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Citations for Promega :
201 - 250 of 1313 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Proteins were then digested with trypsin (Promega) overnight at 37°C with an enzyme trypsin ratio of 1:50 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein digestion was done with trypsin (Promega) at a 1:50 ratio and incubated for 4 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested with trypsin (Promega #V5111) in 200mM HEPES buffer pH 8.5 with 2% acetonitrile (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested with either Trypsin (Promega), a combination of Trypsin and GluC (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested with trypsin (V5280, Promega) overnight ...
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bioRxiv - Microbiology 2021Quote: ... For protein digestion trypsin (Promega, Walldorf, Germany) was added in an enzyme to substrate ratio of 1:100 before incubation at 37 °C for 14 h ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were first digested with trypsin (Promega) for 1 h at 37°C (sample to enzyme ratio 1:20 ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were proteolytically digested with trypsin (Promega), chymotrypsin (Promega) ...
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bioRxiv - Immunology 2021Quote: ... Proteins were digested with trypsin (Promega®) following filter-aided sample preparation (FASP ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with trypsin (V5111, Promega). Peptide concentrations were determined using PierceTM BCA Protein Assay Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested with LysC (Promega #VA1170) at 1:30 (enzyme:protein ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then digested with trypsin (Promega) overnight at 37°C with an enzyme trypsin ratio of 1:50 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were digested using trypsin (V5111, Promega) at a 1:20 (enzyme to protein ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then digested with trypsin (Promega) - 1:100 enzyme-to- protein ratio at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... G protein chimera (Gsx) and Glo22F (Promega) using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested using Trypsin Gold (Promega) in 100 mM HEPES pH 7.6 at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Denatured proteins were digested with chymotrypsin (Promega) at 1:100 (enzyme:substrate(w:w) ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with Trypsin (Promega, UK) overnight at 37 °C at an enzyme to protein ratio of 1:20 ...
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bioRxiv - Plant Biology 2023Quote: ... and tryptic digestion of proteins (V5111; Promega, Madison ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were pre-digested with LysC (Promega) for 2 h at 25 °C and digested with trypsin (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... or the MagneHis Protein Purification System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Subsequently proteins were digested with trypsin (Promega) at 37°C for 4 h ...
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bioRxiv - Biochemistry 2023Quote: ... and the proteins were tryptic digested (Promega) at 37°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested with trypsin gold (Promega) at 1:50 enzyme to protein ratio in 50 mM TEAB ...
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bioRxiv - Systems Biology 2019Quote: ... 1% Triton® X-100 0.1% Na deoxycholate) supplemented with protease inhibitor cocktail (Promega, G6521). Cells were lysed by mechanically means by passaging of cells through 26-gauge needle 5 times ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were splited After that cells were lysed with 1 X Passive Lysis Buffer (Promega). Renilla (RL ...
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bioRxiv - Microbiology 2021Quote: ... 4°C) and then resuspended in 100 µl 1 x Passive Lysis Buffer (PLB, Promega). 45 µl of the luciferase assay substrate dissolved in Luciferase Assay Buffer II was plated on a 96-well plate and 10 µl of the cell lysate was added immediately before measurement of Fluc activity using a Tecan reader ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibody was incubated in 1× PBST (PBS + 0.1% Triton X-100) + 0.2% BSA (Promega) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative PCR (qPCR) reactions included 10 μl 1 X GoTaq® qPCR Master Mix (Promega), 0.5 μM of each primer and 5 μl template cDNA ...
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bioRxiv - Physiology 2023Quote: ... 0.5% Triton X-100 (with added protease inhibitors; Promega, Cat. No. G6521; Madison, WI, USA). Samples were then centrifuged at 1500 g for 10 min at 4°C and the soluble/sarcoplasmic fraction supernatants were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% Triton X-100) with the addition 15 ng of Lambda DNA (Promega, Fitchburg, WI) in 20 µL total reaction volume for 3 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The interactions between host protein and RHDV nonstructural proteins were evaluated using a CheckMate Mammalian Two-Hybrid System (Promega). The proteins expressed from the pACT vector recombinant plasmid acted as prey proteins ...
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bioRxiv - Microbiology 2021Quote: ... Pull down of Halo-PfSR1 interacting proteins was performed using the HaloTag® Protein Purification System (Promega, cat # G1913) according to the manufacturer guidelines ...
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bioRxiv - Microbiology 2022Quote: ... the Halo-BysR protein was expressed using the TNT SP6 Wheat Germ Protein Expression System (Promega, Fitchburg, WI, USA). The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... the PhDEF and PhGLO1 proteins were produced with the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were expressed using TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) with 2 µg Plasmid DNA as input ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Cell Biology 2019Quote: The primary antibody was incubated in 1× PBST (PBS + 0.1% Triton X-100) + 0.2% BSA (Promega) overnight at 4°C ...
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bioRxiv - Bioengineering 2021Quote: A mNeonGreen construct was transfected into HEK293T-X cells with FuGENE HD Transfection Reagent (E2311; Promega). The cells were then selected with puromycin (NC9138068 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed once with PBS and lysed in 40μl of 1 x CCLR buffer (Promega) for 10 min on a rocking plate at RT ...
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bioRxiv - Biochemistry 2022Quote: ... Product length was determined with respect to ladder product sizes of Lambda x HindIII DNA (Promega). The front (fastest migrating population ...
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bioRxiv - Plant Biology 2023Quote: ... DNA fragments were excised from the gel by means of x-tracta™ Gel Extractor (Promega) and purified using Wizard® SV Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... proteins were expressed by in vitro transcription/translation using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) prior to incubation with the affinity resins ...
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bioRxiv - Biochemistry 2020Quote: The two Munc13-Halo proteins were labeled by incubating the protein with Alexa 488 conjugated with HaloTag® from Promega, as described before (2) ...
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bioRxiv - Synthetic Biology 2021Quote: Absolute protein concentration of HiBiT-tagged proteins was measured using the Nano-Glo® HiBiT Extracellular Detection System N2420 (Promega). CFPS reactions were diluted 104-106 -fold using 1X PLB+PI buffer (generated by mixing 2 mL of 5x Passive Lysis Buffer E1941 (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... yeast (Saccharomyces cerevisiae) cell protein extract (Cat# V7341, V7461), and human K562 cell protein extract (Cat# V6951, V6941) were purchased from Promega Inc (WI ...
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bioRxiv - Plant Biology 2022Quote: ... EMF2 and POT1a proteins) or a myc-tag (pGBKT7-DEST; TRB proteins) using a TNT Quick Coupled Transcription/Translation system (Promega) in 50 μl reaction volumes according to the manufacturer’s instructions ...