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Citations for Promega :
51 - 100 of 4463 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... or at 25°C with Glu-C (Promega; 1:20 protease:protein w/w ratio) overnight ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 25 °C and digested with trypsin (Promega, 37 °C, overnight) in TEAB buffer (50 mM ...
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bioRxiv - Biochemistry 2019Quote: ... Trypsin/Lys-C (V5072 Promega) was directly spiked into the protein lysate at a ratio of Trypsin/Protein = 1:25 ...
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bioRxiv - Genetics 2020Quote: ... and Lys-C/trypsin (Promega, Madison ...
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bioRxiv - Molecular Biology 2023Quote: ... and pNLF1-C (N1361, Promega) to obtain N-terminal and C- terminal NanoLuc-tagged protein fusions ...
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bioRxiv - Biochemistry 2022Quote: ... trypsin/Lys-C mixture (Promega), ammonium bicarbonate (ABC ...
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bioRxiv - Cancer Biology 2022Quote: ... Trypsin/Lys-C solution (Promega) was added and incubated at 37°C for 12 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Genomics 2021Quote: ... 8 μg of RNA were treated for 1 h at 37°C with 12 units of RQ1 DNase (Promega®) followed by phenol-chloroform extraction and ethanol precipitation of RNA which was subsequently dissolved in water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or RepNDK-RNA was incubated at 37 °C for 12 h in a translation system and FluoroTect GreenLys tRNA (Promega), with neither CTP nor CDP to preclude RNA replication ...
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Cell Biology 2022Quote: ... ATP levels were assayed using the Mitochondrial ToxGlo™ Assay kit (Promega) as per manufacturer’s instructions on a FLUOstar OPTIMA (BMG LABTECH ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Digestion was performed for ∼18 hours at 37 °C using sequencing grade Glu-C (Promega). Peptides were analysed by nanoHPLC-MS/MS over a 65 min acquisition with elution from a 50 cm C18 PepMap column onto an Orbitrap Fusion Tribrid mass spectrometer via an Easy-Nano ionisation source ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin or Lys-C alone (Promega). Digested peptides were separated by liquid chromatography and analyzed on an Orbitrap Elite mass spectrometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... 2) chymotrypsin and lysyl endopeptidase (lys-C) (Fujifilm Wako) 3) lys-C and trypsin (Promega) and 4 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C.
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bioRxiv - Cancer Biology 2020Quote: ... predigested with Lys-C (Wako) (1:75, 4h at 37°C) and trypsin digested overnight (Trypsin Gold, Mass Spectrometry Grade, Promega; 1:50 at 37°C). Peptide samples were desalted using C18 Sep-Pak cartridges (3cc ...
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bioRxiv - Cancer Biology 2020Quote: ... predigested with Lys-C (Wako) (1:75, 4h at 37°C) and trypsin digested overnight (Trypsin Gold, Mass Spectrometry Grade, Promega; 1:50 at 37°C). Peptide samples were desalted using C18 Sep-Pak cartridges (3cc ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...
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bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
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bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
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bioRxiv - Neuroscience 2021Quote: ... S7A–C using FuGene 6 (Promega). Two days after transfection ...
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bioRxiv - Systems Biology 2022Quote: ... and Lys-C (VA1170) from Promega. The beads used here include Sero-Mag streptavidin magnetic beads (Cytivia) ...
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bioRxiv - Biochemistry 2019Quote: ... and Arg-C were from Promega. Tris ...
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bioRxiv - Systems Biology 2020Quote: ... Trypsin/Lys-C Mix enzyme (Promega) was then added to the beads for a 16-hour on-beads digestion at 37 °C in a ThermoMixer shaking at 1200 rpm ...
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bioRxiv - Microbiology 2020Quote: ... 0.25 μg Lys-C protease (Promega) was added and incubated for 4 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and Lys-C (Promega, Cat# PI90307). In a 50 µL reaction solution ...
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bioRxiv - Molecular Biology 2022Quote: ... and pNLF1-C [CMV Hygro] (Promega) vectors using restriction cloning or the In-Fusion HD Cloning Plus kit (Takara Bio ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequential digestion with Lys-C (Promega) and trypsin (proteomic grade from porcine pancreas ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Genomics 2019Quote: To generate recombinant p53 we in vitro transcribed/translated human p53 with a c-terminal HA tag using a rabbit reticulocyte system (Promega). To generate fragmented genomic DNA we tagmented 50ng of human genomic DNA from MCF7 cells using the MuSeq kit (Thermo ...
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bioRxiv - Immunology 2022Quote: ... and the mitochondrial ToxGlo™ (Promega) assay were used accordingly to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... Resins were transferred to a new tube and proteins were digested with 0.1 µg Lys-C for 2h at 37°C and 0.5 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were digested overnight at 37 °C using MS-grade trypsin/Lys-C Mix (Promega, Madison, WI) (enzyme/substrate of 1:50 w/w ...
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bioRxiv - Plant Biology 2023Quote: ... In-gel digestion of proteins was performed at 37 °C overnight using 2.5 μg Trypsin/Lys-C (Promega). Samples were further digested with 2.5 μg Chymotrypsin (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 100 mM HEPES pH 7.5 and digested at 37°C overnight with Trypsin/Lys-C (Promega) at a protease-to-proteome ratio of 1:100 (wt/wt) ...
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bioRxiv - Biochemistry 2021Quote: ... then 750 ng Glu-C (Promega, V1651) were added and incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.5 µL 5ng/µL Gluc-C (Promega) was added and incubated for three hours at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing-grade trypsin/Lys-C mixture (Promega) was added at 1:50 protease to protein ratio before incubation at 37°C for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... Initial digestion with 15μg Lys-C (Promega) was performed by incubation 2h RT ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 37°C or trypsin (Promega) for 1 hr at 37°C ...
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bioRxiv - Physiology 2024Quote: ... with 1 µg Lys-C/Trypsin (Promega) and incubated at 37°C overnight.
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was collected and used for taking the luciferase reading using Dual Luciferase kit (Promega) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...