Labshake search
Citations for Promega :
1 - 50 of 4197 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2 was fused in-frame upstream of the GBF1 truncated after HDS1 domain and containing a BFA-resistant Sec7 domain from ARNO (GARG-1060) in a mammalian expression vector pCI (Promega) generating a plasmid pCI-FLAG-APEX2-GARG- 1060 ...
-
bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
-
bioRxiv - Cell Biology 2022Quote: ... MYLK4 (MYLK4-NLuc) and TYK2(JH2 domain) (TYK2(JH2 domain)-NLuc) were kindly provided by Promega. NLuc (nanoluciferase ...
-
bioRxiv - Cancer Biology 2019Quote: FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
-
bioRxiv - Cancer Biology 2021Quote: All kinase assays for AURKA catalytic domain were performed with ADP-Glo™ Kinase Assay Kit (Promega©). For Alisertib the concentrations tested were ...
-
bioRxiv - Biochemistry 2022Quote: ... and M75F receptor plasmids using FuGene HD (Promega). Cells were treated with DMSO or varying drug concentrations 24 hours after transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... based on a mutated RIIB cAMP-binding domain from PKA (pGloSensor-20F, Promega), which produces rapid and reversible cAMP-dependent activation of luciferase activity in intact cells and is capable of detecting cAMP elevations in the absence of phosphodiesterase inhibitors ...
-
bioRxiv - Microbiology 2019Quote: ... containing 3ng of mouse GAPDH RNA (in-vitro transcribed using T7 RiboMAX (Promega), as per manufacturer’s protocol) ...
-
bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
-
bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
-
bioRxiv - Cell Biology 2019Quote: HepG2 cells or frozen mouse liver were sonicated in PBS containing protease and phosphatase inhibitors (Promega) in an ice-water bath using a Branson 250 digital sonifier programmed to cycle at a 10% amplitude with 5 seconds on followed by 20 seconds off ...
-
bioRxiv - Neuroscience 2019Quote: ... pDONR201 plasmid containing Ntrk2 was purified using a mini-prep kit (Promega) and recombined into pEF5/FRT/V5-DEST destination vector by LR clonase II reaction (ThermoScientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega. All other chemicals were purchased from readily available commercial sources and were used without further purification ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
-
bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
-
bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... A 9.5 kb fragment containing upstream promoter region of mouse Frizzled10 (Fzd10) gene was subcloned into pGEM vector (Promega). CreERT2gene (Feil et al. ...
-
bioRxiv - Immunology 2021Quote: ... DNA was extracted from mouse tail using genomic DNA extraction kit (Promega Wizard SV Genomic DNA Purification System ...
-
bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
-
bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
-
bioRxiv - Cell Biology 2020Quote: Identification of the β5-integrin carboxyl domain as Src substrate was determined using the ADP-Glo Kinase Assay (Promega, V6930) following protocols recommended by the manufacturer ...
-
bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... PGC-1α full-length and protein domain were translated in vitro in the T7 transcription and translation coupled reticulocyte system (Promega) supplemented with 35S-Methionine ...
-
bioRxiv - Physiology 2021Quote: ... A K13 donor template consisting of a 1.5 kb region of the K13 coding region including the entire propeller domain was amplified using the primer pair p3+p4 and cloned into the pGEM T-easy vector system (Promega). This donor sequence was subjected to site-directed mutagenesis in the pGEM vector to introduce silent binding-site mutations at the Cas9 cleavage site using the primer pair p5+p6 ...
-
bioRxiv - Biophysics 2023Quote: ... insertion of the FUS N-terminal domain) were synthesized and sequenced by the Genewiz Company from a pFN22A vector (Promega) containing the insert HaloTag-p53 WT ...
-
bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
-
bioRxiv - Developmental Biology 2021Quote: A partial TWD1 fragment of 150 bp covering the TPR domain and a part of the calmodulin binding domains (aa 264-314) was PCR amplified and cloned into pGEM-T-Easy Vector (Promega Corp.) and verified by sequencing ...
-
bioRxiv - Biophysics 2021Quote: ... and the twitchin kinase domain lacking flanking tails (Kin) was measured by monitoring ATP hydrolysis using the Kinase Glo™ luminescent assay system (Promega). Assays were performed using 10 µg of protein sample in a 50 µL reaction volume ...
-
bioRxiv - Biophysics 2019Quote: ... MDA-MB-231 cells were stably transfected with the plasmids coding for 3-phosphoinositide-specific Akt-PH domain fused with CFP and the plasmids coding for Rac1 fused with YFP using FuGENE® HD transfection reagents (Promega). MCF-7 cells and MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Cell Biology 2023Quote: ... ligation of double digested fragment of either domain with double digested pAcGFP1-N1 was performed with the LigaFAst Rapid DNA Ligation System (Promega, M8226) following the manufacturer’s suggested protocol ...
-
bioRxiv - Molecular Biology 2019Quote: ... using a coupled transcription-translation system containing wheat germ extract (TNT kit; Promega, Cat no. L5030), according to the manufacturer protocol.
-
bioRxiv - Bioengineering 2021Quote: PCR products containing the trigger T7 transcriptional unit were cleaned by PCR cleanup kit (Promega, A6754) and 250 ng were processed by in vitro transcription using the HiScribe T7 kit (Promega ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
-
bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
-
bioRxiv - Cell Biology 2020Quote: ... and the sequence encoding a genetically modified firefly luciferase into which a cAMP-binding domain has been inserted from the pGloSensor-20F (Promega, Cat #E1171).
-
bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
-
bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
-
bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
-
bioRxiv - Zoology 2021Quote: ... Those containing single bright bands were purified using Wizard SV Gel and PCR Clean-up Kit (Promega), and sequenced in both directions using PCR primers at Sequetech Inc ...
-
bioRxiv - Genetics 2023Quote: RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit (Promega). For the American vs standard diet comparison strand-specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol (Illumina ...
-
bioRxiv - Biophysics 2021Quote: ... sapiens TACAN-eGFP or the M2 muscarinic receptor (Halo-M2R-eGFP) plasmid (previously established in this lab) was transfected into the cells using FugeneHD (Promega) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...