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Citations for Promega :
1 - 50 of 1814 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Then a model mRNA, coding for nanoluciferase (England et al., 2016) (Promega) was added to the aliquots to the final concentration of 30 nM ...
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bioRxiv - Genetics 2019Quote: ... transcribed mRNA into cDNA using M-MLV Reverse Transcriptase (Promega, Germany), and checked the effect on the psmc3 splice sites by RT-PCR using the amplification conditions as described before (Figure S14B).(Müller et al. ...
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... and mRNA translation started by adding 50 ng FLuc mRNA (Promega), followed by incubation at 30 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... PolyA+ mRNA was isolated using the PolyATtract mRNA Isolation System (Promega, Z5210). 20 µg of mRNA was used as input for miCLIP following the previously reported protocol 33 and an m6A antibody from Abcam (ab151230) ...
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bioRxiv - Neuroscience 2020Quote: ... with luciferase mRNA (Promega). Manufacturer’s instructions were followed with a few modifications ...
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bioRxiv - Molecular Biology 2024Quote: Rluc mRNA and Fluc mRNA were incubated with rabbit reticulocyte lysate (RRL, Promega) to generate the mRNA-ribosome complex ...
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bioRxiv - Cell Biology 2023Quote: ... 10 pg luciferase mRNA (Promega) was spiked into each fraction as a recovery control ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Microbiology 2019Quote: ... Translation of Firefly luciferase mRNA (Promega) was quantified using a Victor 2 microplate reader (PerkinElmer).
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Plant Biology 2022Quote: ... Isolation of mRNA was performed with the polyATract mRNA Isolation System IV (Promega Corporation, Madison, WI, USA) and was further processed after quality assessment and enrichment for pair-end cDNA library construction using the TruSeq RNA Library Preparation v2 Kit (Illumina ...
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bioRxiv - Genomics 2020Quote: Total mRNA was extracted from non-cross-linked embryos using the Maxwell Total mRNA purification kit (Promega, #AS1225) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... mRNAs were translated in wheatgerm extract (Promega) supplemented with 20 mM amino acids (lacking methionine ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA was isolated from ∼1 mg total RNA using oligo(dT) and streptavidin paramagnetic beads (PolyATtract mRNA Isolation Systems, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Capped mRNA was synthesized with Sp6 (Promega, WI) or T7 RNA polymerase (Roche ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 25 ng E.coli pre-ß-lactamase mRNA (Promega) were incubated with 2.5 µL rabbit reticulocyte lysate (Green Hectares) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was extracted using a PolyATract kit (Promega) and was reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... the mCherry coding sequence was PCR amplified from the plasmid pGFP-bait-MCS-NTR-mCherry using GoTaq® DNA Polymerase (Promega) with primers listed in Supplementary Table S3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Northern blot was performed using poly(A)+ mRNA isolated from 100 µg of total CaSki RNA with PolyATtract mRNA Isolation Systems (Promega, #Z5310) or 10 µg of total CaSki RNA as described previously 8 ...
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bioRxiv - Microbiology 2021Quote: ... and the extracted mRNA was used as a template for reverse transcription using M-MLV reverse transcriptase (Promega, Madison, WI, USA). Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... Spike-in RNA (polyadenylated kanamycin mRNA #C1381, Promega, USA) was added to the Cell Lysis Buffer with a final concentration 107 molecules/ml ...
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bioRxiv - Immunology 2024Quote: ... IVT mRNA (3 pmol) and 10U RNase inhibitor (Promega) were then added to the cell suspension ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... a known quantity of luciferase control mRNA (Promega, Madison, WI) was used as the internal standard to correct for RNA loss during extraction and purification procedure ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was treated with RQ1 RNase-Free DNase (M6101, Promega) and then used for cDNA synthesis with Superscript III Reverse Transcriptase (18080-044 ...
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bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of total mRNA were then reverse transcribed (Promega). Real time PCR was performed with SYBR green as the DNA binding dye (Roche Applied Science ...
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bioRxiv - Molecular Biology 2021Quote: ... CspA was allowed to bind cspA mRNA in 40 µL of Buffer N by incubating 10 pmoles of renatured mRNA with the indicated amounts of protein CspA in the presence of 0.4 U/µL of RNasin (Promega). After 15 min at 15°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... Capped mRNAs were generated using the Ribo m7G Cap Analog (Promega). The capped mRNAs were digested with DNase I and purified with the RNeasy kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... the mRNA was translated in a Flexi rabbit reticulocyte lysate (Promega) containing 35S methionine/cysteine (Perkin Elmer ...
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bioRxiv - Biochemistry 2021Quote: ... The acquired mRNA was then translated in rabbit reticulocyte lysates (Promega) containing 35S- labelled Met and Cys ...
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bioRxiv - Cell Biology 2021Quote: ... PolyA mRNA was detected using biotinylated oligo-dT probes (Promega #Z5261). Probe was diluted in ULTRAhyb-Oligo Hybridization Buffer (0.5 µL of 5 pmol/uL per mL of buffer) ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA transcripts were then translated in rabbit reticulocyte lysate (RRL) (Promega) in the presence of L-35S-methionine (PerkinElmer ...
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bioRxiv - Plant Biology 2023Quote: ... using the PolyATtract mRNA Isolation System III (Cat. No. Z5310; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was isolated using ReliaPrepTM RNA Cell Miniprep System (Promega, USA), and cDNA synthesis was performed using Superscript® First-Strand Synthesis System for RT-PCR (Thermo Fischer Scientific ...
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bioRxiv - Plant Biology 2020Quote: cysteine-substituted cpTatC mRNAs were transcribed in vitro with SP6 polymerase (Promega) and were translated in vitro in the presence of 3H-leucine (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: mRNAs were in vitro translated with Flexi Rabbit Reticulocyte Lysate System (Promega), as previously described (23 ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA was extracted using Reliaprep Minipreps for cell cultures (Promega, UK, #Z6012) and RNA Tissue Miniprep System (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA was then reverse-transcribed using the GoScript reverse transcriptase enzyme (Promega) and random primers ...
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bioRxiv - Microbiology 2022Quote: ... and linear DNA templates were transcribed into mRNA using T7 polymerase (Promega).
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was reverse-transcribed to cDNA by using oligo-dT primer (Promega) and M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by polyA enrichment using the PolyATtract® mRNA Isolation Systems (Promega). 9 μg of polyA RNA (1 μg/μl ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was yielded from purified mRNA using ImProm II Reverse Transcription kit (Promega). Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline) ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was amplified using GoTaq Green master mix (Promega, Leiden, The Netherlands; # M7112) and specific primers for the mRNA of interest (IDT ...