Labshake search
Citations for Promega :
101 - 150 of 4383 citations for Mouse B And T Lymphocyte Attenuator BTLA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: In vitro transcription and translation were realized from 500ng of cDNA pGEM-T construct using the TnT T7 Quick Coupled Transcription/Translation kit (Promega) and Transcend Biotin-Lysine-tRNA following the manufacturer protocol ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... PCR products were isolated with a QIAquick Gel Extraction kit (Quiagen) and purified fragments cloned into pGEM-T Easy vector (Promega). Specific details for the generated probes are as follow (gene name (accession number) ...
-
bioRxiv - Microbiology 2019Quote: ... synthesized by in vitro transcription of a NdeI-linearized pGEM-T plasmid containing the 135bp cDNA target fragment using the T7 RiboMAX in vitro transcription kit (Promega). Negative controls (without template RNA and RNA from mock-infected cells ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... An overhang was added to purified Or67b amplicons with Taq polymerase (Fermentas) and cloned using the pGEM-T Easy cloning kit protocol (Promega). Plasmids were extracted and purified using the GenElute plasmid miniprep kit (Sigma-Aldrich ...
-
bioRxiv - Immunology 2020Quote: The ability of T cells to lyse organoids was also measured using LDH-Glo™ Cytotoxicity Assay Kit (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
-
bioRxiv - Biophysics 2023Quote: ... The 12-230 kDa separation module was used for PCNA (CST, D3H8P, Rabbit, 1:100 dilution, #13110), b-Actin (CST, D6A8, Rabbit, 1:50 dilution, #8457S) and Halo (Promega, Mouse, 1:10 dilution, #G9211) detection ...
-
bioRxiv - Neuroscience 2019Quote: ... Then 5 μL of MTase reagent B (Promega) were added to all of the wells ...
-
bioRxiv - Cell Biology 2019Quote: ... 5’ GA CTC GAG GTT GCA CCT AGT GGA T 3’ The PCR product was first cloned into pGEM-T easy vector (Promega), verified by sequencing and subsequently cloned into pGEX5.1 vector using the EcoRI and XhoI sites ...
-
bioRxiv - Genetics 2020Quote: Via T/A ligation the fragment was inserted in the pGEM-T vector following the manufactures recommendations (Promega, Fitchburg, USA). Competent Escherichia coli cells (E ...
-
bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
-
bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40- pGEM-T Easy and MERS-CoV UpE-pGEM-T Easy were first linearized by Sal I enzyme (Promega) creating a 5’overhang ...
-
bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
-
bioRxiv - Plant Biology 2019Quote: ... cloned into pGEM®-T easy vector (Promega). Five clones for each variety were sequenced and aligned with Fragaria x ananassa cv ...
-
bioRxiv - Genetics 2021Quote: ... cloned into the pGEM-T Easy vector (Promega), and finally sequenced (SEQme ...
-
bioRxiv - Physiology 2019Quote: ... or PGEM-T Easy Vector (Promega, Wisconsin, USA) using primers shown in Table 1 and cDNA from medaka brain and pituitary total RNA ...
-
bioRxiv - Microbiology 2019Quote: ... Constructs were cloned into pGEM-T easy (Promega) or pJET1.2/blunt (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Escherichia coli DH5α and pGEM-T easy (Promega) and pJET1.2/blunt (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... which was ligated into pGEM-T easy (Promega) and sequenced to ensure proper splicing ...
-
bioRxiv - Plant Biology 2021Quote: ... A pGEM-T Easy vector (Promega, Madison, USA) cloned with a 450-bp fragment from SCYLV ORF3 previously amplified by RT-PCR was used to construct a serial dilution curve with six points and tenfold dilutions between points ...
-
bioRxiv - Plant Biology 2021Quote: ... USA),cloned in pGEM-T easy vector (Promega, USA), and at least five individual clones were sequenced for each target.
-
bioRxiv - Plant Biology 2020Quote: ... The amplificate was ligated into pGEM-T (Promega), from which the gene was excised with XhoI/EcoRI and ligated into pBAD/HisA (Invitrogen) ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
-
bioRxiv - Biochemistry 2022Quote: ... The product was cloned into pGEM-T (Promega) and the resulting rbs_csp3 fragment sub-cloned into pDR111 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... or pGEM®-T Easy (Promega, Madison, WI) followed by sequencing of plasmids isolated from 10-15 colonies ...
-
bioRxiv - Neuroscience 2021Quote: ... or pGEM®-T Easy Vector System (Promega). Nucleotide sequences of primers were summarized in Table 1 (No ...
-
bioRxiv - Microbiology 2021Quote: ... ligated into the pGEM T Easy vector (Promega) and transformed in DH10B chemo-competent cells ...
-
bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
-
bioRxiv - Molecular Biology 2019Quote: ... cloned into the pGEM T-Easy vector (Promega; according to the manufacturer’s instruction ...
-
bioRxiv - Epidemiology 2019Quote: ... and cloned into pGEM-T vector (Promega, USA). In total six 16S rDNA and two rpoB clones were sequenced and analysed using the BLASTN algorithm and finally aligned using Spiroplasma glossinidia data from NCBI as reference.
-
bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
-
bioRxiv - Molecular Biology 2020Quote: ... and cloned in pGEM-T easy vector (Promega); Four to six positive clones were sequenced (MWG ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... cloned into pGEM-T Easy vectors (Promega, USA) and then transformed into competent Escherichia coli cells for amplification ...
-
bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
-
bioRxiv - Developmental Biology 2019Quote: ... and inserting it into pGEM-T Easy (Promega) by TA cloning ...
-
bioRxiv - Cell Biology 2021Quote: ... Self-ligated empty pGEM-T vector (Promega, USA) was used to ensure equal amounts of transfected DNA ...
-
bioRxiv - Cancer Biology 2021Quote: ... cloned into pGEM®-T Easy Vector (Promega), and then into the pSec-Tag2/hygroA vector (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... and TA cloned into pGEM-T Easy (Promega) then transformed into DH5α competent E ...
-
bioRxiv - Microbiology 2020Quote: ... Splice2 was cloned into pGem-T Easy (Promega) and integrity of the DNA confirmed by sequencing ...
-
bioRxiv - Microbiology 2022Quote: ... cloned into the pGEM-T easy plasmid (Promega), and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the promoterless lacZ gene ...
-
bioRxiv - Microbiology 2023Quote: ... The pGEM-T Easy Vector (Promega, Madison, USA) and pMG36e vector (Addgene ...
-
bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T Easy empty vector (Promega) and sequenced ...
-
bioRxiv - Microbiology 2023Quote: ... The amplicon cloned into pGEM T-EASY (Promega). An 804 bp portion of the hsdM1 coding sequence was deleted by inverse PCR with the inclusion of a unique BamHI site in each of the following primers ...
-
bioRxiv - Molecular Biology 2023Quote: ... and cloned into pGEM-T Easy Vector (Promega). Positive clones were identified by the blue/white screening method and sequenced on ABI PRISM 3100 automated sequencer at Eurofins Genomics Europe Sequencing GmbH.
-
bioRxiv - Molecular Biology 2023Quote: The pGEM®-T Easy Vector System (Promega) was used according to the manufacturer protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were cloned in pGEM-T (Promega), and the inserts were sequenced by Sanger sequencing (University of California Berkeley DNA Sequencing Facility) ...
-
bioRxiv - Microbiology 2024Quote: ... ligated into the linearized vector pGEM-T (Promega) and transformed via electroporation into E ...
-
bioRxiv - Immunology 2021Quote: MSCs and B cells proliferation were measured using the CellTiter 96® AQueous cell proliferation assay kit from Promega (Madison, WI) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...