vector (Promega).
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bioRxiv - Evolutionary Biology 2022Quote: ... Double-stranded RNAs (dsRNAs) were in vitro synthesised using the RiboMax T7 System Kit (Promega). As a control we prepared GFP dsRNA from a pGreen Lantern plasmid (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... DNA templates were in vitro transcribed using T7 RiboMax Large Scale RNA Production Kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... RNA probes were labeled by in vitro transcription of plasmids with T7 RNA polymerase (Promega) in the presence of Biotin RNA labeling mix (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The irrelevant control gene luciferase (dsLuc) was amplified from the luciferase T7 control plasmid (Promega). Female mosquitoes were injected intrathoracically (Ramirez et al. ...
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bioRxiv - Immunology 2020Quote: In vitro transcribed (IVT) mRNA was synthesized using RiboMAX T7 Kit (Promega, Madison, WI, USA) as described (37 ...