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Citations for Promega :
1 - 50 of 4383 citations for Mouse B And T Lymphocyte Attenuator BTLA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... B lymphocyte proliferation was measured using the cell proliferation assay kit (CellTiter 96 Aqueous) from Promega (Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti b-galactosidase (1:500; Promega # Z3781), chicken anti b-galactosidase (1:500 ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or a pGEM®-T Easy Vector (pGEM®-T Easy Vector Systems Kit, Promega, USA) depending on the Taq-polymerase used for PCR (blunt vs ...
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bioRxiv - Biochemistry 2020Quote: ... The pGEM-T Vector kit was purchased from Promega. Acrylamide/bisacrylamide (29:1 ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed with TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse IgG (diluted 1:1,000 in TBS-T) (Promega, Madison, WI, USA) for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: The T Cell Activation Bioassay kit was purchased from Promega. The Jurkat-NFAT cells were thawed and cultured in RPMI (supplemented with 10% FBS and 5% pen/strep ...
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the pGEM T-Easy vector kit (Promega). Clones were fully sequenced and confirmed as the targeted sequence ...
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bioRxiv - Microbiology 2021Quote: ... and pGEM®-T vector kit (Promega, Madison, Wisconsin, USA), respectively ...
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bioRxiv - Pathology 2021Quote: ... The DNA-A and DNA-B fragments were purified and cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) and then transformed into Escherichia coli strain DH5α cells by the heat shock method ...
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bioRxiv - Microbiology 2023Quote: ... generated by cloning the entire coding sequence of mouse APOBEC1 (mAPOBEC1) amplified from mouse cDNA by PCR into pGEM-T-easy (Promega), and cloning it into the EcoRI and SalI sites of pEGFP-C2 (Clontech) ...
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bioRxiv - Developmental Biology 2022Quote: ... or pGEM vector using pGEM-T easy vector system kit (Promega). Inserted sequences were excised by digestion with ClaI and inserted into the ClaI site of the replication-competent retroviral vector RCASBP(A) ...
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bioRxiv - Molecular Biology 2022Quote: ... Bands <2kb were cloned using the pGEM-T Easy kit (Promega) and bands >2kb were cloned using the Topo-XL2 PCR kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... We used the pGEM® T-Easy Vector System kit (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... 7 and 10 were either cloned with the pGEM®-T kit (Promega) to assay individual sequences and/or sequenced by Illumina (Centre d’expertises et de services Genome Québec) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or into the pGEM-T or pGEM-T Easy vector (Promega) and subsequently sequenced (Macrogen).
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bioRxiv - Plant Biology 2022Quote: ... The amplicons were subcloned using pGEM-T Easy Vector Systems Kit (Promega, Tokyo, Japan) or Zero Blunt PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Each product was cloned using the pGEM®-T Easy vector kit (Promega Corp.) to derive 12 clones using the GeneJet plasmid purification kit (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... b-gal (Promega, Z3781), p21 (BD Pharmingen ...
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...
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bioRxiv - Genetics 2020Quote: ... then cloned using the pGEM T-Easy kit and manufacturer’s protocol (Promega, Madison, WI, USA). pGEM vector containing the desired PCR product was transformed into competent JM109 E.coli cells (Promega ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Cancer Biology 2021Quote: T Cell Activation Bioassay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... The pGEM T-easy (Promega) based positive control plasmid of NNV was constructed from a 605 bp RNA2 fragment amplified from NNV-infected orange-spotted grouper (laboratory sample) ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy (Promega) were used ...
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bioRxiv - Microbiology 2022Quote: ... pGEM-T Easy vector (Promega) (supplementary data table 6) ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into pGEM-T (Promega) and used to synthesize digoxigenin-labeled probes by in vitro transcription using SP6 or T7 polymerases (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Genomics 2019Quote: ... which were subsequently ligated to pGEM-T Vector (pGEM-T Vector System I, Promega, USA), and transformed to DH5α competent cells (Z-Competent E ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-T-Cofilin 1:2000 (Bamburg lab, 1439 or Cell Signalling, 5175) and mouse anti-β3-tubulin 1:2000 (Promega, G7121) diluted in 1%BSA/PBS and incubated overnight at 4ºC ...
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bioRxiv - Biochemistry 2020Quote: ... three washes of 10 min in PBS-T were performed and membranes were incubated 1 hour at RT with the following 1:5000 or 1:2500 horseradish peroxidase conjugated secondary antibodies in 1% milk in PBS-T: anti-mouse (Promega, #W402B), anti-rabbit (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using T3 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using SP6 RNA polymerase and the DIG-labelling kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... and resulting PCR products were subcloned into a pGEM-T vector (Promega, pGEM-T Vector Systems) for subsequent Sanger sequencing of both alleles ...
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bioRxiv - Immunology 2021Quote: ... or pGEM-T plasmid (Promega, UK), manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2021Quote: ... serial dilutions of pGEM-T (Promega) plasmids containing coding sequences of the target genes or internal control genes (rp49 for Drosophila melanogaster and AeRpL32 (AAEL003396 ...
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bioRxiv - Plant Biology 2020Quote: ... cloned in pGEM-t Easy (Promega), re-amplified by PCR with primers with attB tails and BP-cloned into pDONR207 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The pGEM-T donor vector (Promega) was engineered by introducing a donor construct ...
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bioRxiv - Molecular Biology 2020Quote: ... subcloned via pGEM-T easy (Promega) and inserted upstream of a chloramphenicol resistance cassette in the plasmid pSF36 (pUC19+cm-FRT-HindIII ...
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bioRxiv - Microbiology 2019Quote: ... cloned into pGEM-T Easy (Promega), and then inserted using the NotI site ...
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bioRxiv - Microbiology 2020Quote: ... ligated to pGEM-T Easy (Promega), and transformed into E ...