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Citations for Promega :
101 - 150 of 1206 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Then 5 μL of MTase reagent B (Promega) were added to all of the wells ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti b-galactosidase (1:500; Promega # Z3781), chicken anti b-galactosidase (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 5’ GA CTC GAG GTT GCA CCT AGT GGA T 3’ The PCR product was first cloned into pGEM-T easy vector (Promega), verified by sequencing and subsequently cloned into pGEX5.1 vector using the EcoRI and XhoI sites ...
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bioRxiv - Genetics 2020Quote: Via T/A ligation the fragment was inserted in the pGEM-T vector following the manufactures recommendations (Promega, Fitchburg, USA). Competent Escherichia coli cells (E ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40- pGEM-T Easy and MERS-CoV UpE-pGEM-T Easy were first linearized by Sal I enzyme (Promega) creating a 5’overhang ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... cloned into pGEM®-T easy vector (Promega). Five clones for each variety were sequenced and aligned with Fragaria x ananassa cv ...
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bioRxiv - Genetics 2021Quote: ... cloned into the pGEM-T Easy vector (Promega), and finally sequenced (SEQme ...
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bioRxiv - Physiology 2019Quote: ... or PGEM-T Easy Vector (Promega, Wisconsin, USA) using primers shown in Table 1 and cDNA from medaka brain and pituitary total RNA ...
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bioRxiv - Microbiology 2019Quote: ... Constructs were cloned into pGEM-T easy (Promega) or pJET1.2/blunt (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: Escherichia coli DH5α and pGEM-T easy (Promega) and pJET1.2/blunt (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... which was ligated into pGEM-T easy (Promega) and sequenced to ensure proper splicing ...
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bioRxiv - Plant Biology 2021Quote: ... A pGEM-T Easy vector (Promega, Madison, USA) cloned with a 450-bp fragment from SCYLV ORF3 previously amplified by RT-PCR was used to construct a serial dilution curve with six points and tenfold dilutions between points ...
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bioRxiv - Plant Biology 2021Quote: ... USA),cloned in pGEM-T easy vector (Promega, USA), and at least five individual clones were sequenced for each target.
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bioRxiv - Plant Biology 2020Quote: ... The amplificate was ligated into pGEM-T (Promega), from which the gene was excised with XhoI/EcoRI and ligated into pBAD/HisA (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
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bioRxiv - Biochemistry 2022Quote: ... The product was cloned into pGEM-T (Promega) and the resulting rbs_csp3 fragment sub-cloned into pDR111 ...
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bioRxiv - Synthetic Biology 2022Quote: ... or pGEM®-T Easy (Promega, Madison, WI) followed by sequencing of plasmids isolated from 10-15 colonies ...
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bioRxiv - Neuroscience 2021Quote: ... or pGEM®-T Easy Vector System (Promega). Nucleotide sequences of primers were summarized in Table 1 (No ...
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bioRxiv - Microbiology 2021Quote: ... ligated into the pGEM T Easy vector (Promega) and transformed in DH10B chemo-competent cells ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2019Quote: ... cloned into the pGEM T-Easy vector (Promega; according to the manufacturer’s instruction ...
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bioRxiv - Epidemiology 2019Quote: ... and cloned into pGEM-T vector (Promega, USA). In total six 16S rDNA and two rpoB clones were sequenced and analysed using the BLASTN algorithm and finally aligned using Spiroplasma glossinidia data from NCBI as reference.
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bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... and cloned in pGEM-T easy vector (Promega); Four to six positive clones were sequenced (MWG ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cloned into pGEM-T Easy vectors (Promega, USA) and then transformed into competent Escherichia coli cells for amplification ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Developmental Biology 2019Quote: ... and inserting it into pGEM-T Easy (Promega) by TA cloning ...
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bioRxiv - Cell Biology 2021Quote: ... Self-ligated empty pGEM-T vector (Promega, USA) was used to ensure equal amounts of transfected DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cloned into pGEM®-T Easy Vector (Promega), and then into the pSec-Tag2/hygroA vector (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and TA cloned into pGEM-T Easy (Promega) then transformed into DH5α competent E ...
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bioRxiv - Microbiology 2020Quote: ... Splice2 was cloned into pGem-T Easy (Promega) and integrity of the DNA confirmed by sequencing ...
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bioRxiv - Microbiology 2022Quote: ... cloned into the pGEM-T easy plasmid (Promega), and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the promoterless lacZ gene ...
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bioRxiv - Microbiology 2023Quote: ... The pGEM-T Easy Vector (Promega, Madison, USA) and pMG36e vector (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T Easy empty vector (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon cloned into pGEM T-EASY (Promega). An 804 bp portion of the hsdM1 coding sequence was deleted by inverse PCR with the inclusion of a unique BamHI site in each of the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pGEM-T Easy Vector (Promega). Positive clones were identified by the blue/white screening method and sequenced on ABI PRISM 3100 automated sequencer at Eurofins Genomics Europe Sequencing GmbH.
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bioRxiv - Molecular Biology 2023Quote: The pGEM®-T Easy Vector System (Promega) was used according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were cloned in pGEM-T (Promega), and the inserts were sequenced by Sanger sequencing (University of California Berkeley DNA Sequencing Facility) ...
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bioRxiv - Microbiology 2024Quote: ... ligated into the linearized vector pGEM-T (Promega) and transformed via electroporation into E ...
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bioRxiv - Bioengineering 2020Quote: ... Resuspended oligos were combined with an equal volume of the HaloTag ligand succinimidyl ester (O4) (Promega P6751) resuspended in N,N-dimethylformamide (Sigma-Aldrich D4551 ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA fragments generated by PCR were cloned into the pGEM-T Easy plasmid vector using the pGEM®-T Easy Vector System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: The prepared PCR fragments were inserted into the pGEM-T vector via T/A cloning following the manufacturer’s recommendations (Promega, Fitchburg, USA). Competent Escherichia coli were transfected with the ligation mix and plated on agar plates as described previously [13] ...
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: The N protein of SARS-CoV-2 B.1 D614G variant (NYI67-20 strain) was cloned into Nano-Luc vector pNLF1-N [CMV/Hygro] (N terminus) (Cat. N1351, Promega) as a fusion protein ...
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bioRxiv - Genomics 2020Quote: ... and unmethylated lambda DNA (Promega, Cat. N: D1521) was added to the samples (∼ 0.01%) ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Competent Cells, Promega, cat. n° L2005) and recombinant clones were checked by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...