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Citations for Promega :
351 - 400 of 1243 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Coelenterazine-h substrate (2.5 µM) (#S2011, Promega, Madison, WI, USA) was added to the cells ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was obtained using MMLV H Minus Reverse Transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Microbiology 2019Quote: ... and 72 h using nonradioactive cell proliferation assay (Promega, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... cDNA was obtained using M-MLV H- reverse-transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Cancer Biology 2019Quote: ... treated RNA using MMLV RNase H minus reverse transcriptase (Promega) and random hexamer primers (Promega).
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... followed by 3 h at 37°C using trypsin (Promega). All proteolytic digests were acidified to pH 2 by addition of 10% formic acid and directly analyzed by LC-ESI-MS/MS ...
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bioRxiv - Microbiology 2019Quote: ... the pellet was washed once with 1.25X Buffer H (Promega) and resuspended in 500 µl of 1.25X Buffer H (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... and resuspended in 500 µl of 1.25X Buffer H (Promega). A total of 7.5 μl of SDS 20% was added and incubated for 40 min at 65°C and then for 20 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using M-MLV RNase H minus (Promega). cDNA products were purified on gel and amplified by PCR using the Phusion polymerase (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... 24 h later cells were transfected with Fugene HD (Promega) and constructs containing dCas9-VPR and gRNA vectors targeting the conserved DAR region (500ng plasmid DNA in molar ratios sgRNA:dCas9-VPR) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was obtained using M-MLV H-reverse-transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Molecular Biology 2023Quote: ... the strips were washed in TBS-T for 15 min and incubated with the secondary antibody anti-mouse IgG-HRP (Promega, Cat. no: W4028) at 1:10000 dilution in TBS-T 3 % BSA for 1h ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with anti-rabbit horseradish peroxidase (HRP) conjugate antibodies (1:20000 dilution) (Promega, Madison, WI, USA) for two hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and M-MLV Reverse Transcriptase (RNase H Minus, Point Mutant; Promega). Quantitative (q)RT-PCR reactions for target genes were prepared using specific primer (Oligomer ...
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bioRxiv - Immunology 2019Quote: ... and the luminescence after incubation with 5 µM coelenterazine H (Promega) were recorded using a Mithras LB940 reader (Berthold).
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bioRxiv - Microbiology 2021Quote: ... 48 h later cells were lysed with reporter lysis buffer (Promega) and assays were read on a FLUOstar Omega plate reader (BMF Labtech ...
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bioRxiv - Microbiology 2022Quote: ... 48 h later cells were lysed with reporter lysis buffer (Promega) and assays were read on a FLUOstar Omega plate reader (BMF Labtech ...
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bioRxiv - Developmental Biology 2020Quote: ... RNAs were reverse-transcribed using MMLV H(-) Point reverse transcriptase (Promega). Quantitative PCR was performed using the QuantiNova SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... template RNA was degraded by adding 2.5 U RNase H (Promega,) and 1µg RNase A (Pharmacia ...
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bioRxiv - Cancer Biology 2019Quote: ... Viability was assayed 72 h after plating using CellTiter-Glo (Promega).
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bioRxiv - Immunology 2020Quote: ... Infectivity was determined 72 h later by adding Bright-Glo (Promega) and measuring luciferase activity using a Synergy H1 plate reader (BioTek).
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bioRxiv - Molecular Biology 2023Quote: Luciferase activity was analyzed 48 h post-transfection using DualGlo (Promega), essentially as previously described (25) ...
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bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... 50 µ L of Kinase-Glo® reagent (Promega) was then added and incubated at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... digested using 20 ng/ l sequencing grade trypsin (Promega) for 1 hr at 47C ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
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bioRxiv - Genetics 2022Quote: ... membranes were washed and incubated with the appropriate HRP conjugated secondary antibody (1:3,000 anti-rabbit W401B antibody from Promega), and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
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bioRxiv - Plant Biology 2019Quote: ... during 4 h then with trypsin (Sequencing Grade Modified, Promega, Madison, WI) overnight ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 48 h later the cells were lysed in Reporter lysis buffer (Promega). Samples were then boiled in 5× sodium dodecyl sulphate (SDS ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assayed 120 h after induction using CellTiter-Glo (Promega). Luminescence signals of induced wells were normalized to uninduced well data.
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 μM coelenterazine h (Promega, Madison, WI, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL BSA (1 mg/mL) and 2 μL buffer H (Promega) in reaction volume of 20 μL ...
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bioRxiv - Genetics 2019Quote: ... RNA expression levels were quantified after reverse transcription with MMLV H-(Promega) using the TaqMan gene expression system (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... and 72 h later viable cells were detected using CellTiter Glo (Promega). Compounds were tested in a 16-point dose response (10 µM highest final concentration and 2-fold dilutions) ...
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bioRxiv - Microbiology 2021Quote: ... After 48 h 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior to reading on a BioTek plate reader ...
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bioRxiv - Microbiology 2022Quote: ... 48-55 h after infection Bright-Glo Luciferase Assay System (Promega, E2610) was used to measure luciferase activity ...
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bioRxiv - Microbiology 2022Quote: ... 24 h post transfection the Dual-Luciferase® Reporter Assay System (Promega) was used according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2023Quote: ... Cellular NAD(H) levels were measured using NAD/NADH-Glo assay (Promega). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... After 16 h cells were lysed in 1x passive lysis buffer (Promega) and firefly luciferase activity was measured after addition of firefly substrate-containing buffer (20 mM Tricine ...
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bioRxiv - Microbiology 2022Quote: ... As secondary antibodies α-mouse IgG HRP conjugate (Promega W4021) or α-rabbit IgG HRP conjugate (Cell Signaling #7074 ...
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bioRxiv - Biochemistry 2020Quote: hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Immunology 2023Quote: ... 50 ☐l of Bright-Glo Luciferase reagent (Promega E2620) was added to each well ...
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bioRxiv - Microbiology 2023Quote: ... Rabbit Reticulocyte Lysate (Promega), ATP regeneration system (0.5 mM ATP [Wako]) ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before re-suspending in 50 μl flow buffer containing anti-Nluc polyclonal primary antibody raised in rabbit (Kindly gifted by Promega) at 1:100 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... using one of the following antibodies against (all dilutions in PBS): HaloTag® polyclonal anti-rabbit (G928A, Promega, 1:100); ACTN2 monoclonal anti-mouse (EA-53 Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit polyclonal antibody was used followed by detection with a goat anti-rabbit secondary antibody conjugated to horseradish peroxidase and developed using a chemiluminescent substrate (Promega). Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day membranes were washed 3 times n TBTS for 5 minutes and incubated in secondary antibody (1:2500 anti-Rabbit HRP conjugated, Promega) diluted in blocking buffer for 1 hour at room temperature and washed 3 times with TBTS for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... IΒΑ1 (1:250, rabbit, Wako, 01919741) and p75NTR (1:1000, rabbit, G3231, Promega). Sections were washed 3×20 min in PBS prior to incubation with secondary antibodies (Alexa Fluor ...