Labshake search
Citations for Promega :
451 - 500 of 866 citations for Mouse Anti Nipah Virus Glycoprotein G CF12 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... mouse monoclonal antibIII tubulin (1:500, Promega, #G712A), mouse anti-tubulin bIII (1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: ... Cell signalling) or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
-
Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... The pellets from centrifugation at 1,500 g for 3 min were lysed in Reporter lysis buffer (Promega) containing 0.16 U µl−1 Ribosafe RNase inhibitors ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1500 μg total protein lysate was 100ul slurry of pre-cleared with Protein G agarose beads (Promega), followed by overnight incubation with AXL antibody (2ug Ab/ 100 ul lysate) ...
-
bioRxiv - Biophysics 2022Quote: ... EGFP-EGFP-EGFP-N1 or VsV-G-EGFP 12-16 hours before the experiment using Fugene6 (Promega). CHO cells stably expressing GFP-GPI were obtained earlier (Sharma et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... pLKO shRNA-DNA was transfected with psPAX2 packaging and pMD2.G envelop plasmid using Fugene reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91(1.33μg) using 10μl of Fugene HD (Promega #E2312) according to manufacturer protocols ...
-
bioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using FuGENE HD (Promega, #E2312). Primary T cells were thawed the same day and after 24 h in culture ...
-
bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were collected by centrifugation at 8000 × g for 30 s or using a magnet separator (Promega), and washed three times with lysis buffer ...
-
bioRxiv - Synthetic Biology 2024Quote: Pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with a modified pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). Primary T cells were thawed the same day ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91 (1.33μg) using 10ul of Fugene HD (Promega #E2312), according to manufacturer procedure ...
-
bioRxiv - Neuroscience 2020Quote: ... US) and TubβIII (mouse, 1:1000, G712A, Promega, UK). Slides were then incubated with the appropriate fluorophore-conjugated secondary antibodies ...
-
bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
-
bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
-
bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... collected at 2000 x g followed by resuspension in 0.5 ml of storage buffer (PBS containing 4% BSA and RNasin (Promega) – 0.2 U/ul ...
-
bioRxiv - Immunology 2019Quote: ... 0.4 µg envelope vector (pMD2.G) and 1.6 µg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
-
bioRxiv - Microbiology 2021Quote: ... and the lentiviral packaging plasmids pVSV-G, pΔVPR, pAdvant (gifts from Dr. Jan Carette, Stanford University) using FuGENE HD transfection reagent (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... Lentivirus was produced by transfecting HEK293T cells at 80% confluence with a mixture of the transfer plasmid pLX302-Igk-FLAG-Subtiligase-PDGF TM and the second-generation lentiviral packaging plasmids (pMD2.g and pCMV-R8.91) using FuGENE HD transfection reagent (Promega). After 6 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... Release of LDH was assessed by LDH CytoTox 96® Non-Radioactive Cytotoxicity Assay (G 1780, Promega, WI, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... the lentivirus was produced by transfecting 70% confluent HEK 293T cells with the desired plasmid along with psPAX2 and pMD2.G following the manufacturer’s instructions (FuGENE HD, Promega). The virus-containing medium was collected 48 h after transfection ...
-
bioRxiv - Immunology 2022Quote: ... the lentivirus was produced by transfecting 70% confluent HEK 293T cells with the desired plasmid along with psPAX2 and pMD2.G following the manufacturer’s instructions (FuGENE HD, Promega). The virus-containing medium was collected 48 h after transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Denaturated RNA (5μg) was retrotranscribed and used for quantitative PCR using GoTaq qPCR Master Mix according to the manufacturer’s instructions (Promega) at a dilution of 1/10 for mycelium and 1/7 for rice leaves ...
-
bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were diluted 3 fold with 20mM HEPES pH 8.0 and digested in 10 @g ml-1 trypsin-TPCK (Promega) overnight at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Transfection of the different plasmids was made in a ratio 4:1:4 using lentiviral components pCMVR8,74 (Gag-Pol-Hiv1) and pMDG2 (VSV-G) vectors and the plasmid of interest respectively using FuGENE HD (Promega) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 10,000 x g and 4°C for 2 min and digested by addition of RNAse-free DNAse I (Promega) for 10 min on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... Total RNA (1-2 μg) was reverse transcribed with a GoScriptTM Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation ...
-
bioRxiv - Cell Biology 2024Quote: 1μg of each plasmid or pcDNA3.1 empty vector was reverse transfected into 1x106 HEK293T cells using FUGENE HD (Promega) as per manufactures instructions ...
-
bioRxiv - Microbiology 2022Quote: ... As secondary antibodies α-mouse IgG HRP conjugate (Promega W4021) or α-rabbit IgG HRP conjugate (Cell Signaling #7074 ...
-
bioRxiv - Cancer Biology 2022Quote: ... following intraperitoneal injection of luciferin substrate (3mg/mouse; P1043 Promega). Leukemia burden in the peripheral blood was monitored twice weekly by flow cytometric analysis of hCD45+ proportions ...
-
bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit ...
-
bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies where HRP conjugated (Promega, Rabbit W401B, Mouse W402B) for ECL detection using Pierce ECL2 solution (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... and HaloTag (mouse monoclonal, Promega no. G9211; 1:1000 dilution). Membranes were incubated for one hour at room temperature with IRDye secondary antibodies (Goat anti-Mouse 680RD ...
-
bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated secondary antibodies against mouse and rabbit IgG (Promega).
-
bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated mouse secondary antibody (Promega). UAP56/DDX39B [1:2000] (custom generated51 ...
-
bioRxiv - Cell Biology 2023Quote: Mouse livers were homogenized in Luciferase Culture Lysis Reagent (Promega). Lysates were clarified by centrifugation at 13,000 × g for 20 min at 4 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Equal amounts of RNA (1 μg) were reverse-transcribed with a mixture of oligo(dT)15 and random hexamer primers using M-MLV polymerase (Promega). RT-qPCR gene expression quantifications were performed using AceQ qPCR SYBR Green Mix (Vazyme Biotech ...
-
bioRxiv - Microbiology 2021Quote: ... 2 μg of each env clone was co-transfected with 4 μg of pNL4.3-ΔEnv-vpr+-luc+ into 293T cells in a T25 flask using the FuGENE6 transfection reagent (Promega, USA). The culture supernatants containing the pseudoviruses were harvested at 72 hours post transfection ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.25 µg of DNA was used to amplify specific regions of Trp53 and Nf1 gene using Go-Taq DNA Polymerase (Promega). The primers sequences are summarized in Suppl ...
-
bioRxiv - Bioengineering 2020Quote: VSV-G-pseudotyped lentiviral vectors were produced by co-transfecting HEK293T cells with lentiviral backbone plasmids and packaging plasmids (pHDM-Hgpm2, pHDM-tat1b, pRC/CMV-rev1b, pHDM-G) using Fugene HD (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
-
bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega, WI, USA). Supernatants containing virus were collected at 48h and 72h after transfection ...
-
bioRxiv - Neuroscience 2021Quote: ... The total RNA (1-2 μg) was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation ...
-
bioRxiv - Plant Biology 2022Quote: ... and 5 μg plasmid DNA was co-transfected with same amount of low-rate constitutively expressed pRL-TK Renilla luciferase vector (Promega) into mesophyll protoplasts isolated from A ...
-
bioRxiv - Molecular Biology 2022Quote: ... Retroviral supernatants were obtained from 293GP cells co-transfected with pVSV-G and pBABE-puro-hTERT or pBABE-hygro-KRAS-V12 constructs (kind gifts from Jerry Shay) using FuGENE HD (Promega) and passed through a 0.45 um filter ...
-
bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...