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Citations for Promega :
51 - 100 of 4197 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... GRK371 and β-arrestin272 recruitment to chemokine receptors (CXCR1, CXCR2, CXCR3-A, CXCR4, CXCR5, CXCR6 and ACKR3) was monitored using a nanoluciferase complementation-based assay (NanoBiT, Promega)73,74 ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from strains containing extragenic suppressor mutations using the Wizard Genomic DNA Purification Kit (Promega) and sent for whole genome sequencing (Tufts University School of Medicine Genomics Core).
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bioRxiv - Microbiology 2021Quote: ... Cell viability was determined 24 h after the addition of compound-containing DMEM using the CellTiter-Glo kit (Promega) and a luminescence plate reader (Perkin Elmer) ...
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bioRxiv - Microbiology 2021Quote: ... containing 100ng/μL trypsin (Promega) or 100ng/μL GluC (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and dNTPs containing dUTP (Promega). A single 3’ “A” base was added to the end-repaired cDNA ...
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bioRxiv - Systems Biology 2023Quote: ... containing 0.5 µg Trypsin (Promega), 0.1 µg Lysyl Endopeptidase (FUJIFILM Wako ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 5mg/mL BSA(Promega) dissolved in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... containing RNase inhibitor RNasinTM (Promega). FACS analysis and sorting were performed on a (BD FACS Aria III ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 50mM DTT (Promega, #V3151), vortexed and heated up to 98°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Immunology 2020Quote: ... Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.
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bioRxiv - Immunology 2022Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega) at 450nm absorbance.
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bioRxiv - Microbiology 2019Quote: ... synthesized by in vitro transcription of a NdeI-linearized pGEM-T plasmid containing the 135bp cDNA target fragment using the T7 RiboMAX in vitro transcription kit (Promega). Negative controls (without template RNA and RNA from mock-infected cells ...
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bioRxiv - Synthetic Biology 2020Quote: Cells from HAC-containing clones have been harvested and genomic DNA has been collected using Maxwell DNA purification kit (Promega). qPCR analysis have been performed using SYBR Green Master Mix (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from mouse uterus and reverse transcribed into cDNA by reverse transcriptase kit (Promega). cDNA was amplified by gene specific primers ...
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bioRxiv - Cancer Biology 2020Quote: ... after isolating DNA from mouse tissue samples using a tissue DNA extraction kit (Maxwell® 16, Promega), the purified DNA was incubated and hydrolyzed enzymatically (EpiQuik ...
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bioRxiv - Genomics 2022Quote: ... DNA extraction from mouse liver tissue was performed using the Maxwell 16 Tissue DNA Purification kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor DNA was extracted from parts of FFPE containing a high fraction of tumor cells using Maxwell® RSC DNA FFPE Kit (Catalog number: AS1450, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing: Green 1X Go Taq (Promega) buffer ...
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bioRxiv - Cancer Biology 2024Quote: RNA from mouse tissue and from cell lines was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega) on the Maxwell RSC instrument according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Activation of chemokine receptors was detected by measuring ß-galactosidase activity using the commercially available Beta-Glo assay system (Promega Corp, Madison, WI, USA) and luminescence signal was recorded on a multimodal plate reader (Enspire 2300 ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Neuroscience 2021Quote: ... were transfected into monolayer culture cells with pGL4.29 containing CRE-luciferase reporter gene or pGL4.33 containing SRE-luciferase reporter gene (9.0 μg/dish, Promega) and pGL4.74 containing the Renilla luciferase reporter gene (0.5 μg/dish ...
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bioRxiv - Neuroscience 2021Quote: ... ELISA was performed on the homogenate using the BDNF Emax Immuno Assay Systems (Promega KK, Tokyo, Japan). The hippocampus (HPC ...
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bioRxiv - Immunology 2023Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega, Madison WI) at 450 nm absorbance.
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bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA was isolated from tail snip samples with Maxwell® Mouse Tail DNA Purification Kit (Promega, Madison, WI). Polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: The potential for M2e-specific antibodies to induce ADCC was evaluated using a mouse FcγRIV ADCC Reporter kit (Promega). In brief ...
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bioRxiv - Microbiology 2022Quote: ... containing 13.94 µl RNase free water (Promega), 6.25 µl master mix (Eurogentec) ...
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bioRxiv - Microbiology 2020Quote: ... in water) containing Rapid Digestion Trypsin (Promega) using a protein/enzyme (w/w ...
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bioRxiv - Bioengineering 2020Quote: ... containing 0.8 U/μL RNasin Plus (Promega) and GAPDH or KRT19 primers (primer sequences listed in Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
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bioRxiv - Biochemistry 2022Quote: ... containing 500 ng trypsin/LysC (Promega V5073) and digested for 16 hours at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... containing approximately 10 ng Trypsin Gold (Promega) and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... containing 4 μg/ml RNase A (Promega) for 10 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... containing 1 mM ethylenediaminetetraacetic acid (EDTA, Promega). The culture was then induced with 2 mM ZnCl2 and a 1 mL-sample was collected at 5 ...
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bioRxiv - Genetics 2019Quote: ... pRL-TK containing Renilla luciferase (Promega, USA), was simultaneously transfected into the cells with expression plasmids as an internal control for assay-to-assay variability ...
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bioRxiv - Neuroscience 2020Quote: ... containing a cocktail of protease inhibitors (Promega). Following solubilization by 1% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing a 2× concentration of Endurazine (Promega) was added to each well ...
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bioRxiv - Genomics 2023Quote: ... containing 1mM D-Luciferin Potassium Salt (Promega).
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bioRxiv - Cell Biology 2019Quote: ... ELISA data was normalized to viable cell number determined by MTT assay (Boehringer Ingelheim) or CellTiter-Blue (Promega). IFN levels were determined using a HEK293T IFN reporter cell line (clone 3C11 ...