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Citations for Promega :
301 - 350 of 1658 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus MMLV reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned into vector pGEM-T (Promega). CRISPR A and B start naturally with a 6 nt cassette consisting of adenines and guanines that we used for synchronisation of transcription (see below) ...
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bioRxiv - Microbiology 2023Quote: ... unlabeled PCR was cloned into pGEM-T Easy (Promega) and positive clones were checked by amplification with the vector-based primers M13f and M13R.
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bioRxiv - Physiology 2023Quote: ... The PCR products were subcloned to T-vector (Promega) and sequenced ...
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bioRxiv - Cell Biology 2022Quote: ... After PCR clean-up (Promega #A9281, Madison, WI, USA), A-tailing of the PCR products with Taq Polymerase was performed as ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq polymerase (Promega), 2.5 µl 10 µM forward and reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was performed using GoTaq Flexi DNA Polymerase (Promega), and PCR products were migrated on agarose gels ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The mutagenesis of the EPIYA sites were done one after the other using the kit Quick change II site-directed mutagenesis (Aligent #200523) ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The cagA fragment was digested with KpnI and EagI and subcloned into the lentivirus entry vector pEF-1α/pENTR (Addgene ...
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bioRxiv - Bioengineering 2023Quote: PCR was performed using GoTaq Flexi DNA polymerase (Promega) to amplify both junctions using primers listed in Table 1 and routinely 500 ng of genomic DNA from parental cells or each recombinant clone as template in 25 μl reactions ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... transferred to a PCR clean-up column (Promega, A9282) and centrifuged ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq Polymerase (Promega), 2.5 µl 10 uM forward and reverse primers ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2 ...
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCRs were conducted using GoTaq DNA Polymerase (Promega) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... and a Go-Taq real-time PCR system (Promega). The data were analyzed by the ΔΔCT method and normalized to ACT1 in each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 anterior and posterior scales from each fish were pooled for isolating the total RNA isolation in a single tube containing 0.25 mL of a tissue lysis buffer from Reliaprep RNA tissue miniprep system (Promega, #Z6111, USA) as well as one 1.4 mm ceramic bead to crush the scales ...
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bioRxiv - Microbiology 2021Quote: Swabs containing skin virome samples were saturated with 200µl of 0.02µm filtered sterile 1x PBS and were placed in a 2ml tube containing a CW Spin Basket (Promega, Madison, WI, USA). Swabs were centrifuged at 16000xg for 10 minutes to elute viral particles from the swab into the PBS solution ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant (700 μL) was transferred to a sterile Eppendorf tube and mixed with 50 μL protein precipitation solution (Promega, USA) by inverting it ten times before centrifugation (10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl of the RT product (diluted 1:5) was used for semi-quantitative PCR or qPCR reactions with Promega PCR Mix (Promega, Madison, Wisconsin, USA) and SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... amplicons from three PCR runs for each root sample were combined (Clement et al., 1998) and then purified using a PCR purification kit (PCR Clean-up Kit; PROMEGA Inc., Wisconsin, USA).
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bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was transferred to a new Eppendorf tube after being separated from Dynabeads using a magnetic separator (Promega, UK, Cat. CD4002).
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bioRxiv - Microbiology 2020Quote: ... Filters were transferred to 2 mL microfuge tubes for further processing using the Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation) with a modified version of the kit protocol ...
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bioRxiv - Microbiology 2023Quote: ... Two aliquots of 100 µL were transferred into new tubes and digested either with a sequencing-grade trypsin (Promega, Madison, WI, USA) or chymotrypsin (Promega ...
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Throat colour polymorphism in relation to sex and body size of the Litter skink, Lankascincus fallaxbioRxiv - Zoology 2022Quote: ... The PCR amplification was carried out in 25 μl volumes employing 35 cycles with an annealing temperature of 55 °C following standard PCR protocols with Promega PCR master mix (Promega Corporation, Madison, Wisconsin, USA). The success of the PCR amplification of each sample was confirmed through Gel electrophoresis using 5 μl of each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) after PCR amplification and extrication from the agarose gel using Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The threshold was set to 0.6 ΔRn ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were cloned using the pGEMT kit (Promega): 3 μl PCR products were ligated to 50 ng pGEMT vector in the presence of 3 units T4 ligase overnight at 18°C (final volume 10 μL) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated into pGEMT.easy (Promega, Madison, WI, US) and sequenced with Sanger sequencing ...
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bioRxiv - Molecular Biology 2020Quote: PCR reactions were carried out using Go-Taq enzyme (Promega) with 1 μl of cDNA diluted 1:5 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... purified chromatin was recovered using a PCR purification kit (Promega). Sequencing libraries were generated using standard methods and samples were sequenced on a MiniSeq instrument (Illumina ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were cloned into pGEM-T Easy vector (Promega) and sequenced using T7 promoter forward primer by Sanger sequencing ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were cloned into pGEM-T easy (Promega) and the sequence of the resulting plasmids was verified ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products cloning into pGEM-T-easy vector system (Promega). We Sanger sequenced 10 colonies per sample using M13 primers using the above described method and results were analysed with BISMA online tool (Bisulfite Sequencing DNA Methylation Analysis - http://services.ibc.uni-stuttgart.de/BDPC/BISMA/ ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed using GoTaq Green Master Mix (Promega), and gel analysis was performed in 1.5% agarose or 5 - 10% acrylamide gels ...
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bioRxiv - Genetics 2020Quote: ... The PCR is carried using GoTaq DNA polymerase (Promega, UK) and the following PCR conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were conducted using the GoTaq Master Mix (Promega Corporation), and products were run on a 1% agarose gel in 1X TAE Buffer.
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bioRxiv - Neuroscience 2021Quote: ... and PCR was performed using GoTaq green master mix (Promega). Primers used were ...
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bioRxiv - Plant Biology 2020Quote: ... or Wizard SV Gel and PCR Clean-Up System (Promega), and then sequenced.
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription (RT) involved the Access RT-PCR System (Promega), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... a PCR fragment was amplified using GoTaq DNA polymerase (Promega) and subcloned in a TOPO cloning vector (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed using GoTaq Green Master Mix (Promega) or Phusion High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were purified prior to quantification via QuantiFluor (Promega) and combined into an equimolar pool ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were cloned by A-tailing into pGEMTeasy (Promega) and sequenced ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was conducted using 1x Green GoTaq Flexi Buffer (Promega), 2.5 mM MgCl2 ...