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Citations for Promega :
1 - 50 of 5331 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and Glutamine/Glutamate-Glo (Glutamine and Glutamate) kits from Promega according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Instructions from Glutamine/Glutamate-Glo Kit (J8021, Promega) were followed past this point.
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bioRxiv - Microbiology 2024Quote: ... 500 IU/ml of human interferon-gamma (IFN-γ) for the gamma activated sequence reporter for 18h and lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and assayed for firefly and Renilla luciferase activities using the Dual luciferase reporter assay system (Promega E1960 ...
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bioRxiv - Immunology 2022Quote: ... Glutamine in culture supernatant was determined using the Glutamine/Glutamate-Glo (TM) Assay kit (Promega, Madison, WI) and used for calculating the kinetics of glutamine uptake/utilization ...
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bioRxiv - Bioengineering 2022Quote: ... Glutamine consumption (Glutamine/Glutamate-Glo, J8021, Promega), and Glutamate production (Glutamate-Glo ...
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bioRxiv - Cell Biology 2024Quote: ... Radiolabeled rat ornithine transcarbamylase (OTC) precursor protein was synthesized from plasmid OTC with the TNT Quick Coupled Transcription/Translation kit (Promega #L2080). Upon import into the mitochondrial matrix ...
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bioRxiv - Systems Biology 2023Quote: The concentration of glutamine and glutamate in the media were measured using the luminescence-based approach Glutamine/Glutamate-Glo TM Assay kit (Promega). The method is based on two steps ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Microbiology 2024Quote: ... for intra-subcellular (i.e., vacuolar) conversion of glutamine (Gln) to glutamate (Glu) assay using Glutamine/Glutamate-Glo Assay kit (Promega, Madison, WI; Cat. # J8021) following kit’s protocol ...
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bioRxiv - Immunology 2021Quote: ... while glutamate levels were measured using Glutamine/Glutamate-Glo™ Assay kit (Promega). αKG was measured using a colorimetric assay kit (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lactate-GloTM and Glutamine/Glutamate-GloTM assay kits were purchased from Promega (Madison, WI). The perforin ELISA kit and Fixable Viability Dye eFluor 780 were purchased from eBioscience (St ...
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bioRxiv - Developmental Biology 2024Quote: ... and the Glutamine/Glutamate-Go assay (J8021) assay kit from Promega (cat. no. J8021), respectively ...
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bioRxiv - Microbiology 2024Quote: ... a gamma activated sequence firefly luciferase reporter plasmid (pGL4[luc2P/GAS RE Hygro] (Promega), and a constitutively expressing Renilla luciferase reporter plasmid pRL-TK (Promega).
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Berberine is a novel mitochondrial calcium uniporter (MCU) inhibitor that disrupts MCU-EMRE assemblybioRxiv - Cell Biology 2024Quote: ... biotin or biotin-labeled berberine (2 μg) was pre-incubated with either recombinant protein (2 μg) or in vitro transcribed/translated protein (Promega, Cat# L1170) for 8 hours at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Glutamate and glutamine concentration was determined using the Glutamine/Glutamate-Glo Assay (Promega). Metabolite concentration was normalized by the cell number at each time point.
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bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... Tris(2-carboxyethyl)phosphine (TCEP) and BCA protein assay kit were from Pierce and sequence-grade trypsin was from Promega. Rapigest and Sep-Pak C18 columns were from Waters and C18 Zip tips were from Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were digested with 2 μg of trypsin (Promega, WI) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were digested overnight at 37°C with 2 µg trypsin (Promega). Phase transfer surfactants (such as SLS ...
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bioRxiv - Neuroscience 2021Quote: The glutamine-glutamate-glo assay (Promega, # J8021) was performed according to manufacturer’s instructions to determine intracellular glutamine and glutamate concentrations ...
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bioRxiv - Neuroscience 2023Quote: A Glutamine/Glutamate-Glo™ Assay (Promega) was used to measure intracellular concentrations of glutamine and glutamate ...
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bioRxiv - Neuroscience 2021Quote: ... of miR-3594-5p was amplified from rat brain cDNA and cloned downstream of the Renilla luciferase reporter gene into the psiCHECK-2 vector (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... and protein-protein interactions were analyzed with NanoBRET™ Nano-Glo® Detection System kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Developmental Biology 2021Quote: ... media was collected at a single timepoint (12 hours) and the Promega Glutamate/Glutamine-Glo kit (Promega cat. no. J8021) was used ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Immunology 2020Quote: ... following the Glutamine/Glutamate Glo assay manufacturer’s protocol (Promega). Upfront sample processing such as dilution is required to fit in to the linear range of the glutamine or glutamate standard curve ...
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bioRxiv - Microbiology 2023Quote: ... Cell supernatants were assayed for glutamine consumption (Promega #J8021) and Lactate production (Promega #J5921 ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Immunology 2024Quote: ... Supernatants are then transferred to a fresh 96-well plate for storage and assayed for IL-1β (canine, monkey, porcine, rabbit or rat; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). Once supernatants are removed ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates from the single cells were digested with 2 ng of Trypsin (Promega, Cat. V5280), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...