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Citations for Promega :
651 - 700 of 1320 citations for Rat NDUFS4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 minutes before adding the transfection mix to the cells ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... together with pOG44 (Flp recombinase) plasmid using Fugene HD as per Promega protocol (Promega, Alexandria ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA was isolated using a Wizard Plus SV minipreps DNA purification kit (Promega) as per the manufacturers protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid constructs (pCAG nHA GPC4, see Table 1) were transfected with Fugene (Promega E2691)/ opti-MEM mixture according to manufacturer protocols ...
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bioRxiv - Genomics 2020Quote: ... pCGL-1-SNORD96A or empty pCGL-1 plasmid using Fugene (Promega, Madison, WI, USA). Cells were harvested 24- and 48-hours post-transfection for RNA isolation (TRIzol ...
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bioRxiv - Molecular Biology 2021Quote: ... and plasmids were purified using a Miniprep DNA purification kit (Promega Madison, WI, USA), followed by digestion with NdeI and XhoI (for gyrA)/ HindIII (for gyrB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with a total of 1μg combined DNA plasmids using FuGENEHD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µg of plasmid was mixed with 30 µL of FugeneHD (Promega, Cat# PAE2311) in Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: Plasmid DNA was isolated using Qiagen Midi columns or Wizard mini prep columns (Promega). Specific DNA fragments were isolated using an agarose gel extraction kit (Roche Applied Science) ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg pΔ8.91 packaging and 16 µg lentiviral vector plasmid using Fugene (Promega, #E2312) according manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Plasmids encoding for NanoBiT system (SmBit and LgBit) were purchased from Promega (Madison, USA) and cloned upstream of the tau protein in pcDNA3.1 vectors by restriction enzymes ...
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bioRxiv - Genomics 2023Quote: ... These sequences were inserted into the hPGK promoter of the luciferase plasmid pGL4.53 (Promega) using Gibson assembly ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
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bioRxiv - Microbiology 2023Quote: ... and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Microbiology 2023Quote: The pOXA-48TET plasmid was extracted using the Wizard genomic DNA purification kit (Promega). The pUN4 and pBAD18 derivatives were isolated using the Plasmid Easypure isolation kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin free plasmid for transfection was purified using PureYield column kits (Mini/Maxi, Promega). Plasmid DNA for direct use in electroporation of iPSCs was further concentrated by precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, A1470) and validated by restriction digest and sequencing ...
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bioRxiv - Molecular Biology 2023Quote: HEK293-µOR cells were transfected with a plasmid encoding pGloSensor-20F cAMP reporter (Promega). Cells were harvested 24 h post transfection and resuspended at a 1.5x10^6 live cells/ml in assay media (DMEM without phenol red or FluoroBrite DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
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bioRxiv - Cell Biology 2023Quote: ... with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega; E2311) to produce lentivirus ...
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bioRxiv - Microbiology 2023Quote: ... and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The plasmid sequence was verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Genetics 2024Quote: ... transfected with 4 µg of plasmid DNA using the FuGENE® HD Transfection (Promega) for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: Transient plasmid WT-PolK GFP 54 transfections were performed using FuGene six reagent (Promega), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... we constructed reporter plasmids based on pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega). We used ClonExpress II One Step Cloning Kit (Vazyme ...
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bioRxiv - Genetics 2023Quote: ... A Thymidine Kinase (TK) promoter-driven Renilla luciferase plasmid pGL4.74 [hRluc/TK] (Promega, #E6921) was used as a transfection efficiency control.
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bioRxiv - Biochemistry 2024Quote: ... The NanoLuc coding sequence including linker was obtained from the pNLF1-C plasmid (Promega) and inserted at the C-terminus of the TDO2 gene sequence (Flash Therapeutics ...
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bioRxiv - Biochemistry 2024Quote: ... The expression plasmids were transiently transfected by using FuGENE HD (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... All plasmid cDNA transfections were performed using the FuGENE HD Transfection Reagent (E2311; Promega) in Opti-MEM (31985070 ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection of HeLa cells with VPS35 plasmids was achieved using FuGene HD (Promega, #E2311) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Twenty-five ng of a plasmid encoding Renilla luciferase under CMV promoter (pRL-CMV, Promega) was co-transfected for normalization purposes ...
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bioRxiv - Molecular Biology 2021Quote: HTR cells were transfected with the indicated plasmids using TransFast transfection reagent (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... upstream of a luciferase reporter molecule in the context of a pGL3 plasmid (Promega, E1751). Plasmids designed for whole zebrafish injection were generated by replacing the firefly luciferase reporter with an enhanced green fluorescent protein (eGFP ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmid transfections were carried out with Fugene® 6 Transfection Reagent (Promega, Madison, WI, USA) with the manufacturer’s protocol.
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Membrane integration and topology of RIFIN and STEVOR proteins of the Plasmodium falciparum parasitebioRxiv - Biochemistry 2019Quote: ... Plasmid pGEM1 and TNT SP6 Quick Coupled Transcription/Translation System were purchased from Promega (USA). All enzymes were from ThermoFisher Scientific (USA ...
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bioRxiv - Molecular Biology 2019Quote: ... The linearized plasmid was treated with 2 units of shrimp alkaline phosphatase (Promega, Madison, USA) at 37 °C for 30 min ...
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bioRxiv - Genetics 2019Quote: ... The resulting PCR products were inserted by TA cloning into pGEM-T Easy plasmid (Promega) possessing T7 and SP6 promoters ...
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bioRxiv - Biochemistry 2020Quote: FLAG-β2AR HEK 293 cells were transfected with 3 µg cAMP luciferase plasmid DNA (Promega) using Turbofect (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... pCAGGS-Shisa7-IRES-GFP or pCAGGS-Shisa7S405A-IRES-GFP plasmids using FuGENE® HD (Promega). All recordings were performed 24 h post-transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting pX330 plasmids were transfected in the appropriate mESC lines using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: The HSV-TK promoter was cloned upstream of luciferase in the pGL4.10[luc2] plasmid (Promega). A 122 bp region of the E2f7 promoter or a 2.1kb region of the E2f8 enhancer was cloned into the XhoI site upstream of the HSV-TK promoter ...
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bioRxiv - Cell Biology 2021Quote: Transfection of plasmids into SUM159 cells was performed with FuGENE HD transfection reagent (Promega Corporation) ∼24 h prior to imaging.
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bioRxiv - Systems Biology 2021Quote: ... Transformations with the correct sequence were maxiprepped with PureYield™ Plasmid Maxiprep System (Promega # A2392) and utilized for lentiviral production.
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bioRxiv - Genomics 2021Quote: ... which was subsequently cloned downstream of Luc+ Poly (A) in the pGL3 promoter plasmid (Promega) and named as Ens-E ...
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bioRxiv - Microbiology 2020Quote: ... These fusions were expressed either from a plasmid based on the pGEM-T backbone (Promega) named pOP plasmid (Daskalov et al ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfections required 3 μg/well and were carried out using 0.1% Fugene HD (Promega).
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bioRxiv - Cancer Biology 2021Quote: The coding sequence of the mature IL-18 was cloned into pET-20b plasmid (Promega) before being transduced into E ...
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bioRxiv - Cancer Biology 2020Quote: Luciferase activity was measured 24 hours after transfection of equimolar amounts of psiCHECK2 plasmids (Promega) containing either the TP53 3′UTR or dUTR sequence downstream of the Renilla luciferase translational stop codon ...