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Citations for Promega :
51 - 100 of 6784 citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Coupled in vitro transcription and translation assays were performed using the TNT Quick Coupled Transcription/Translation system (Promega) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: In vitro translation (IVT) of each eIF4E was performed using the TNT Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol and based on a protocol developed to study zebrafish eIF4E (111) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were either treated with 50ng/ml rat Nerve Growth Factor (NG; Promega, Madison, WI, USA) at 0 ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Cancer Biology 2021Quote: ... T7 expression vectors were used in an in vitro translation reaction using TNT Quick Coupled Transcription/Translation System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The binding proteins were eluted by trypsin (Promega Corporation, Madison, WI, USA) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pGEM-1 and the TNT SP6 Transcription/Translation System were from Promega. [35S]Met was from PerkinElmer.
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
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bioRxiv - Plant Biology 2019Quote: ... HA-tagged RPT2 N proteins were prepared with in vitro transcription and translation of pGADT7-RPT2 N using the TNT Quick Coupled Transcription/Translation Systems (Promega).
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bioRxiv - Immunology 2020Quote: Tle3 containing a flag tag in the C-terminus was cloned into the pTNT plasmid and transcription and translation was subsequently performed using the TNT Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... from designated translation reporter constructs were used for a 25 µL translation reaction volume using wheat germ extracts (WGE; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription and translation reactions using rabbit reticulocyte lysates as previously described (29) were performed by TNT Quick Coupled Transcription/Translation System (Promega) in the presence of [35S] Met (Perkin-Elmer) ...
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bioRxiv - Molecular Biology 2019Quote: ... using a coupled transcription-translation system containing wheat germ extract (TNT kit; Promega, Cat no. L5030), according to the manufacturer protocol.
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bioRxiv - Molecular Biology 2023Quote: The TnT quick coupled transcription/translation system (Promega) was used to generate 35S-labeled eIF4B ...
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bioRxiv - Cell Biology 2023Quote: In-vitro transcription and translation (IVTT) reactions were performed using the SP6 TnT® Quick Coupled Transcription/Translation System (Promega #L2080) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Molecular Biology 2021Quote: ... PGC-1α full-length and protein domain were translated in vitro in the T7 transcription and translation coupled reticulocyte system (Promega) supplemented with 35S-Methionine ...
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bioRxiv - Plant Biology 2020Quote: ... ZCCHC8B and RBM7 proteins were obtained by in vitro transcription and translation using the TNT T7 coupled wheat germ extract system (Promega) following the protocol supplied by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: Myc- and FLAG-tagged versions of the different ALOG proteins were produced using the Quick Coupled Transcription/Translation System (TnT; Promega). 25 µL of TnT reactions producing indicated proteins were mixed with Buffer 1 to reach 150 µL and rotated at 4 °C during 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced using 5 µg of plasmids in 25 µL in vitro translation reaction of TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega). For the OSH15-RI complex ...
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bioRxiv - Biochemistry 2020Quote: ... In vitro translation assays were run on the prepared mRNA templates using Rabbit Reticulocyte Lysate kits (Promega), with translation mixes prepared in triplicate as per manufacturer′s protocols (for S2D ...
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bioRxiv - Molecular Biology 2021Quote: ... we used TNT Quick coupled transcription/Translation System (Promega) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Nuclease-treated rabbit reticulocyte lysate translation reactions (Promega L4960) were set up in a 384-well plate (Thermo Scientific 164610 ...
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bioRxiv - Biochemistry 2021Quote: ... the TnT Quick Coupled SP6 Transcription/Translation System (Promega) was used by following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: In vitro translation reactions were performed in WGE (Promega) as described [93] ...
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bioRxiv - Cell Biology 2020Quote: ... the FluoroTect− GreenLys in vitro Translation Labeling System (Promega) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... After the translation reaction was quenched by adding 30 μl of 1× Passive Lysis Buffer (Promega), 10 μl of the mixture was placed on a 96-well white assay plate (Coster) ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Biochemistry 2021Quote: ... import assays of the comparatively higher molecular weight ACE2 and S proteins (50 μL reactions) were both performed using the TNT® Coupled Transcription/ Translation system (Promega) for 90 min at 30°C as described by the manufacturer (∼50 ng/μL cDNA,1 μM Ipom-F or an equivalent volume of DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Myc tagged full-length and truncated ARL13B fragments were generated by in vitro translation using TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Microbiology 2020Quote: ... or the TnT T7 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the T7 promoter ...
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bioRxiv - Developmental Biology 2019Quote: ... FluoroTect™ Green Lys in vitro Translation Labelling System (Promega) was used for protein labelling in place of 35 S-methionine in the original protocol ...
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bioRxiv - Cell Biology 2021Quote: A TNT T7 Quick Coupled Transcription/Translation System (Promega, L1170) with Transcend tRNA (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... the translation mixture was supplemented with FluoroTect GreenLys reagent (Promega). Purified samples were washed with TBS (50mM Tris and 150mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation was performed in rabbit reticulocyte lysate (Promega) supplemented with 1 mCi/ml [35S]Met/Cys ...
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bioRxiv - Cell Biology 2019Quote: ... in vitro translation was performed in nuclease-treated RRL (Promega) using 150 ng of luciferase mRNA in the presence of 15 μCi of [35S]-Met for 60 min at 30°C ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation assays with wheat germ extract (Promega®) were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Translation was performed in Flexi® Rabbit Reticulocyte Lysate (Promega) supplemented with amino acid mixture minus methionine and leucine ...
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bioRxiv - Bioengineering 2021Quote: ... a volume of binding buffer from PCR clean-up kit (Promega, A9282) was added followed by 2 µL (300 ng ...
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bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Cell Biology 2020Quote: ... GST fusion proteins were isolated by binding to glutathione magnetic beads (MagneGST™ Glutathione Particles, Promega) for 30 min at room temperature on a rotating wheel ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.