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Citations for Promega :
101 - 150 of 3027 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: After PCR clean-up using Wizard SV Gel and PCR Clean-Up (Promega; A9281), samples were run in 2% agarose gel to ensure that the PCR product is compose of a single amplicon in the appropriate size ...
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bioRxiv - Genetics 2020Quote: ... The PCR was performed using the GoTaq® Long PCR MasterMix (Promega, Charbonnieres, France) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the PCR Promega kit (Promega Corporation, WI, États-Unis) following the manufacturer’s instructions and sent to Genewiz company (www.genewiz.com/en-GB ...
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bioRxiv - Microbiology 2023Quote: ... PCR samples were purified by the Wizard SV Gel and PCR Clean system (Promega) following manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR reaction was carried out using GoTaq Long PCR 2x Master Mix (Promega) for 35 cycles ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments from the nested PCR were cloned into pGEM-T-easy vectors (Promega), and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... 20 ml PCR reactions contained 10 ml GoTaq® green PCR Master mix (Promega), 6 ml nuclease-free water ...
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bioRxiv - Plant Biology 2024Quote: ... followed by PCR amplification across the CRISPR target sequences with DreamTaq PCR reagent (Promega) and Sanger sequencing (Eurofins genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 350 µL of this aqueous phase underwent automated extraction and cleaning using a Tecan Freedom EVO liquid handler with Promega Purefood protocol and reagents (cell lysis buffer, ReliaPrep™ resin, prepared wash buffer, isopropanol, 50% ethanol and Promega elution buffer). The total extracted and washed DNA was eluted in a final volume of 150 µL.
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bioRxiv - Microbiology 2020Quote: ... Afterwards 40 µL digestion buffer (1:10 diluted Rapid Digest buffer (Promega, Fitchburg, WI) in water ...
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bioRxiv - Microbiology 2022Quote: ... Then, 30μl of extension buffer (1X SSIII-RT buffer, 40U RNasin Ribonuclease Inhibitor (Promega) 2 mM DTT ...
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bioRxiv - Microbiology 2019Quote: PCR (GoTaq®Green, Promega) was performed on gDNA using primers listed in Suppl ...
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified (Promega) and digested using DpnI (NEB ...
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bioRxiv - Immunology 2019Quote: ... Access RT-PCR kit (Promega) was used for characterization of recombinant influenza viruses.
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bioRxiv - Neuroscience 2020Quote: ... PCR Nucleotide Mix (Promega, UK) and GoTaq® Flexi DNA Polymerase (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... PCR nucleotide mix (Promega: U144B) and oligo (dT ...
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bioRxiv - Genetics 2020Quote: ... PCR Master Mix 2x (Promega) was used ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR with GoTaq (Promega) using the primers-RG6 S ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10x T4 Ligase Buffer (Promega), T4 Ligase (2,000,000 units/mL ...
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bioRxiv - Neuroscience 2019Quote: ... in resuspension buffer (Promega, V542A) were prepared and subsequently diluted with ice-cold 50 mM AmBiC buffer to achieve a final concentration of 1 ng/µl ...
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bioRxiv - Genetics 2021Quote: ... in 1X Ligation Buffer (Promega) with 1% Triton X-100 and 10 μg/μl BSA and incubation at 16°C for 4 h ...
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bioRxiv - Systems Biology 2022Quote: ... Passive Lysis 5x buffer (Promega) was used and SAM (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 4 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL Greenflexi buffer (Promega), 2 µL dNTP (Promega U151B ...
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bioRxiv - Cancer Biology 2019Quote: ... using Reporter Lysis Buffer (Promega). Luciferase activity and total protein were measured as described previously [38] ...
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bioRxiv - Immunology 2019Quote: ... Lysis buffer (Promega UK Ltd.) was added to control samples and incubated for 45 minutes ...
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bioRxiv - Zoology 2019Quote: ... its reaction buffer (1X, Promega) and 10–100 ng of DNA template ...
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bioRxiv - Microbiology 2021Quote: ... 1x Fastbreak Lysis Buffer (Promega), 38 μg/mL lysozyme (Gold Bio) ...
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bioRxiv - Microbiology 2020Quote: ... 10x Pfu reaction buffer (Promega), Pfu DNA polymerase (Promega ...
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bioRxiv - Immunology 2021Quote: ... luciferase lysis buffer (Promega E1531) was added ...
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bioRxiv - Biochemistry 2022Quote: ... in resuspension buffer (Promega, V542A) and incubation on ice for 30 min and then at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... luciferase substrate buffer (SteadyGlo, Promega) was added for cell lysis and luminescence was detected as described above.
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bioRxiv - Genomics 2023Quote: ... 5X Passive Lysis Buffer (Promega) was diluted to 1X with PBS and 100 µL was added per well to harvest cell lysates ...
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bioRxiv - Developmental Biology 2023Quote: ... Reporter Lysis Buffer (Promega, #E1531) was employed.
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bioRxiv - Biochemistry 2023Quote: ... diluted in LCS buffer (Promega) was added to each well ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-90 real-time PCR System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using Wizard SV Gel and PCR Clean-Up System (Promega) according to the instructions by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned with the Wizard SV Gel and PCR Clean Up Kit (Promega) and cut with XhoI and BamHI or HindIII und BamHI (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated to the pGEM-T easy® vector (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega) and forward and reverse sequences were obtained using an Abi 3130XL Genetic Analyzer (Applied Biosystem ...
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bioRxiv - Microbiology 2019Quote: ... The semi-quantitative RT-PCR using 2X PCR Master Mix (Promega Corporation, Madison, WI, USA) and primers was carried out in a 20 μL reaction volume (1 μL cDNA ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega). The concentrations of amplified PCR products were measured using the Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes ...
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bioRxiv - Pathology 2021Quote: ... Quantitative RT-PCR was performed by using the Go-Taq Real-Time PCR system (Promega), as previously described60 ...
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bioRxiv - Developmental Biology 2019Quote: RT-PCR detection of Actn2 splicing variants was performed using GoTaq PCR master mix (Promega) and primers ...
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bioRxiv - Bioengineering 2021Quote: ... Input linear DNA was produced by PCR and cleaned with PCR cleanup kit (Promega, A6754), quantified and diluted to desired concentrations using ultra-pure water ...
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bioRxiv - Immunology 2023Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-90 real-time PCR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified with Wizard SV Gel and PCR Clean-Up System (Promega). A volume of 0.75 µl of the DNA templates was mixed in 2.5 µl cell-free protein expression reactions (PUREFrex 2.0 ...