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Citations for Promega :
201 - 250 of 8899 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 1 µl of duplex DNA was ligated into 25 ng of pBract214m-HvCas9-HSPT vector linearized with AarI using the ligation reagents and protocol described in pGEM®-T Easy Vector System kit (Promega). The ligation reaction was incubated overnight at 4°C then transformed into competent E ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml basic fibroblast growth factor (catalog no. G507A, Promega). Human primary myoblasts were passaged the same way as C2C12 cells ...
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bioRxiv - Neuroscience 2023Quote: ... and w/wo 100 ng/mL Nerve Growth Factor 2.5S (Promega, #G5141).
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bioRxiv - Microbiology 2020Quote: Genomic DNA fragments generated by PCR were cloned into the pGEM-T Easy plasmid vector using the pGEM®-T Easy Vector System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: The prepared PCR fragments were inserted into the pGEM-T vector via T/A cloning following the manufacturer’s recommendations (Promega, Fitchburg, USA). Competent Escherichia coli were transfected with the ligation mix and plated on agar plates as described previously [13] ...
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bioRxiv - Biochemistry 2020Quote: ... Two control reactions were performed: one containing 8 units of HeLa nuclear extract provided by Promega as a positive control and the other containing no nuclear protein as a negative control ...
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bioRxiv - Systems Biology 2022Quote: Cell Titer Glo Luminescent Cell Viability Assay Kit (Promega) was used and subsequently performed after the BRET measurement ...
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bioRxiv - Molecular Biology 2023Quote: ... ATRX 5’-TGAAACTTCATTTTCAACCAAATGCTC-3’ and 5’-ATCAAGGGGATGGCAGCAG-3’ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Equilibrated cells were transfected with 0.8 μg of 5-HT1eR or 5-HT1FR and 8 μg of GloSensor plasmid (Promega), after mixing with 17.6 μl of PEI in OptiMEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μL of CellTiter-Fluor Cell Viability Assay reagent (Promega) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... which is approximately a 5:1 ratio of effector cells (Promega) to target cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% CO2 and cell viability was measured using CellTiter-Glo (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with 5 μM Z-VAD-FMK (Promega G7231) or DMSO vehicle control ...
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bioRxiv - Cell Biology 2023Quote: ... Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Genomics 2019Quote: ... The A-tailed PCR products were then ligated to pGEM®-T Easy Vector using pGEM®-T Easy Vector Systems (Cat. A1360, Promega). Positive colonies were selected for Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... or the region between abaR and the abaI (for the abaI fusion) were amplified by PCR and ligated in pGEM®-T Easy using the pGEM®-T Easy Vector System (Promega). The resulting plasmids and the promoterless luxCDABE operon were digested with KpnI and BamHI and ligated in order to introduce the lux operon downstream of the predicted promoter of abaM or abaI ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed with TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse IgG (diluted 1:1,000 in TBS-T) (Promega, Madison, WI, USA) for 1 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and then cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
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bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... using the pGEM-T Easy Vector Systems protocol (Promega) and an LB-ampicillin selective medium ...
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bioRxiv - Molecular Biology 2019Quote: ... Products were cloned into pGEM-T Easy vector (Promega). Bacterial colonies obtained after transformation were screened by colony PCR for the presence of inserts of appropriate size ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy vector (Promega, Madison, WI, USA) were amplified by PrimeSTAR® MAX DNA Polymerase (TaKaRa Bio ...
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bioRxiv - Microbiology 2019Quote: ... which was then ligated into pGEM-T vector (Promega), generating pGEM-T-Δslo-2kb ...
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bioRxiv - Genetics 2019Quote: ... purified and cloned into pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Microbiology 2019Quote: ... These amplicons were cloned into pGEM-T Easy (Promega), and transformed into E ...
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bioRxiv - Genetics 2020Quote: ... A DNA fragment containing 200 bp of upstream HO flanking region and 200 bp of the HO gene was synthesized by Life Technologies and cloned into pGEM®-T Easy (Promega Corp.) as a NotI fragment (pCB4386) ...
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bioRxiv - Physiology 2019Quote: ... cloned into pGEM-T vector (Promega, Madison, WI, USA) and nucleotide sequence was confirmed by Sanger sequencing (Center for Applied Genomics ...
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bioRxiv - Genetics 2021Quote: ... and ligated into pGEM®-T Easy vector (Promega) using 1:3 molar vector:insert ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Amplicons were cloned into pGEM-T Easy vector (Promega) and sequenced.
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the pGEM-T Easy Vector (Promega) using DNA Ligation Kit
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bioRxiv - Microbiology 2020Quote: ... using the pGEM®-T easy Vector System (Promega). Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... sub-cloned into the pGEM T-Easy Vector (Promega), amplified with primers attB1 5’-TTACTCCATGTGTCAATACCAAAA-3’ and attB2 5’-GTCCATTTTAGTTCTCGAGTCGG-3 and introduced into the pDONR207 Gateway donor vector (Invitrogen) ...
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bioRxiv - Pathology 2022Quote: ... and cloned into a pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Genomics 2022Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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Cloning and In silico characterization of Heat shock factor (Hsf2) from Wheat (Triticum aestivum L.)bioRxiv - Molecular Biology 2020Quote: ... and cloned into pGEM-T simple vector (Promega, USA) in Escherichia coli strain M13 for sequencing.
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bioRxiv - Microbiology 2020Quote: ... cloned into pGEM-T-Easy (Promega - Madison, Wisconsin, USA) according to the manufacturer’s protocol and transformed into E ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Developmental Biology 2019Quote: Probe inserts were ligated into pGEM T-easy (Promega) according to kit instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Amplicons were ligated into pGEM T-easy vector (Promega) and Sanger sequenced.
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bioRxiv - Neuroscience 2021Quote: ... and cloned into the pGEM-T Easy vector (Promega) following manufacturer instructions and verified by sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... subcloned into the pGEM-T Easy Vector System (Promega) and sequenced on an ABI 3730 sequencer.
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bioRxiv - Plant Biology 2021Quote: ... pHSVd-monomer was based on pGEM-T vector (Promega) via insertion of HSVd cDNA cloned from HSVd-RZ plasmid via HSVd-f and HSVd-r primers (Supplemental Table 3) ...
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bioRxiv - Developmental Biology 2021Quote: ... then cloned into the pGEM-T easy vector (Promega). bmp2b and bmp4 were kindly provided by Matthias Hammerschmidt (Hild et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and cloned into pGEM®-T Easy Vector (Promega) using standard cloning procedures.
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bioRxiv - Genomics 2021Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... and cloned into the pGEM-T Easy vector (Promega). After sequence verification ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned into vector pGEM-T (Promega). CRISPR A and B start naturally with a 6 nt cassette consisting of adenines and guanines that we used for synchronisation of transcription (see below) ...