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Citations for Promega :
101 - 150 of 1790 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Biochemistry 2023Quote: ... Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega, cat. no. L4140) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant (rTdT) enzyme (Promega). Cells were then incubated at 37 °C for 60 min ...
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bioRxiv - Cell Biology 2021Quote: ... linearised plasmid using T7 RNA polymerase (Promega). In vitro translation (Poet et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 1x RiboMAX Express T7 Buffer (Promega, P1320), and 2 μL T7 Express Enzyme Mix (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and 80 U T7 RNA polymerase (Promega). After 2 h incubation at 37°C ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: T7 RiboMAX Express RNAi System kit (Promega) was used to generate dsRNA-LvSTING dsRNA-LvcGAS and dsRNA-GFP (for controls ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Biochemistry 2021Quote: ... yeast (Saccharomyces cerevisiae) cell protein extract (Cat# V7341, V7461), and human K562 cell protein extract (Cat# V6951, V6941) were purchased from Promega Inc (WI ...
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bioRxiv - Microbiology 2022Quote: The HEV replicon plasmids were linearised with BglII before being used to generate T7 in vitro transcribed RNA using the HiScribe T7 ARCA mRNA kit with tailing following manufacturer’s instructions (Promega). RNA was purified using an RNA clean and concentrate kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: pSK-E2-nLuc replicon plasmid was linearised with BglII and pUC-HEV83-2 replicon plasmid was linearised with HindIII before being used to generate T7 in vitro transcribed RNA using the HiScribe T7 ARCA mRNA kit with tailing following manufacturer’s instructions (Promega). RNA was purified using an RNA clean and concentrate kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... the HaloTag-tagged SUV420H2 expression vector (FHC26822; Promega) was used as a template for PCR amplification of the target regions ...
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bioRxiv - Cell Biology 2023Quote: The HaloTag-tagged KDM4D expression vector (FHC06842; Promega), the PB533-based H2B-Halo expression vector (Uchino et al. ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro transcribed and translated FLAG-tagged ERRα protein was made using the TnT reticulocyte reaction (Promega cat #L1170, 2h on 37°C) or the TnT wheat germ extract (cat #L5030 ...
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bioRxiv - Cell Biology 2022Quote: ... in pcDNA3/V5-Dest40 vector were expressed in T7 rabbit reticulocyte lysates using TNT T7 Quick Coupled transcription/Transaltion System (Cat# TM045, Promega) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Five µg of purified DNA template were used for T7 in vitro transcription using RiboMAX large-scale RNA production system T7 (Promega) in presence of 40 mM cap analog (Ribo m7G Cap ...
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bioRxiv - Neuroscience 2020Quote: ... 1U/uL recombinant RNAsin(Promega), 10mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RNasin Ribonuclease inhibitor (Promega), and SuperScript II reverse transcriptase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and recombinant RNasin inhibitor (Promega). The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... and ribonuclease (RNasin Recombinant, Promega) at concentrations of 1:1000 (sucrose buffer ...
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bioRxiv - Neuroscience 2021Quote: ... using the T7 or SP6 Riboprobe kit (Promega). 20 μm sections from fixed tissue were labeled and detected as previously described3 ...
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bioRxiv - Microbiology 2021Quote: The T7 RiboMAX Express RNAi System (Promega, P1700) was used to synthesize double-strand RNA according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase (Promega; Cat. No. P2077). Sample preparation ...
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bioRxiv - Molecular Biology 2022Quote: ... the T7 RiboMAXTM Express RNAi System (Promega, USA) was used following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: The T7 RiboMAX Express RNAi System (Promega, P1700) was used to synthesize double stranded RNA (dsRNA ...
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bioRxiv - Systems Biology 2024Quote: ... 2.5 μL of Express Mix (T7 RiboMAX; Promega), and an appropriate volume of nuclease-free water to achieve a final reaction volume of up to 25 μL ...
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bioRxiv - Developmental Biology 2024Quote: ... R-TAATACGACTCACTATAGGGAGCTGCACGCGACCATCT and transcribed using T7 polymerase (Promega). Categorical scoring was completed blinded.
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... probe was generated by an in vitro transcription system using primer pairs with T7 and SP6 promoter regions (T7 and SP6 Ribomax™ large scale RNA kit, Promega) (Mlig-pro46 ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... were cloned into pcDNA3.1-myc-His plasmid (Promega). For sequencing we used a sequence analyser (ABI Prism 3100 Avant ...
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bioRxiv - Microbiology 2023Quote: ... or the Magne-His purification system (Promega Corporation) for PlzARD-RD per manufacturer protocol ...
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bioRxiv - Genetics 2021Quote: ... Colonies were genotyped using the T7/SP6 primers (Promega). Plasmids from the validated colonies were isolated using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Neuroscience 2019Quote: ... it was transcribed with T7 polymerase (Promega, Madison, WI) in the presence of digoxigenin (DIG)-conjugated UTP (Roche Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... using T7 or SP6 RNA polymerase (Promega, Madison, WI). All antisense riboprobes revealed distinctive signal distributions that were consistent with previous studies (Du et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 μL T7 Express Enzyme Mix (Promega, P1320) and incubated at 37 °C for 4 hours unless otherwise indicated ...
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bioRxiv - Cell Biology 2019Quote: ... Luciferase expression vector under T7 promoter was from Promega. Human HSP70 under T7 promoter was cloned into pCH16 vector (a kind gift of H.-C ...
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bioRxiv - Microbiology 2021Quote: ... In vitro transcription reactions (Promega MegaScript T7 transcription kit) containing ∼400 ng of the purified PCR product ...
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bioRxiv - Neuroscience 2020Quote: ... and polymerase T7 (Promega, Fisher Scientific, St-Laurent, QC). Labelled brain slices were placed against Kodak Biomax MR X-ray film (VWR ...
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bioRxiv - Developmental Biology 2023Quote: ... using the T7/T3 Riboprobe Combination System kit (Promega) and the Digoxigenin labeling kit (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... and transcription with T7 RNA polymerase (Promega, Madison, WI).
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bioRxiv - Systems Biology 2024Quote: ... 12.5 μL of 2x Express Buffer (T7 RiboMAX; Promega), 2.5 μL of Express Mix (T7 RiboMAX ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.625 μl of recombinant RNasin (Promega), 1 μl of random hexamer primers (Promega ...
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bioRxiv - Physiology 2019Quote: ... 100 U/mL recombinant RNasin (Promega), 100 μg/mL cycloheximide ...