Labshake search
Citations for Promega :
601 - 650 of 904 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... followed by a PBS wash and lysis buffer to measure the luciferase activity in cell lysates using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Immunology 2019Quote: 8 nM cap0 or 20 ng/μL VPg-linked RNA was translated using the Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence or absence of 1.5 μM Ifit proteins ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Microbiology 2019Quote: ... and both firefly and Renilla luciferase activities in the lysate were determined using the Bright-Glo and Renilla-Glo Luciferase Assay System (Promega), respectively ...
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bioRxiv - Microbiology 2020Quote: ... The former cell lysates were subjected to a luciferase assay to normalize the transfection efficiency using a firefly Luciferase Assay System (Promega) with a Centro LB960 luminometer (Berthold) ...
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bioRxiv - Immunology 2020Quote: ... The activity of Renilla luciferase in total cell lysates was measured with the use of a Dual-Luciferase Reporter Assay System (Promega) and was normalized by that of firefly luciferase derived from 80 ng of a control plasmid.
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bioRxiv - Immunology 2020Quote: ... the cells were lysed and luciferase activity was measured in the lysates using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLU ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blots to assess the abundance of the proteins were performed on the same volumes of lysates obtained from the luciferase measurements using the passive lysis buffer supplied with the Dual-Luciferase Reporter Assay System (Promega). Antibodies used for western blots were previously described (Grozdanov et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Firefly (Fluc)- and Renilla (Rluc)-luciferase activity in each cell lysate was determined using the Dual Luciferase Assay Kit (Promega) and a Berthold tube luminometer ...
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bioRxiv - Biochemistry 2020Quote: ... the culture medium was aspirated and 20 μL of cell lysate was added from the Luciferase Assay System (Promega, E1500) to each well ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... lysed in 100µl of passive lysis buffer and standard dual luciferase assays were performed on the cell lysates by using Dual-Luciferase Reporter Assay reagents (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared to consecutively measure the firefly and Renilla luciferase activities using Dual-luciferase reporter assay system (Promega) on INFINITE 200 Pro multimode reader (TECAN) ...
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bioRxiv - Cell Biology 2022Quote: ... Tg-NLuc abundance in the lysate and media were measured using the nano-glo luciferase assay system according to the manufactures protocol (Promega). Four controls were included for the experiments including a non-targeting siRNA control ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were analyzed 24 h after transfection to measure firefly and renilla luciferase activities using GloMax 96 microplate luminometer (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... FLuc and RLuc activities in lysates were sequentially measured by using Dual-Glo luciferase assay reagents and a GloMax 20/20 luminometer (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega), 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Cell Biology 2022Quote: ... in pcDNA3/V5-Dest40 vector were expressed in T7 rabbit reticulocyte lysates using TNT T7 Quick Coupled transcription/Transaltion System (Cat# TM045, Promega) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was determined 18-24 hr post-transfection from cell lysate using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturers protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Cell Biology 2023Quote: ATP was measured in MPs or cell lysates with the luciferase/luciferin method (Enliten rluciferase/luciferin cat. # FF2021, Promega, Italy) in a Firezyme luminometer (Biomedica Diagnostics Inc ...
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bioRxiv - Microbiology 2023Quote: ... and whole cell lysates prepared and assayed for luciferase activity using the Dual Luciferase Assay System as per manufacturer’s directions (Promega, USA). The luminescence signal was detected using the luminometer (Promega) ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were harvested 2 days hours following transfection and reporter assays were performed using the Dual-Luciferase Reporter Assay (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega).
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega). The ratio between Renilla and Firefly is calculated for each experimental condition.
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bioRxiv - Immunology 2023Quote: ... Protein expression in luciferase-based assays was verified by analyzing the corresponding lysates stored in 1x passive lysis buffer (Promega) by Tricine-SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were harvested and the activities of firefly and Renilla luciferase were measured in the same cell lysate using the Dual-Luciferase Reporter Assay System (Promega) and GloMax Luminometer (Promega) ...
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bioRxiv - Genetics 2023Quote: ... The HALO-NAC61 and GST-HALO (used as negative control) fusion proteins were translated in vitro using the TNTR SP6 coupled reticulocyte lysate system (Promega). Two replicates were used for the TF and input libraries ...
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bioRxiv - Developmental Biology 2023Quote: ... Oct4 was transcribed and translated in vitro in the presence of 35S-methionine (TNT Coupled Reticulocyte Lysate System, Promega, USA) according to the manufactureŕs protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and luciferase activities of cell lysate were recorded by using a dual-luciferase reporter assay system (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity in cell lysates was assayed 48 hours post-transfection using the Dual Luciferase Reporter Assay kit (Promega E1910), and the Fluoroskan Ascent FL microplate reader (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... LDH activity in the supernatants and the cell lysates was then assessed using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) by following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Cancer Biology 2023Quote: Caspase3/7 activity was quantified in cell lysates using the Kit Caspase-Glo® 3/7 Assay Systems (G8091, Promega).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wells containing single cell colonies were duplicated into 12-well plates and screened for HiBit expression by mixing crude lysates with purified LgBit protein (Promega) and measuring luminescence in the presence of 5 μM furimazine ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription and translation reactions using rabbit reticulocyte lysates as previously described (29) were performed by TNT Quick Coupled Transcription/Translation System (Promega) in the presence of [35S] Met (Perkin-Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo, Promega), and 10 μL of each lysate was added to 50 μL of 2 μg/mL Renilla luciferase substrate (NanoLight Technology) ...
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bioRxiv - Molecular Biology 2024Quote: ... at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega) and a luminometer ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the pSP64 in vitro translation vector and translated with a 35S radiolabelled methionine label using the TNT coupled reticulocyte lysate system (Promega) following manufacturers guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted with the ReliaPrep miRNA Cell and Tissue Miniprep System (Promega) and RNA quality was assessed with a Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted using the ReliaPrep™ RNA Tissue (Promega, Madison, WI, USA) following manufacturer’s instructions ...