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Citations for Promega :
601 - 650 of 1661 citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... These fusions were expressed from plasmids based on the pGEM-T backbone (Promega) named pOP plasmids (22) ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmids were diluted in Opti-MEM and Fugene HD transfection reagent (Promega). The DNA-mixture was incubated for 30 min and then added drop-wise to the cells cultured in DMEM without penicillin/streptomycin for 6-7 h ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmids were diluted in Opti-MEM and Fugene HD transfection reagent (Promega). The DNA-mixture was incubated for 30 min and then added drop-wise to the cells cultured in DMEM without penicillin/streptomycin for 6-7 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and a Renilla luciferase reporter plasmid driven by a constitutive CMV promoter (Promega). 24 hours later ...
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bioRxiv - Microbiology 2022Quote: Plasmids (Table S2) used were constructed with DNA restriction nucleases (Promega – WI, USA), Gibson Assembly mix (New England Biolabs – MA ...
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bioRxiv - Immunology 2022Quote: ... Plasmids were transfected into cells with the aid of FugeneHD (Promega, Cat. E2311) using a ratio of 4 uL FugeneHD per 1 ug of plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: Transfection of plasmid DNA in HeLa cells was carried out using FuGENE (Promega). For the DNA transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid purifications were performed utilising Wizard Plus SV Minipreps DNA Purification Systems (Promega). New plasmids were tested by restriction enzyme digests and sequencing (Eurofins/GATC or Microsynth) ...
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bioRxiv - Microbiology 2020Quote: ... Fluc was amplified from pGL4.32[luc2P NF-kB-RE] plasmid (Promega, Madison, WI) and inserted into the vector backbone using Gibson Assembly ...
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bioRxiv - Genetics 2022Quote: ... HEK cells were transfected with 500 ng of plasmid using FuGENE 6 (Promega) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: DNA plasmid transfection were performed as previously described using FugeneHD (Promega, Madison, WI) 51 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... This genomic region had previously been cloned into the pGEM-T plasmid (Promega) and transformed into Escherichia coli(Ye et al ...
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bioRxiv - Neuroscience 2019Quote: ... and pGlosensorTM -22F cyclic adenosine monophosphate (cAMP) biosensor plasmid (Promega Corp., Madison, WI), which were used for receptor activation and intracellular signalling assays.
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA (6 μg) was delivered into U2OS cells using FuGENE 6 (Promega) and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The coding sequence of BclA3CTD was cloned in the plasmid pGEMT-easy (Promega) and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: Plasmids were transfected into U2OS cells using FuGENE® HD Transfection Reagent (Promega), according to the manufacture’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Oligos were annealed and inserted into the NotI/XhoI-digested psiCHECK2 plasmid (Promega). 1E5 cells/well were plated in 48-well plates and transfected with empty ...
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bioRxiv - Genomics 2019Quote: Linearized plasmid (2.5 ug) was added to a mixture containing transcription buffer (Promega), 10 mM DTT ...
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bioRxiv - Biophysics 2019Quote: ... The plasmids were transformed in Rosetta (DE3) and Rosetta (DE3) pLys cells (Promega). Cells were grown to an optical density of 0.7-0.8 in Luria-Bertani (LB ...
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bioRxiv - Genetics 2021Quote: ... Plasmid and template were co-transfected into LF2 mESCs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 0.02 ug of the pRL-CMV Renilla Luciferase plasmid (Promega Accession No. AF025843) was co-transfected with firefly luciferase plasmids ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual colonies were selected and isolated using the PureYield Plasmid Miniprep System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... where Firefly luciferase was substituted in the plasmid for NanoLuc luciferase (Promega, USA), transfected to HEK293 cells and IFNα-luciferase fusion proteins collected with tissue culture supernatant ...
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bioRxiv - Genomics 2019Quote: ... 400ng of the construct was transfected together with 40ng of Renilla plasmid (Promega) using lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... and target (400 ng) plasmids using 6 μL FuGene HD (Promega, Cat# E2311). 72 hrs later ...
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bioRxiv - Microbiology 2022Quote: ... The extracted plasmid was linearized with restriction enzyme BamHI (Promega, Madison, WI, USA), and thereafter purified with QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was purified using the Pure Yield Plasmid Midi Prep System (Promega, A2495).
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bioRxiv - Evolutionary Biology 2022Quote: Plasmid and B2 insert were ligated together using a rapid ligation kit (Promega). Ligation reactions contained 2.5µl 4x buffer ...
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bioRxiv - Microbiology 2022Quote: ... Replicon RNA was transcribed from linearized template plasmids with T7 RiboMAX Express (Promega) and cleaned by MEGAclear (Ambion/ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
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bioRxiv - Biophysics 2022Quote: ... transient transfection of the appropriate plasmid(s) was performed using FuGENE HD (Promega) according to the manufacturer’s procedure ...
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bioRxiv - Microbiology 2023Quote: ... The presence of the plasmid was confirmed by colony PCR (GoTaq polymerase, Promega). For construction of ATCC17978Δhcp::aprR ...
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bioRxiv - Biochemistry 2023Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
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bioRxiv - Genomics 2023Quote: ... The guide and donor plasmids were transfected into cells using Fugene HD (Promega). After seven days ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfections were performed by complexing plasmids with FuGENE® 6 Transfection Reagent (Promega) in OptiMEM media (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and pDEST26-Renilla plasmid (0.1 μg) using FuGENE® HD (Promega, Cat# E2312). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... The pRL-TK Renilla luciferase reporter plasmid was purchased from Promega (Madison, WI). E ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transfected into HEK293T cells using the ViaFect™ Transfection Reagent (Promega) according to manufacturer instructions 45.
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bioRxiv - Biochemistry 2023Quote: ... DNA used for transfection was purified using the PureYield Plasmid Midiprep System (Promega). After incubation for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid transfections were performed in MDA-T32 cell line using Fugene 6 (Promega). Transfected cells were cultured for over 3 weeks in complete RPMI-1640 medium containing G418 (600 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Biophysics 2023Quote: ... Purified DNA fragments were ligated into the pGEM®t-easy plasmid (Promega) and transformed into DH5α bacteria selected on standard Luria broth (LB ...
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bioRxiv - Genetics 2023Quote: ... or minimal promoter firefly luciferase reporter plasmid pGL4.26 [luc2/miniP/Hygro] (Promega, #E8441). Note ...
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bioRxiv - Microbiology 2023Quote: ... The firefly luciferase expression plasmid pGL3-CMV was purchased from Promega (Madison, WI).
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bioRxiv - Genomics 2023Quote: gBlocks with selected SNP-containing fragments (IDT) were cloned into pGL4.10 plasmid (Promega). For the luciferase reporter assay ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 µg of the specific lentiviral plasmid/construct using FugeneHD (Promega, E2311) in Optimem (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The next day cells were transfected with plasmid DNA using Viafect (E4982, Promega) at a ratio of 3:1 viafect to DNA ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... and envelop plasmid (VSVG) using FuGENE HD transfection reagent (Promega, Madison, WI, USA). The supernatant containing the constructed lentiviruses was collected after 24–48 h of transfection ...
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bioRxiv - Microbiology 2023Quote: ... generated by cloning the entire coding sequence of mouse APOBEC1 (mAPOBEC1) amplified from mouse cDNA by PCR into pGEM-T-easy (Promega), and cloning it into the EcoRI and SalI sites of pEGFP-C2 (Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-mouse HRP conjugate secondary (Promega W401B). Probing of Mcm2-7 using UM174 antibody was performed using 12% gels in order to compress signal from the six MCMs into a compact region to facilitate quantification ...