Labshake search
Citations for Promega :
551 - 600 of 904 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... TUNEL staining on tissue sections was performed using the DeadEnd™ kit (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted following the Maxwell 16 LEV Tissue RNA Kit protocol (Promega). For tissue-specific assays ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA was robotically extracted using the Maxwell tissue RNA extraction kit (Promega) and quantified using the Nanodrop ND-1000 UV-Vis Spectrophotometer (Nanodrop technologies ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 10 mg leaf tissue was homogenised in 50 μL passive lysis buffer (Promega) containing protease inhibitor (P9599 ...
-
bioRxiv - Microbiology 2020Quote: ... Tissues were embedded in 3% low melting point agar (Promega, Madison, WI, USA). Formalin embedding ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA was extracted using the ReliaPrep miRNA Cell and Tissue Miniprep System (Promega). Knockdown of EWSR1-FLI1 was confirmed by qRT-PCR11 ...
-
bioRxiv - Microbiology 2019Quote: RNA was extracted using the Maxwell RSC simplyRNA tissue kit (Promega, Alexandria, NSW) on a Maxwell RSC system (Promega) ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA was isolated using the ReliaPrep RNA Tissue Miniprep System (Promega, Madison, WI). SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2022Quote: ... 50mg adipose tissue was homogenized in PBS containing protease inhibitor cocktail (Promega, G6521) in a Qiagen TissueLyser II (85300) ...
-
bioRxiv - Immunology 2023Quote: ... Total RNA was purified using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). The RNA was sent to Kazusa Genome Technologies to perform the library preparation and sequencing ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNAs were isolated using the ReliaPrep™ gDNA Tissue Miniprep System (Promega) following the Mouse tail protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Maxwell RSC simply RNA tissue extraction kit (Promega) with on-bead DNase I digestion ...
-
bioRxiv - Immunology 2023Quote: ... was isolated with Reliaprep RNA Tissue Miniprep System according to manufacturer instructions (Promega), followed by cDNA synthesis using GoScript Reverse transcription mix (Promega) ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated with the Maxwell 48 RSC simply RNA Tissue Kit (Promega) on a Maxwell RSC Instrument (Promega) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 20 mg of tissue was lysed with Passive Lysis 5X Buffer (Promega #E1941), and the lysate was incubated with H2O2 Substrate Solution at room temperature for 1 hour ...
-
bioRxiv - Cell Biology 2020Quote: ... 20 µL lysates were loaded into a 96 well plate and luminescence following administration of 50 µL Luciferase Assay Reagent II (Promega) and 50 µL Stop & Glo Reagent (Promega ...
-
bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... After 24 h luciferase activity was determined from at least four independent experiments with 20 μl of cleared lysate in an LB 9501 luminometer (Berthold) by using the luciferase assay system from Promega.
-
bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
-
bioRxiv - Molecular Biology 2021Quote: ... and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reporter gene expression was assessed in 20 µl of cell lysates using the Dual Luciferase Assay and a GloMax luminometer (Promega). To control for transfection efficiency ...
-
bioRxiv - Neuroscience 2020Quote: In vitro translation rates represent the increase in luminescence as a function of time using a standard rabbit reticulocyte lysate system (Promega) with luciferase mRNA (Promega) ...
-
bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by measruring ATP levels in cells lysates with a commercially available kit (CellTiter-Glo®, Promega).
-
bioRxiv - Microbiology 2020Quote: ... Transduction rates were assessed by measuring luciferase activity in cell lysates at 48 hours post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
-
bioRxiv - Microbiology 2020Quote: ... Infection rates were assessed by measuring luciferase activity in cell lysates at 16 h post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
-
bioRxiv - Genomics 2019Quote: ... Firefly luciferase and Renilla luciferase activities were measured in 10μl of HEK293T cell lysate using the Dual-Luciferase Reporter assay system (Promega, E1910) in a Veritas™ Microplate Luminometer ...
-
bioRxiv - Microbiology 2019Quote: In vitro translation reactions were performed using coupled bacteriophage T7 RNA polymerase transcription-rabbit reticulocyte lysate translation reactions as per the manufacturer’s instructions (Promega TNT). Samples were radiolabelled with 35S-methionine (Perkin Elmer ...
-
bioRxiv - Biochemistry 2020Quote: All basic transcription-translation reactions for PrP constructs were performed using a Rabbit Reticulocyte Lysate (RRL) derived SP6 coupled system (Promega) for 30 min at 30 °C in the presence of 2.5 μCi [35S]-methionine in a total volume of 10 μl ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 g of the muscle lysates were used for determining proteasome-associated chymotrypsin-like activity with the Proteasome-GloTM Assay Systems (#G8531, Promega). Luminescence signals were detected with the SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices ...
-
bioRxiv - Cancer Biology 2020Quote: ... 20 μL of cell lysate was transferred to 96-well plates and placed in a 96-well microplate luminometer (GloMax-Multi, Promega). 100 μL Luciferase Assay Reagent II and 100 μL Stop & Glo Reagent (both provided in the Dual-Luciferase Reporter kit ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cell lysates were transferred to a 96-well black plate and then an equal volume of Luciferase assay reagent (Promega) was added ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... the luciferase activities were measured in 20 μl of cell-lysate using the dual-luciferase assay system (Promega, Madison, WI) with a luminometer (LumiCount ...
-
bioRxiv - Synthetic Biology 2021Quote: All NanoLuc luciferase assays to quantify gene expression were performed with cell lysate according to the procedure described in the Nano-Glo Luciferase Assay System kit (Promega). For these experiments ...
-
bioRxiv - Systems Biology 2021Quote: ... Collisional energy was ramped stepwise as a function of ion mobility.24 Peptide loading was normalized to the total ion chromatograms from 200 ng injections of K562 whole cell lysate standard (Promega).
-
bioRxiv - Plant Biology 2021Quote: [35S]Met-labeled preproteins were in vitro-transcribed/translated using the TNT-coupled wheat germ or reticulocyte lysate system and SP6 or T7 RNA polymerase (Promega). Growth of Arabidopsis seedlings (for 14 days on MS agar media with 2% sucrose) ...
-
bioRxiv - Microbiology 2020Quote: ... the transduction efficiency was quantified by measuring the firefly luciferase activity in cell lysates using the firefly luciferase substrate of the dual-luciferase reporter assay system (Promega) and a Glowmax plate luminometer (Promega).
-
bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
-
bioRxiv - Microbiology 2020Quote: ... and luciferase activity was measured in 3 µl aliquots of the cell lysates using a Dual-Luciferase Reporter Assay System (Promega). Firefly luciferase activity was normalized with that of Renilla luciferase.
-
bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
-
bioRxiv - Cancer Biology 2021Quote: ... luciferase assay reagent was mixed in a 1:1 ratio with cell lysate (Luciferase Assay System kit, Promega, Madison, WI). Luciferase activity was measured with Synergy H4 Hybrid Reader (BioTek ...
-
bioRxiv - Cell Biology 2021Quote: ... 140 mM NaCl and 1% Triton X-100) and lysates used to assay luciferase activity in 96-well plates using the Dual-Luciferase Reporter Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... levels of Firefly and Renilla luminescence in cell lysates were measured by Synergy H1 microplate reader according to Promega’s Dual-Glo kit protocol (Promega #E2920). Fold changes of AP-1 activity were presented by the ratio of normalized Firefly/Renilla to control wells.
-
bioRxiv - Biochemistry 2022Quote: Radiolabeled proteins were synthesized in the presence of 35S-methionine and 35S-cysteine using either coupled reticulocyte lysate system (TNT, Promega) or uncoupled reticulocyte lysate system (Promega) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The lysate was washed in Nuclei wash/resuspension buffer (1xPBS completed with 1% BSA and 0.2U/ul RNAsin Plus (Promega, #N2611) and passed through a 40 μm cell strainer ...
-
bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
-
bioRxiv - Bioengineering 2020Quote: ... Cas13a mRNA was translated in vitro using the Rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, WI, USA). RNaseAlert®-1 Substrate was mixed with crRNA or NTCR (500 nM ...
-
bioRxiv - Microbiology 2022Quote: ... then cells were harvested and cell lysates were used to determine luciferase using a Dual-Luciferase Reporter Assay System (Promega). The firefly luciferase activities were normalized to Renilla luciferase activities.