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Citations for Promega :
501 - 550 of 1320 citations for Rat NDUFS4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with plasmid DNA using Fugene 6 reagent (Promega) by following the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmid DNAs were transfected into HEK293 cells with ViaFect Transfection Reagent (Promega) according to manufacturer’s instruction.
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bioRxiv - Synthetic Biology 2020Quote: ... either murine Il1rn or firefly luciferase from the pGL3 basic plasmid (Promega), and an NF-κB-negative regulatory element (NRE-5’-AATTCCTCTGA-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... 250 ng each of the pHMRluc (1) and pGL3-control plasmids (Promega) were added in addition to the indicated amounts of SP expression constructs.
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was purified with a Wizard Plus SV Miniprep kit (Promega). Restriction enzyme digestions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific or NEB) ...
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bioRxiv - Immunology 2021Quote: ... 4D6 cells were co-transfected with a renilla control plasmid (pRL Promega), and a luciferase reporter plasmid (pGL4.10[Luc2] ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.2 μg of pMD2.G envelope plasmid using FuGENE 6 (Promega). Cells were incubated up to 48 hrs and then lentivirus-containing media was collected and concentrated with Lenti-X concentrator (Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... 293T/17 and HeLa cells were transfected with plasmids using FugeneHD (Promega) or Lipofectamine3000 (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2019Quote: ... Assays were normalised by co-tranfected with renilla luciferase plasmid pGL4.74 (Promega) or by assaying total protein in extract ...
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bioRxiv - Bioengineering 2021Quote: ... All plasmids used for transfection were amplified using a Maxiprep kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... The pEYKBA plasmids were transfected into 293T cells using FuGENE HD (Promega) as described in section 2.2.3 ...
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bioRxiv - Microbiology 2021Quote: ... All plasmids used for transfection were amplified using a Maxiprep kit (Promega), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Each transfection consisted of 75 ng of luciferase reporter plasmid psiCHECK2 (Promega) or luciferase-5boxB plasmid psiCHECK2 −5boxB ...
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bioRxiv - Immunology 2021Quote: ... and HIV Rev plasmid using Fugene 6 transfection reagent (Promega, Madison, WI). Virus-containing medium was titrated to ensure undersaturating conditions for infection of H1299 cells ...
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bioRxiv - Biophysics 2022Quote: ... and PiggyBac transposon plasmid containing puromycin selection using FuGENE (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... containing flanking sequences that matched the pNL3.2NF-κB-RE plasmid (N111A, Promega). The pNL3.2NF-κB-RE plasmid was PCR amplified with primers containing sequences flanking the 5’UTR ...
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bioRxiv - Molecular Biology 2023Quote: ... the psPax2 envelope and the vsv-G packaging plasmids using Fugene6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... HIV-LTR plasmid constructs were transfected into Jurkat cells using ViaFect (Promega), 24 h post transfection cells were treated with GSK2981278 (40 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the plasmid of interest using Fugene 6 transfection reagent (Promega). All shRNA plasmids used were purchased from Sigma ...
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bioRxiv - Microbiology 2023Quote: The TRPPCa reporter plasmid p9X-NL1.2 was based on pNL1.2[NlucP] (Promega) that encoded Nanoluciferase fused to a C-terminal PEST sequence from mouse ornithine decarboxylase ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transfected into HEK293T cells using FuGENE HD (Promega, E2312) and proteins were allowed to express for 20h ...
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bioRxiv - Microbiology 2023Quote: Nanoluciferase (Nluc) gene was obtained from the pNL1.1 plasmid (Promega Corporation, USA). The nanoluciferase encoding gene was fused to the C-terminus of either CFP-10 or Rv1636 following a short linker sequence (GGSGGGSSGG ...
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE® 6 transfection reagent (Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA was purified from minipreps with QIAprep spin Miniprep Kit (Promega), and constructs were verified by sequencing (Microsynth).
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bioRxiv - Biochemistry 2023Quote: ... they were transfected with indicated plasmids using FuGENE 6 transfection reagent (Promega). After another 24h ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated using a PureYield Plasmid Midi Preparation Kit (Promega, USA) and clones verified via sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... the Cre plasmid was transfected using Fugene HD transfection reagent (Promega, E2311), then sorted out GFP+tdTomato− live cells by FACS (SONYMA900) ...
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bioRxiv - Cancer Biology 2023Quote: pRL-TK-renilla and pGL3 plasmids were obtained from Promega (Charbonnieres, France). pEF-cmyc-RIP140 and pEGFP-RIP140 have been previously described (31) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Properly sized products were ligated to the pGEM T-Easy plasmid (Promega), transformed into chemically competent DH5-alpha E ...
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bioRxiv - Microbiology 2023Quote: ... or 5 µL qPCR plasmid pJEB029 (diluted serially in lambda DNA (Promega)) were added ...
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bioRxiv - Cell Biology 2023Quote: ... we mixed 2500 ng of plasmid DNA with FuGene transfection reagent (Promega) and transfected 1 million Sf9 cells seeded in a 6 well plate ...
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bioRxiv - Bioengineering 2023Quote: ... Wizard® SV 96 Plasmid DNA purification system (Promega, Madison, WI, USA) was used for plasmid isolation ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with the plasmid using FuGENE HD (Promega, E2311). The indicated experiments were carried out 12–18 h after transfection ...
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bioRxiv - Systems Biology 2023Quote: ... Plasmid were delivered to cells using Fugene HD Transfection Reagent (Promega #E5912) at a 3:1 Fugene volume:DNA mass ratio for transfection complex formation in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific #31985070) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 μg of pCMV-VSV-G plasmids using FugeneHD (Promega, E2311) in Opti-MEM (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Neuroscience 2021Quote: ... of miR-3594-5p was amplified from rat brain cDNA and cloned downstream of the Renilla luciferase reporter gene into the psiCHECK-2 vector (Promega).
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bioRxiv - Evolutionary Biology 2020Quote: ... and the orthologous 208 bp blind mole rat sequence (nanGal1 assembly; KL203377:255659-255866) into a luciferase pGL4.23[luc2/minP] vector (Promega, US). We cloned the insert in the same orientation with respect to the minP promoter and luciferase gene as the CNE and the Tdrd7 promoter are oriented in the genome ...
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bioRxiv - Biochemistry 2022Quote: ... The half site ARE reporter is derived from the minimal rat probasin sequence (Zhang et al., 2000) and was cloned into pGL3 via KpnI and NcoI restriction sites (Promega). pRL-TK (Promega ...
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bioRxiv - Neuroscience 2021Quote: The Cacnb2 3’UTR (Ensemble ID: ENSG00000165995) was amplified from a rat brain cDNA library and cloned into the pmirGLO dual-luciferase expression vector (Promega) using the PfuPlus! DNA Polymerase (Roboklon) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μg of plasmid was transfected using Fugene HD (Promega, Madison WI, #E2311). Plasmid DNA was mixed with 8 µL Fugene and 100 µL media and incubated at room temperature for 15 minutes before being added to cells ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and siRNA transfections were performed using FuGENE 6 Transfection Reagent (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with the appropriate plasmid using the FuGeneHD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid transfection of HEK293F and HeLa cells was performed with Fugene HD (Promega), whereas SKBr3 cells were transfected using Fugene 6 (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection was performed with Jet PEI (PolyPlus Transfection) or Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... internal control plasmid pRL-TK (0.02 μg/well; Promega Corp.; Fitchburg, WI, USA), and the indicated expression constructs ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with the appropriate plasmids using Fugene HD Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified twice with a miniprep kit (PureYield™ Plasmid Miniprep System, Promega). Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E ...
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bioRxiv - Evolutionary Biology 2019Quote: Plasmid DNA was isolated using the Wizard Plus Minipreps DNA purification systems (Promega). Sequencing of the SHV gene was performed using M13 primers (MCLab ...