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Citations for Promega :
701 - 750 of 1661 citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The amount of plasmid was determined using Picogreen DNA assay (#E2670, Promega, Madison, WI) following the manufacturers’ instructions.
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bioRxiv - Bioengineering 2021Quote: ... while the luciferase expression plasmid pGL4.50 was purchased from Promega (cat# E1310, Madison, WI).
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bioRxiv - Neuroscience 2021Quote: ... All described plasmids were transfected into cells using FuGENE® HD Transfection Reagent (Promega) according to the manufacturers protocol ...
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bioRxiv - Biophysics 2020Quote: ... mRNA was synthesized from these plasmids using a RiboMAX SP6 RNA production kit (Promega). In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA of insert-containing colonies was extracted (Wizard Plus SV Minipreps, Promega, France) and Sanger-sequenced in both directions by Eurofins Genomics (Germany) ...
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bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and a pcDNA5/FRT plasmid of interest using the Fugene 6 transfection kit (Promega) or Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 50 pg of the pGL4.23 luc2/miniP enhancer:luciferase plasmid and the following amounts of MOs or mRNAs into each dorsal marginal zone (dmz ...
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bioRxiv - Molecular Biology 2021Quote: The 3’ UTRs were cloned into psiCheck-2 plasmid (Promega, USA, Cat. No. C8021) using the XhoI and NotI sites ...
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bioRxiv - Neuroscience 2021Quote: Wildtype and miR-544-3p binding site-mutated 3’UTR of Fcgr1 were purchased from Thermo Fisher Scientific (Waltham, MA) and the inserts subcloned into the psiCHECK2 plasmid (Promega, Madison, WI) using GeneArt seamless cloning and assembly enzyme mix (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T TRIM21 KO cells were transfected with 2ug pGL4.32 NF-κB luciferase plasmid (Promega), using 12 uL of Viafect (Promega ...
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bioRxiv - Genomics 2022Quote: ... were transfected with 0.5ug luciferase constructs and 50ng pGL4.73 [hRluc/SV40] plasmid (Promega, E6911) containing Renilla luciferase ...
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bioRxiv - Developmental Biology 2022Quote: ... and/or pcDNA3.1-Flag-DN-xBrg1 plasmids using Fugen HD Transfection Reagents (2311; Promega), and after 48 h the transfected cells were lysed in NP-40 lysis buffer (P0013F ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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bioRxiv - Neuroscience 2019Quote: ... or mCherry along with (ii) pGlosensorTM -22F cAMP biosensor plasmid (Promega Corp., Madison, WI), which encodes a modified form of firefly luciferase with a fused cAMP binding moiety providing a biosensor for the direct detection of cAMP signalling in live cells ...
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bioRxiv - Biochemistry 2019Quote: ... and plasmids were extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). Plasmids were then treated with 5U DpnI (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... For the immunolabeling experiment HeLa cells were transfected with plasmids using FuGENE 6 (Promega). For streptavidin pull-down assays from HEK293T cells ...
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bioRxiv - Molecular Biology 2019Quote: ... The amount of DNA per well was equalized using mock plasmid DNA (pcDNA3.1, Promega). Forty-eight hours later ...
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bioRxiv - Genetics 2021Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: PCR fragments were cloned into the pGEM-T Easy plasmid (Promega Corporation, Madison WI) under the conditions established by the manufacturers and E ...
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bioRxiv - Biochemistry 2019Quote: ... and transfected with 0.5μg pCytERM-mAvicFP1 and pCytERM-mEGFP plasmid DNA using fuGENE (Promega) 24 hours prior to imaging ...
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bioRxiv - Immunology 2020Quote: ... Transfection with plasmid was performed following standard protocols for Fu gene 6 (Promega, #E2691). Cells were selected for stable expression using Geneticin (G418 sulfate ...
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bioRxiv - Microbiology 2019Quote: ... and was cloned downstream of the Renilla luciferase gene in the pSICHECK2 plasmid (Promega) by XhoI and NotI restriction sites ...
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bioRxiv - Genomics 2019Quote: ... The amplified sequence was inserted into nano-luciferase containing plasmid pNL3.2 (Promega, Madison, WI) using HindIII and XhoI (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 minutes before adding the transfection mix to the cells ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... together with pOG44 (Flp recombinase) plasmid using Fugene HD as per Promega protocol (Promega, Alexandria ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA was isolated using a Wizard Plus SV minipreps DNA purification kit (Promega) as per the manufacturers protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid constructs (pCAG nHA GPC4, see Table 1) were transfected with Fugene (Promega E2691)/ opti-MEM mixture according to manufacturer protocols ...
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bioRxiv - Genomics 2020Quote: ... pCGL-1-SNORD96A or empty pCGL-1 plasmid using Fugene (Promega, Madison, WI, USA). Cells were harvested 24- and 48-hours post-transfection for RNA isolation (TRIzol ...
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bioRxiv - Molecular Biology 2021Quote: ... and plasmids were purified using a Miniprep DNA purification kit (Promega Madison, WI, USA), followed by digestion with NdeI and XhoI (for gyrA)/ HindIII (for gyrB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with a total of 1μg combined DNA plasmids using FuGENEHD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µg of plasmid was mixed with 30 µL of FugeneHD (Promega, Cat# PAE2311) in Opti-MEM (Gibco ...
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bioRxiv - Biophysics 2022Quote: The pHaloTag-GR plasmid expresses rat GR fused to HaloTag (Promega, Madison, WI, USA) in the C-terminus regulated by a CMVd1 promoter and has been described previously (57) ...
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bioRxiv - Microbiology 2022Quote: Plasmid DNA was isolated using Qiagen Midi columns or Wizard mini prep columns (Promega). Specific DNA fragments were isolated using an agarose gel extraction kit (Roche Applied Science) ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg pΔ8.91 packaging and 16 µg lentiviral vector plasmid using Fugene (Promega, #E2312) according manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Plasmids encoding for NanoBiT system (SmBit and LgBit) were purchased from Promega (Madison, USA) and cloned upstream of the tau protein in pcDNA3.1 vectors by restriction enzymes ...
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bioRxiv - Genomics 2023Quote: ... These sequences were inserted into the hPGK promoter of the luciferase plasmid pGL4.53 (Promega) using Gibson assembly ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
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bioRxiv - Microbiology 2023Quote: ... and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Microbiology 2023Quote: The pOXA-48TET plasmid was extracted using the Wizard genomic DNA purification kit (Promega). The pUN4 and pBAD18 derivatives were isolated using the Plasmid Easypure isolation kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin free plasmid for transfection was purified using PureYield column kits (Mini/Maxi, Promega). Plasmid DNA for direct use in electroporation of iPSCs was further concentrated by precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, A1470) and validated by restriction digest and sequencing ...
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bioRxiv - Molecular Biology 2023Quote: HEK293-µOR cells were transfected with a plasmid encoding pGloSensor-20F cAMP reporter (Promega). Cells were harvested 24 h post transfection and resuspended at a 1.5x10^6 live cells/ml in assay media (DMEM without phenol red or FluoroBrite DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
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bioRxiv - Cell Biology 2023Quote: ... with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega; E2311) to produce lentivirus ...
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bioRxiv - Microbiology 2023Quote: ... and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The plasmid sequence was verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Genetics 2024Quote: ... transfected with 4 µg of plasmid DNA using the FuGENE® HD Transfection (Promega) for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: Transient plasmid WT-PolK GFP 54 transfections were performed using FuGene six reagent (Promega), according to manufacturer’s instructions ...