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Citations for Promega :
1 - 50 of 8899 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Ca2+-dependent nuclear factor of activated T-cells (NFAT) (pGL4.30; Promega), or the MAPK signaling pathway (pGL4.33 ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Genomics 2023Quote: ... The total cellular DNAs of the activated CD4+ T cells were isolated using a DNA extraction kit (Promega Wizard) for analysis.
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bioRxiv - Cell Biology 2019Quote: ... Cells were either treated with 50ng/ml rat Nerve Growth Factor (NG; Promega, Madison, WI, USA) at 0 ...
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
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bioRxiv - Immunology 2021Quote: The T Cell Activation Bioassay kit was purchased from Promega. The Jurkat-NFAT cells were thawed and cultured in RPMI (supplemented with 10% FBS and 5% pen/strep ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Cancer Biology 2021Quote: T Cell Activation Bioassay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Physiology 2023Quote: ... UMR106 cells were plated at 4 × 105 per well in a 6-well plate and transfected with 2 µg of NFAT5 gRNAs per well using the FuGENE® HD Transfection Reagent (Promega, USA). 24 h post-transfection ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or a pGEM®-T Easy Vector (pGEM®-T Easy Vector Systems Kit, Promega, USA) depending on the Taq-polymerase used for PCR (blunt vs ...
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bioRxiv - Biochemistry 2020Quote: ... The pGEM-T Vector kit was purchased from Promega. Acrylamide/bisacrylamide (29:1 ...
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the pGEM T-Easy vector kit (Promega). Clones were fully sequenced and confirmed as the targeted sequence ...
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bioRxiv - Microbiology 2021Quote: ... and pGEM®-T vector kit (Promega, Madison, Wisconsin, USA), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... a ~600 bp mouse ATF6β cDNA fragment was PCR-amplified using 5’-AACAGGAAGGTTGTCTGCATCAT-3’ and 5’-GTATCCTCCCTCCGGTCAAT-3’ primers and inserted into the pGEM-T vector (Promega). The plasmid was linearized using EcoRV and ApaI to synthesize the antisense and sense probe ...
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bioRxiv - Genetics 2021Quote: ... A ~1 kb fragment of the blm cDNA was cloned using primers blm-F – 5’-GGAGTCGAAACACCTGGTGGTA-3’ and blm-R – 5’-CTCATCAATGACCAAGCGAGCC-3’ into pGEM-T vector (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Biophysics 2021Quote: ... was amplified from cDNA samples using primers SC2-protN28182-F (5’-AGTCTTGTAGTGCGTTGTTCG-3’) and SC2-protN29566-R (5’-ATAGCCCATCTGCCTTGTGT-3’) and cloned into pGEM-T Easy (PROMEGA - USA), generating plasmid pGEM-SC2-N ...
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bioRxiv - Developmental Biology 2022Quote: ... CNS1 was amplified by PCR from Xenopus laevis genomic DNA using primers 5’-CCGCTCGAGCAGAGCAGACAGGGTCTGTA −3’ and 5’-CCCAAGCTTTGACCGTCAGTTTCATGACT-3’ and inserted into pGEM®-T Easy vectors (PROMEGA). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... or pGEM vector using pGEM-T easy vector system kit (Promega). Inserted sequences were excised by digestion with ClaI and inserted into the ClaI site of the replication-competent retroviral vector RCASBP(A) ...
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bioRxiv - Molecular Biology 2022Quote: ... Bands <2kb were cloned using the pGEM-T Easy kit (Promega) and bands >2kb were cloned using the Topo-XL2 PCR kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... We used the pGEM® T-Easy Vector System kit (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Microbiology 2020Quote: ... and cloned into pGEM-T in JM109 competent cells (Promega). C ...
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bioRxiv - Neuroscience 2023Quote: ... A fragment of the rat Piezo 1 cDNA was amplified with Taq Polymerase and the oligonucleotide primers 5’-GAGGAAGAGGACTACCTT and 5’-TTTACTTAGAAAACCCTACAG from bladder total RNA and cloned into the pGEM-T Easy vector (Promega, Madison, WI). Sequence was confirmed by Sanger capillary sequencing and sense and antisense RNA probes were synthesized with T7 and SP6 RNA polymerases (Roche-Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: The ability of T cells to lyse organoids was also measured using LDH-Glo™ Cytotoxicity Assay Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Cells were seeded on 24-well cell culture plates and transfected in triplicates with 20 ng of firefly luciferase reporter pGL3 β-catenin-activated reporter (pBAR) (19099249) and 5 ng of renilla luciferase reporter pGL4.73 hRLuc/SV40 (RLuc; Promega, WI, USA) and a membrane GFP (75 ng as control for transfection ...
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bioRxiv - Genetics 2019Quote: ... The 5 kb CcMoY fragment was then cloned in the pGEM®-T Easy vector (Promega) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the PkHSP70 5’UTR sequence was amplified and inserted into a pGem-T Easy vector (Promega) followed by the Cre60 sequence via restriction cloning between XhoI/KpnI sites ...
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bioRxiv - Immunology 2021Quote: CD8+ T cell cytotoxicity was assessed using a Cyto Tox 96® Non-radioactive Cytotoxicity Assay kit (Promega, Cat no. G1780) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... T cell cytotoxicity was assessed by quantification of LDH levels in the supernatants using the LDH-Glo cytotoxicity kit (Promega, J2380) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Microbiology 2023Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Immunology 2021Quote: ... luciferase was activated with the Luciferase Assay System (Promega), and relative light units (RLU ...
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bioRxiv - Microbiology 2022Quote: ... luciferase was activated with the Luciferase Assay System (Promega), and RLUs were measured at 570 nm on a Spectramax L luminometer (Molecular Devices) ...
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bioRxiv - Molecular Biology 2022Quote: ... 7 and 10 were either cloned with the pGEM®-T kit (Promega) to assay individual sequences and/or sequenced by Illumina (Centre d’expertises et de services Genome Québec) ...
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bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or into the pGEM-T or pGEM-T Easy vector (Promega) and subsequently sequenced (Macrogen).
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Systems Biology 2021Quote: β-catenin activated Luciferase reporter assays in RKO-pBAR cells were performed using a Dual-Luciferase Reporter Assay System (Promega,) and following the protocol provided by the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... The amplicons were subcloned using pGEM-T Easy Vector Systems Kit (Promega, Tokyo, Japan) or Zero Blunt PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Each product was cloned using the pGEM®-T Easy vector kit (Promega Corp.) to derive 12 clones using the GeneJet plasmid purification kit (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested day 1 and day 5 post-infection using a CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were incubated for 72 hours prior to flow cytometry analysis or Cell Titer Glo (Promega) analysis.
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bioRxiv - Microbiology 2020Quote: ... and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega, WI, USA). Purified PCR fragments and pGEM cloning 5’-terminal sequence of BToV were sequenced using primers designed for every ∼300 bp of the BToV genome ...
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...