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Citations for Promega :
151 - 200 of 6784 citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... In-vitro translations were done with rabbit reticulocyte lysate (Flexi Rabbit Reticulocyte Lysate System, Promega) and translated CFTR (domains ...
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bioRxiv - Plant Biology 2022Quote: ... the 40 µl in vitro translation reactions contained 20 µM 19 amino acid mix (Promega) and 0.45 µCi/µl of [35S]-methionine (Hartmann Analytics) ...
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bioRxiv - Microbiology 2022Quote: ... expressed using the TNT SP6 High Yield Wheat Germ in vitro transcription/translation system (Promega) in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Bioengineering 2021Quote: ... Cell lysates were pre-cleared of any non-specific streptavidin-binding proteins by incubating with 60 µL of MagneSphere paramagnetic particles (Promega, Madison, WI) for 30 min with rotation ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... RRL in vitro translation reactions were carried out according to the manufacturer’s guidelines (Promega, Cat.No. L4960) for 50 min.
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bioRxiv - Cell Biology 2022Quote: IVTT reactions were performed using the SP6 TnT® Quick Coupled Transcription/Translation System (Promega #L2080) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... we used a control plasmid provided in the TnT® Quick Coupled Transcription/Translation System (Promega). The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of translation reaction was added to 30 μL Bright-Glo Luciferase assay reagent (Promega) and the resulting luminescence was measured in the illuminometer over a spectral wavelength of 350–650 nm and an integration time of 10 s at room temperature ...
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bioRxiv - Physiology 2022Quote: Synthesis of S35-labled CaVβ chimeras was done by coupled in vitro transcription-translation (Promega (L1170)) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged SPUD in vitro expression was done with Transcend Non-Radioactive Translation Detection System (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Sly1-HA3 was synthesized with the TNT® SP6 Quick Coupled Transcription/Translation system (#L2080, Promega), according to the instructions of the manufacturer ...
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bioRxiv - Synthetic Biology 2023Quote: Translation yield from the PURExpress reactions was quantified using Nano-Glo HiBiT Extracellular Detection System (Promega). To make 1X NanoGlo solution ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
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bioRxiv - Plant Biology 2020Quote: ... 10 µl of the translation reaction product was mixed into 50 µl of Luciferase assay reagent (Promega) and measured immediately on a GloMaxTM20/20 Luminometer (Promega) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega) and the Fluorotect GreenLys tRNA (Promega ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Cell Biology 2022Quote: Constructs for in vitro translations in rabbit reticulocyte lysate were based on the pSP64 vector (Promega, USA). Constructs for in vitro translation in the E ...
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bioRxiv - Physiology 2023Quote: ... 25 ng/mL fibroblast growth factor (bFGF; Promega), 1% penicillin streptomycin ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and biotinylated in a single reaction with the TnT® SP6 Quick Coupled Transcription/Translation System (Promega, L2080) and Transcend™ tRNA (Promega L5016) ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation of vvl/dfr vDNA was carried out using TNT coupled reticulo lysate system (Promega) and T3 RNA polymerase (New England Biolabs Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... were transcribed and translated using the TNT T7 Quick for PCR DNA Coupled Transcription/Translation System (Promega, USA). The reactions contained 10 µL of TNT ...
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bioRxiv - Microbiology 2021Quote: In vitro transcription and translation reactions were conducted using the TNT coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2μl of in vitro translation product was then mixed with 25μl of nano luciferase assay reagents (Promega N205A). Light production was measured on a luminometer ...
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bioRxiv - Biochemistry 2024Quote: ... In vitro translations were performed with wheat germ extract as directed by the manufacturer (Promega or tRNA Probes) with 3H leucine or 35S methionine ...
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bioRxiv - Physiology 2023Quote: ... the oligo with NF-κB consensus binding element (Promega) was end-labeled by T4 polynucleotide kinase (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Microbiology 2021Quote: ... Translation of the DNA library was performed using T7 RiboMAX™ Express Large-Scale RNA Production System (Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were added alone or in combination at the indicated concentrations (0.25-4 pmol) to in vitro translation reactions (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified constructs were used as inputs for reactions (final reaction concentration of 6.96 ng/µL) using the TnT Quick Coupled Transcription/Translation System (Promega). Reactions were performed as described for the TnT system ...
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bioRxiv - Cell Biology 2024Quote: ... Stag2 and Pds5b variants were first expressed by ‘cold’ coupled in vitro transcription- translation (IVTT; TNT reticulocyte lysate, Promega). IVTT aliquots (3 µl ...
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bioRxiv - Biophysics 2022Quote: ... Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) was used to cleave MBP-Q44-HttEx1 at 22 °C (Boatz et al. ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... samples corresponding to 4.44×105 cell equivalents of the translation reaction were put on ice and mixed with 1x Renilla-Glo substrate (Promega) in Renilla-Glo (Promega ...
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bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
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bioRxiv - Neuroscience 2020Quote: In vitro translation rates represent the increase in luminescence as a function of time using a standard rabbit reticulocyte lysate system (Promega) with luciferase mRNA (Promega) ...
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bioRxiv - Microbiology 2019Quote: In vitro translation reactions were performed using coupled bacteriophage T7 RNA polymerase transcription-rabbit reticulocyte lysate translation reactions as per the manufacturer’s instructions (Promega TNT). Samples were radiolabelled with 35S-methionine (Perkin Elmer ...
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bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
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bioRxiv - Biochemistry 2020Quote: All basic transcription-translation reactions for PrP constructs were performed using a Rabbit Reticulocyte Lysate (RRL) derived SP6 coupled system (Promega) for 30 min at 30 °C in the presence of 2.5 μCi [35S]-methionine in a total volume of 10 μl ...
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bioRxiv - Cell Biology 2021Quote: ... and Flag-tagged HGF WT and HGF 4Cys-4Ala were in-vitro translated (TNT quick coupled Transcription/Translation system, Promega) and were incubated with the bead bound c-MET for 4h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual-Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control WT replicon ...
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bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL in vitro translation reactions were performed in the linear range using 2.8 nM mRNA in the Flexi RRL System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Developmental Biology 2020Quote: ... SrpB (Waltzer et al 2002) and Med1A (Immarigeon et al 2019) by in vitro transcription/translation coupled reactions using rabbit reticulocyte extracts (TnT-Promega) isoforms labeled ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins used for EMSA experiments were synthesized in-vitro from pTNT plasmids using TNT T7 Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol ...