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Citations for Promega :
151 - 200 of 1206 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The A-tailed PCR products were then ligated to pGEM®-T Easy Vector using pGEM®-T Easy Vector Systems (Cat. A1360, Promega). Positive colonies were selected for Sanger sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... or the region between abaR and the abaI (for the abaI fusion) were amplified by PCR and ligated in pGEM®-T Easy using the pGEM®-T Easy Vector System (Promega). The resulting plasmids and the promoterless luxCDABE operon were digested with KpnI and BamHI and ligated in order to introduce the lux operon downstream of the predicted promoter of abaM or abaI ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed with TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse IgG (diluted 1:1,000 in TBS-T) (Promega, Madison, WI, USA) for 1 h at 37°C ...
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bioRxiv - Genetics 2021Quote: ... using the pGEM-T Easy Vector Systems protocol (Promega) and an LB-ampicillin selective medium ...
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bioRxiv - Molecular Biology 2019Quote: ... Products were cloned into pGEM-T Easy vector (Promega). Bacterial colonies obtained after transformation were screened by colony PCR for the presence of inserts of appropriate size ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy vector (Promega, Madison, WI, USA) were amplified by PrimeSTAR® MAX DNA Polymerase (TaKaRa Bio ...
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bioRxiv - Microbiology 2019Quote: ... which was then ligated into pGEM-T vector (Promega), generating pGEM-T-Δslo-2kb ...
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bioRxiv - Genetics 2019Quote: ... purified and cloned into pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Biochemistry 2020Quote: ... The pGEM-T Vector kit was purchased from Promega. Acrylamide/bisacrylamide (29:1 ...
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bioRxiv - Microbiology 2019Quote: ... These amplicons were cloned into pGEM-T Easy (Promega), and transformed into E ...
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bioRxiv - Genetics 2020Quote: ... A DNA fragment containing 200 bp of upstream HO flanking region and 200 bp of the HO gene was synthesized by Life Technologies and cloned into pGEM®-T Easy (Promega Corp.) as a NotI fragment (pCB4386) ...
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bioRxiv - Physiology 2019Quote: ... cloned into pGEM-T vector (Promega, Madison, WI, USA) and nucleotide sequence was confirmed by Sanger sequencing (Center for Applied Genomics ...
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bioRxiv - Genetics 2021Quote: ... and ligated into pGEM®-T Easy vector (Promega) using 1:3 molar vector:insert ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Amplicons were cloned into pGEM-T Easy vector (Promega) and sequenced.
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the pGEM-T Easy Vector (Promega) using DNA Ligation Kit
(Takara) ... -
bioRxiv - Microbiology 2020Quote: ... using the pGEM®-T easy Vector System (Promega). Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... sub-cloned into the pGEM T-Easy Vector (Promega), amplified with primers attB1 5’-TTACTCCATGTGTCAATACCAAAA-3’ and attB2 5’-GTCCATTTTAGTTCTCGAGTCGG-3 and introduced into the pDONR207 Gateway donor vector (Invitrogen) ...
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bioRxiv - Pathology 2022Quote: ... and cloned into a pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Genomics 2022Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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Cloning and In silico characterization of Heat shock factor (Hsf2) from Wheat (Triticum aestivum L.)bioRxiv - Molecular Biology 2020Quote: ... and cloned into pGEM-T simple vector (Promega, USA) in Escherichia coli strain M13 for sequencing.
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bioRxiv - Microbiology 2020Quote: ... cloned into pGEM-T-Easy (Promega - Madison, Wisconsin, USA) according to the manufacturer’s protocol and transformed into E ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Developmental Biology 2019Quote: Probe inserts were ligated into pGEM T-easy (Promega) according to kit instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Amplicons were ligated into pGEM T-easy vector (Promega) and Sanger sequenced.
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bioRxiv - Neuroscience 2021Quote: ... and cloned into the pGEM-T Easy vector (Promega) following manufacturer instructions and verified by sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... subcloned into the pGEM-T Easy Vector System (Promega) and sequenced on an ABI 3730 sequencer.
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bioRxiv - Plant Biology 2021Quote: ... pHSVd-monomer was based on pGEM-T vector (Promega) via insertion of HSVd cDNA cloned from HSVd-RZ plasmid via HSVd-f and HSVd-r primers (Supplemental Table 3) ...
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bioRxiv - Developmental Biology 2021Quote: ... then cloned into the pGEM-T easy vector (Promega). bmp2b and bmp4 were kindly provided by Matthias Hammerschmidt (Hild et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and cloned into pGEM®-T Easy Vector (Promega) using standard cloning procedures.
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bioRxiv - Genomics 2021Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... and cloned into the pGEM-T Easy vector (Promega). After sequence verification ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned into vector pGEM-T (Promega). CRISPR A and B start naturally with a 6 nt cassette consisting of adenines and guanines that we used for synchronisation of transcription (see below) ...
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bioRxiv - Microbiology 2023Quote: ... unlabeled PCR was cloned into pGEM-T Easy (Promega) and positive clones were checked by amplification with the vector-based primers M13f and M13R.
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bioRxiv - Evolutionary Biology 2023Quote: ... After cloning into pGEM-T vector (Promega, Madison, WI), sequences were analyzed by a commercial Sanger sequence service ...
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bioRxiv - Physiology 2023Quote: ... The PCR products were subcloned to T-vector (Promega) and sequenced ...
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bioRxiv - Genomics 2023Quote: ... TA-cloned into the pGEM-T Easy vector (Promega), and Sanger-sequenced with the M13R primer (HyLabs ...
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bioRxiv - Microbiology 2023Quote: ... before cloning into pGEM-T easy vector (Promega, USA). Plasmids prepared from selected clones were sent for sequencing using T7/SP6 primer (Macrogen ...
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bioRxiv - Zoology 2023Quote: ... then ligated to pGEM-T easy vector (Promega, USA) prior to transforming Escherichia coli-DH5α (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... pEGFP-N3 was from Clontech and pGEM-T Easy was from Promega (cat# A137A). New constructs were generated as described in Table S6 using routine molecular cloning techniques and enzymes from New England Biolabs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into the pGEM-T easy vector (Promega). After determining the nucleotide sequences using a 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... and ligated into the pGEM®-T vector (Promega). Linearized plasmids were then used as templates for in vitro transcription using either SP6 or T7 (Roche ...
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bioRxiv - Genomics 2023Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Molecular Biology 2023Quote: ... a pGEM-T Easy vector (Promega, Madison, WI, USA) with the blasticidin cassette flanked by a fragment of the 5’ UTR and 3’ UTR of the SUMO gene ...
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bioRxiv - Developmental Biology 2024Quote: ... miniata cDNA and cloned into pGEM-T Easy (Promega). Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... and ligated into the pGEM®-T vector (Promega) yielding pGEMT-KnpI-HP1x (where x is α ...
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bioRxiv - Immunology 2024Quote: ... Amplicons were cloned into pGEM-T-Easy (Promega, A1360) after adding an A-overhang with Taq DNA polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... and then the total cholesterol was quantified using the Cholesterol/Cholesterol Ester-Glo Assay Kit by Promega (J3190), according to the manufacturers instructions.