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Citations for Promega :
151 - 200 of 1814 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with control (mCherry) or Cre-T2A-mCherry-containing pFUGW vector plasmids and helper plasmids PAX2 and VSVG using Fugene6 (Promega, Cat #E2691). Supernatant was collected 65 hr after transfection and filtered through a 0.45 μm filter (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Immunology 2021Quote: ... M-MLV (Promega, M1701) in the presence of RNase inhibitor (Promega ...
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bioRxiv - Microbiology 2023Quote: ... M-MLV (Promega, #M1701); Random primers (Invitrogen™ ...
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bioRxiv - Biochemistry 2023Quote: M-MLV buffer (Promega), 1 μL 0.1M DTT ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry and CLIP were PCR amplified from pFN21A (Promega), pSNAPf (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... The coding sequence of BclA3CTD was cloned in the plasmid pGEMT-easy (Promega) and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... and SYBR Green fluorescent dye (Promega) were used ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To detect fluorescent signal Glowmax (Promega) instrument with excitation and emission filters of 360 nm and 460 nm ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were diluted 8x in 50 mM ammonium bicarbonate to reduce the urea concentration to 1 M and protein digestion was performed overnight at 37 C by addition of 2 µg of trypsin (Promega) per sample.
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... 24h prior to imaging cells were co-transfected with human PEX31-42-GFP-Halo and either human HAP1-mCherry-eDHFR or mouse BICD21-572-mCherry-eDHFR using FuGENE 6 (Promega; 1 µg total DNA) and 48h prior to imaging with control siRNA using Lipofectamine RNAiMAX (Thermo Fisher) ...
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bioRxiv - Plant Biology 2023Quote: The coding sequences of BdNRT2A and BdNRT3.2 were cloned into pGEMT easy vector (Promega) and then digested with NotI enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... The NanoLuc coding sequence including linker was obtained from the pNLF1-C plasmid (Promega) and inserted at the C-terminus of the TDO2 gene sequence (Flash Therapeutics ...
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bioRxiv - Neuroscience 2020Quote: ... 10.67 % m/v PEG8000 (Promega), titrated amount of home-made Tn5 enzyme) ...
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bioRxiv - Plant Biology 2021Quote: ... M-MLV Reverse Transcriptase (Promega) was used for reverse transcription on two μg of DNA-free RNA.
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bioRxiv - Physiology 2020Quote: ... M-MLV Reverse Transcriptase (Promega) and a set of random hexamers were used with 1 μg total RNA following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercially available cell-impermeable fluorescent ligands were utilized: HaloTag Fluorescent Ligand Alexa Fluor 488 (Promega, #G1002) in the following abbreviated as CA-Alexa488 ...
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bioRxiv - Biophysics 2023Quote: ... Cross-linked samples for XL-MS were digested by 1:50 (m/m) trypsin (Promega) overnight at 37 °C while shaking at 600 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... the coding region of a modified firefly luciferase gene (LUC+, Promega Corp, Madison, WI, USA), and TNOS ...
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bioRxiv - Cancer Biology 2021Quote: The coding sequence of the mature IL-18 was cloned into pET-20b plasmid (Promega) before being transduced into E ...
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bioRxiv - Plant Biology 2021Quote: Full length Luciferase coding sequence was PCR amplified from the pGEM-luc cassette vector (Promega) using the primer pair ...
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bioRxiv - Molecular Biology 2022Quote: The coding regions for the firefly luciferase (from the vector pGL4.51[luc2/CMV/Neo] (Promega)) or the camelid anti-BoNT/A sdAb (clone B11 ...
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bioRxiv - Genomics 2023Quote: ... the HaloTag coding sequence was amplified from the pHTC HaloTag CMV-neo Vector (Promega #G7711). Cloning reactions were transfected into NEB stable competent E ...
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bioRxiv - Neuroscience 2021Quote: ... and M-MLV Reverse transcriptase (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and M-MLV reverse transcriptase (Promega). Real-time PCR was carried out using Sybr Green Master Mix (A&A Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... and M-MLV Reverse Transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The M-MLV reverse transcriptase (Promega) was used for cDNA preparation ...
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bioRxiv - Microbiology 2020Quote: ... M-MLV reverse transcriptase (#M368A, Promega) was used to reverse transcribe viral RNA with the reverse PCR primer according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and M-MLV reverse transcriptase (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... M-MLV reverse transcriptase (#M368A, Promega) was used to reversely transcribe viral RNA with the reverse PCR primer according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and M-MLV Reverse Transcriptase (Promega). Primers were allowed to bind to the template RNA for 5 min at 70oC ...
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bioRxiv - Cell Biology 2022Quote: ... and M-MLV reverse transcriptase (Promega). qPCR analysis was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and M-MLV reverse transcriptase (Promega) were used ...
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bioRxiv - Cell Biology 2022Quote: ... 80 µl 0.1 M IPTG (Promega) were added to the bacterial lawn to induce dsRNA expression before eggs (whole-life RNAi) ...
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bioRxiv - Cell Biology 2023Quote: ... and M-MLV reverse transcriptase (Promega). Quantitative PCR was performed with iTaq Universal SYBR green supermix (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... and M-MLV reverse transcriptase (Promega). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2019Quote: ... of the entire or partial coding sequences cloned in pENTR vector or pGEM T-easy (Promega) and digested with respective endonuclease enzymes ...
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bioRxiv - Developmental Biology 2022Quote: ... the ARF3 coding region was amplified by RT-PCR and cloned into pGEM-T-easy (Promega). The resulting plasmid was digested with SpeI and transcribed with T7 RNA polymerase to generate the antisense probe ...