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Citations for Promega :
501 - 550 of 866 citations for Mouse Anti Nipah Virus Glycoprotein G CF12 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: The 1-450bp DNA region of the luciferase coding sequence was synthesized with G/C content optimized and ‘ATG’ codons removed and then inserted into the pGL3-Promoter vector (E1761, Promega) in place of the original full-length luciferase coding region using ClonExpress®II One Step Cloning Kit (C112-01 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μg of DNase-treated total RNA was applied for cDNA synthesis using an Improm-II Reverse transcription system (A3802, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... were transfected at 80-90% confluence with lentivirus helper plasmids pVSV-G and psPAX2 in along with the constructed lentiviral transfer vector using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and a non-target control (NT-Ctrl) along with the packaging plasmids (pHCMV-G, and pHCMV-HIV-1) (44) using FUGENE-HD transfection reagent (Promega). The mouse Drp1-targeted shRNA plasmid with the sense sequence of GGCAATTGAGCTAGCTATA ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM MgCl2, 1.5 mM KCl, 100 μ g/ml cycloheximide, 1mM DTT, 200 U/ml RNase in from Promega, 0.5% Sodiumdeoxycholate ...
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bioRxiv - Cancer Biology 2022Quote: The cell viability was measured by MTS assay according to the manufacturer’s protocol (Cat. No. G-111, Promega, Madison, WI) and as described previously16,26 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... and the lentiviral packaging plasmids pVSV-G, pDVPR, and pAdvant (gifts from Jan Carette, Stanford University) using FuGENE HD transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
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bioRxiv - Molecular Biology 2023Quote: ... together with pCG-gag-pol and pCG-VSV-G (a gift from Dr. T. Yasui, National Institutes of Biomedical Innovation, Health and Nutrition) using FuGENE HD (E2311; Promega) for 4 h in Opti-MEM (31985-070 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... A pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with the pHR transgene expression vector and viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). At 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... VSV-G agarose beads and bound proteins were then treated with 10 μL of 10 μg/μL sequence grade trypsin (Promega) for 16 h at 37℃ with mild shaking ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated in HEK293T cells by transfecting lentiviral transfer vector, and helper vectors (psPAX2, and pVSV-G) using Fugene HD (Promega). Lentiviral supernatant was collected 48 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were then plated onto 35 mm glass coverslips pre-coated with poly-D-lysine (100 g/mL) and transfected with 300 ng of each plasmid using FuGene transfection reagent (Promega) or Lipofectamine 2000 (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Microbiology 2020Quote: ... Secondary -mouse IgG-HRP conjugate (Promega, Mannheim, Germany; 1:4,000 dilution) was used for detection ...
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bioRxiv - Biochemistry 2022Quote: ... The SNRPN promoter was amplified from mouse genomic DNA (Promega, G3091) using design primers (Supplementary Table 3) ...
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bioRxiv - Developmental Biology 2022Quote: ... from mouse genomic DNA and PCR products cloned into pGL4.23 (Promega) pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... coated dishes with 30 ng/ml of mouse NGF (G5141, Promega). Then the neurons were microinjected with METTL3/14 activators (200 nl ...
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bioRxiv - Microbiology 2024Quote: ... Mouse antibodies (CV801, CV804, CV820, CV925, CV1117) and effector cells (Promega) expressing mouse FcγRIV and NFAT luciferase reporter were added to the cells ...
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bioRxiv - Plant Biology 2019Quote: ... An aliquot containing 4 μg RNA was treated for 45 min with 2 μl of DNase RQ1 (1 μg/μl) at 37 °C (Promega, UK), and purified using an RNAeasy spin column (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The LCLs were further screened for karyotype abnormalities using G- banding approach and sample identity confirmation was done by STR profiling [GenePrint® 10 System (Promega)].
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bioRxiv - Cell Biology 2021Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were reverse-transfected at the day of seeding with 17µg of plasmid either coding for eGFP-RNF213 or eGFP-RNF213-ΔC using 51 µL of Fugene® HD (#E2311, Promega) for a ratio of 1:3 (µg cDNA / µL transfection reagent) ...
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bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: 5’-LTR of HERV-R was cloned in fusion with the downstream Gaussia Luciferase (G-Luc) into the pGL3 basic vector (Promega, USA). The sequence corresponding to 596 nucleotides of HERV-R 5’-LTR (22061-22657 base pairs on human chromosome 7q11 ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg linearized vector was used for in vitro transcription using the T7 RiboMAX™Express Large-Scale RNA Production System (Promega) according to the manual ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Synthetic Biology 2023Quote: ... with a pLV-EF1a-IRES or pTwist-SFFV transgene expression vector and the viral packaging plasmids psPAX2 and pMD2.G using Fugene HD (Promega #E2312). Viral production was performed in 6-well tissue culture treated plates (Corning 3335) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ARC (SantaCruz Biotechnology, #sc-17839, anti-HaloTag (Promega, #G9211), anti-SNAP-tag (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection® Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were packaged with the third-generation lentiviral packaging system (VSV-G, pMDLg, and pRSV-REV) and expressed in HEK293T cells via transient transfection with FuGENE 6 (Promega, Madison, WI). Virus-containing supernatants were harvested after 24 and 48 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrates were washed thrice with 50 mM PB by centrifugation at 16 000 g and digested with sequencing grade Trypsin-Gold (Promega, Madison, USA) at the ratio of 1:30 (enzyme/protein ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 μg/well of either pGL3-B.HTSS2KB or pGL3-Intron3-DSCR1 reporter construct was transfected with 150 μg/well of pCDNA3-ΔCnA,and 300 μg/well pEF-BOS-hNFATp and/or 150 until 600 μg/well of pEGFP-VIVIT.7,17 pRL-TK (Promega; xx μg/well), containing the thymidine kinase promoter driving Renilla luciferase ...
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bioRxiv - Immunology 2024Quote: ... 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega, cat. E2691) and OPTI-MEM (GIBCO ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or HA-mouse μOR and pGloSensor22F-cAMP plasmids (Promega, Madison, WI, USA) using Xtremegene9 (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from mouse tail using genomic DNA extraction kit (Promega Wizard SV Genomic DNA Purification System ...
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bioRxiv - Molecular Biology 2022Quote: ... Both mouse brain and cell lysates were digested with DNase I (Promega) for 5 minutes at 37°C and then with RNase A (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Goat immunoglobulins against rabbit or mouse immunoglobulins conjugated with horseradish peroxidase (Promega) were used as secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded into 24-well plate and cultured to 80% confluence when they were co-transfected with 4 μg of either TOP flash or FOP flash vector and 1 μg of pRL-TK (Renilla-TK-luciferase vector, Promega, Madison, WI, USA) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 µg retroviral vectors were independently introduced into Plat-GP cells using 2.25 µL of FuGENE 6 transfection reagent (Promega, Madison, WI, United States). In addition ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of pPAX packaging vector and 1 μg of VSV-G envelope vector using FuGENE® HD Transfection Reagent (Promega, cat.no. E2311), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and the goat anti-rabbit antibody (anti-rabbit IgG AP conjugate, Promega) as secondary antibody (30) ...
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bioRxiv - Plant Biology 2020Quote: ... an immunoblot with anti-halo antibody (Anti-HaloTag® Monoclonal Antibody-Promega) was performed to confirm protein expression and binding efficiency for each TF ...