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Citations for Promega :
151 - 200 of 1313 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Molecular Biology 2019Quote: ... The recombinant plasmids were prepared from some positive clones using the PureYield Plasmid Miniprep System (Cat #A1222, Promega, Madison, USA). The sequencing of the cloned insert was carried out according to Sanger et al ...
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bioRxiv - Biochemistry 2019Quote: The 5’UTRs of each transcript variant were transcribed and purified in vitro as previously described46 from linearized recombinant psiCHECK2 vector (Promega) that contained one of the three UTR sequences downstream of a T7 promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from pooled neurons that were stored at −80 in saline solution with 1% (v/v) Recombinant RNasin Ribonuclease Inhibitor (Promega). The RNA was extracted with the Quick-RNA micro prep kit (Zymo ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with 8xTEAD synthetic YAP/TAZ-responsive promoter-luciferase reporter and treated with BSA or recombinant TNC (5 µg/mL) for 48 h to measure YAP1 signaling activity using Dual-luciferase Reporter Assay Kit (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Cell Biology 2022Quote: ... 60,000 nuclei were sorted using an SH800 sorter (Sony) into 50 μl of collection buffer [1.0U/μL Recombinant RNAsin (Promega, PAN2515), 5% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 100 ng of either XeARPP19 or ClyARPP19 were incubated with 200 μM ATP and 25 units of recombinant bovine PKA (Promega) in PKA Buffer for 3 hours at 37°C under 1200 RPM stirring ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Neuroscience 2023Quote: Nine DIV hippocampal slice cultures were randomly assigned to treatment groups with the following drugs dissolved in serum-free medium: recombinant human BDNF (250 ng/mL, Promega); BDNF + K-252a (200 nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant methylated BCAR3 (see methylation assay method) was sliced from the gel and digested with modified trypsin (sequencing grade, Promega). The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Cancer Biology 2021Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1X sample buffer (4X stock ...
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bioRxiv - Cancer Biology 2022Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1 X sample buffer (4 X stock ...
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bioRxiv - Cell Biology 2022Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1X sample buffer (4X stock ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were co-expressed using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein G magnetic beads (Promega; G7471) were washed with PBS buffer and then added to the supernatant in a 1:10 volumetric ratio ...
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bioRxiv - Microbiology 2021Quote: ... Protein was digested by trypsin (Promega) at a ratio of 1:50 (trypsin:protein ...
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bioRxiv - Biophysics 2020Quote: ... Fusion Protein were purchased from Promega. Nickel superflow resin was purchased from Qiagen (Hilden ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested with trypsin (Promega) in 50 mM ABC overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested with trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin (Promega) to be peptides and then processed according to the manufacturer’s protocol for TMT kit ...
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bioRxiv - Plant Biology 2023Quote: ... and HiBiT Control Protein (N3010, Promega). We were unable to obtain soluble protein of AtARF9 ...
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bioRxiv - Zoology 2023Quote: ... Proteins were digested with trypsin (Promega Porcine trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested by trypsin (Promega) over night at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Microbiology 2019Quote: ... The cells were permeabilised by adding 20 µl of 0.2% Triton X-100 (Promega) in 1x PBS for 10 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: 4 x 105 HEK293T cells were lysed in 100 μl Passive Lysis Buffer (Promega). 10 μl of cell lysates were boiled with 2.5 μl 4x gel loading buffer for 5 min at 98°C and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel for 45 min at 200 V ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Biophysics 2023Quote: ... The initial recombinant baculovirus was generated by transfecting adherent Sf9 cells with the bacmid DNA using the FuGENE HD reagent (Promega, USA) and used to infect suspension cells for virus amplification ...
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bioRxiv - Developmental Biology 2024Quote: ... The katna1 digoxigenin-labelled sense and antisense riboprobes were synthetized from the linearised recombinant TOPO® TA cloning vector using T7 and T3 RNA Polymerase (Promega) according to the supplier’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The ANAC50 protein was purified with the HaloTag® Protein Purification System (Promega, Madison, Wisconsin, United States) using 20 μL Expression reaction for each EMSA reaction. ...
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bioRxiv - Cancer Biology 2021Quote: ... 1500 μg total protein lysate was 100ul slurry of pre-cleared with Protein G agarose beads (Promega), followed by overnight incubation with AXL antibody (2ug Ab/ 100 ul lysate) ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The recombinant plasmid was digested with Sac1 and Nde1 restriction enzyme and sub-cloned in pGL3 Basic luciferase vector (Promega, Madison, USA).
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were digested with trypsin (Promega Corporation) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with 1.5μg Trypsin (Promega) at 37°C for 12 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested overnight with trypsin (Promega) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were first digested with chymotrypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested overnight with trypsin (Promega). The resulting peptides were labelled with the TMT sixplex isobaric mass tag ...
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bioRxiv - Systems Biology 2020Quote: ... Proteins were then digested with trypsin (Promega) on-beads as previously detailed (66) ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were digested overnight using trypsin (Promega, Sequencing Grade Modified Trypsin ...