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Citations for Promega :
651 - 700 of 1165 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The qRT-PCR was performed with GoTac® qPCR (Promega) with 10 ng cDNA template on a Biorad CFX384 Real Time Cycler ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quantitative real-time PCR was performed using GoTaq qPCR (Promega) BRYT Green Dye-based detection and CFAV NS3-specific primers ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR products were cloned into pGEM®-T plasmid (Promega) and transformed into TOP10 chemically competent E ...
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bioRxiv - Microbiology 2023Quote: ... and PCR amplified using the GoTaq qPCR system (Promega A6002) on a LightCycler 480 (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Master Mix (Promega, Madison, WI, USA), 3.94 μl of ddH2O ...
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bioRxiv - Microbiology 2019Quote: ... and analysed by PCR with GoTaq Master Mix (Promega) using the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... 5 µL of 5X GoTaq Green Master Mix (Promega), 0.125 µL of 5u/µL GoTaq G2 polymerase (Promega) ...
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bioRxiv - Genetics 2020Quote: Promega G2 green Taq 2x master mix (Promega, USA) = 5 µL for each reaction ...
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bioRxiv - Neuroscience 2020Quote: ... using GoTaq G2 Hot Start Green Master Mix (Promega), and 0.2 µM of common forward primer ...
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bioRxiv - Microbiology 2020Quote: ... a PCR reaction using the PCR Master Mix (Promega) and the primers rplU_Fwd and rplU_Rev (Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs were mixed with the GoTaq master mix (Promega), and specific primer pairs for relevant genes (Supplementary Table 1 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Following the GoTaq Green Master Mix (Promega, WI, USA) protocol ...
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bioRxiv - Immunology 2020Quote: ... SYBR green master mix (Cat#A6002, Promega, Madison USA), and nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... 1X GoTaq Green Master Mix solution (Promega, Wisconsin, USA) and nuclease-free water (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... using GoTaq G2 Hot Start Green Master Mix (Promega), and 0.2μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq Green master mix (Promega), per the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed using GoTaq PCR Master Mix (Promega) with primers mo37 and ag489 ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were performed using Master Mix (2X) (Promega) and carried out at an initial denaturation step of 95 °C for 2 min ...
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bioRxiv - Biochemistry 2020Quote: PCR was performed using GoTaq Green Master Mix (Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... We used the GoTaq® Green Master Mix (Promega) for colony PCRs ...
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bioRxiv - Microbiology 2023Quote: ... 40 μl TnT® Quick Master Mix (L1170, Promega), 1 μl Methionine (1 mM) ...
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bioRxiv - Cell Biology 2023Quote: ... Genotyping was performed using GoTaq Green master mix (Promega).
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bioRxiv - Bioengineering 2023Quote: ... 12.5 μL of GoTaq Green Master Mix 2x (Promega), and 5.5 μL of Ultrapure DNase/RNASE-Free Distilled Water (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL 2X master mixture (Promega, Madison, WI, USA), 1 µL forward and reverse primers (for each ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of GoTaq G2 Green Master Mix (Promega), and 200 µM of primers ...
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bioRxiv - Genetics 2019Quote: ... and cDNA synthesis (M-MLV RT kit, Promega Corporation, cat. #M170A) were performed according to manufacturer’s specifications [21] ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription was performed with an RT cDNA Synthesis Kit (Promega). Real-time PCR was performed with SYBR green SuperMix using an iCycler real-time PCR detection system (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... and reverse transcribed (RT) using the Reverse Transcription System kit (Promega). The resulting cDNA samples were amplified by TaqMan quantitative PCR using commercially available primer/probe kits from Applied Biosystems for BDNF (Gene Expression Assay Mm00432069_m1 ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR to validate splicing changes was performed using GoTaq (Promega) and quantified with Bioanalyzer RNA 6000 Nano assay (Agilent).
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bioRxiv - Genetics 2020Quote: RNA was reverse-transcribed using the GoScript RT kit (Promega A5000) following the kit protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RT-PCR analyses were performed using GoTaq DNA polymerase (#M7841, Promega) in a reaction volume of 25 μl comprising 1 μl of cDNA and primer pairs specific for the 5’-end (exon 1-3 ...
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bioRxiv - Genetics 2019Quote: ... Reverse-transcription (RT) was performed using the MMLV retro-transcriptase (Promega) from 500 ng of total RNA in a final volume of 60 µl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μl reverse transcription enzyme M-MLV-RT (Promega, USA). PCR was performed using each specific primer set in a total volume of 20 μl containing 10 pmol of each primer ...
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bioRxiv - Microbiology 2022Quote: ... treatment and cDNA was synthesized using M-MLV RT (Promega, USA). Gene specific primers used (Table 4) ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription (RT) was performed with M-MLV enzyme kit (Promega) and qPCR was conducted with the Syber GreenER qPCR Super Mix Universal (Invitrogen) ...
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bioRxiv - Physiology 2023Quote: ... cDNA was synthesized using Oligo-dTs and M-MLV RT (Promega). qRT-PCR was performed with the StepOnePlus System (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... digested overnight at RT with 500 ng of modified trypsin (Promega) in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2024Quote: ... The reaction consisted of 0.5 µL Luna RT (Promega Cat#E3005S), 2.5 µL of 2X reaction mix ...
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bioRxiv - Microbiology 2024Quote: ... Turbo-treated RNA was then reverse transcribed with AMV RT (Promega) primed with random 9-mer (IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... as well as Per2AS qPCR product cloned into pGEM-T (Promega). After combining 5’RACE and qPCR products in pBlueScript using Hind III and Xho I sites ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-96 real-time PCR System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed using the GoTaq qPCR SYBR mastermix (Promega) on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... qRT-PCRs were performed using GoTaq qPCR SYBR Green chemistry (Promega) and a Bio-Rad CFX 96 Touch System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... or directly used with the ONE STEP TAQ QPCR kit (Promega). For RT-qPCR assays ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was executed with the GoTaq qPCR Mastermix (Promega) employing the CFX-96 real-time PCR System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: Amplification was set up with the qPCR kit (Promega, Madison, WI) and carried out on an ABI7500 with an initial melting temperature of 95°C for 2 mins and 40 cycles of 95°C for 15 sec and 60°C for 1 min ...
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bioRxiv - Cell Biology 2019Quote: ... using SYBR Green GoTaq master mix from Promega (Cat.No. A6001). Calculation of relative gene expression was done after normalization to control rp49 using the ΔΔCt analysis method ...