Labshake search
Citations for Promega :
401 - 450 of 1480 citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Nluc was amplified from the pNL1.1 plasmid (Promega, Madison, WI) with primers to ensure in-frame Nluc expression as well as containing 25 bp overlap to the insertion site to allow cloning by Gibson Assembly ...
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bioRxiv - Microbiology 2019Quote: ... together with pRL-CMV reference plasmid (0.03 µg/well; Promega). Cells were simultaneously co-transfected with 0.3 µg/well of the empty pCI-neo-3xFLAG expression vector or encoding viral proteins as specified ...
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bioRxiv - Microbiology 2019Quote: ... Amplified plasmid was then phosphorylated using T4 polynucleotide kinase (Promega) and self-ligated (T4 DNA ligase ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The sequence was subcloned into the pCMVTNT expression plasmid (Promega). Other plasmids used in this study express X ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with plasmids of interest using Fugene (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR products were cloned into pGEM®-T plasmid (Promega) and transformed into TOP10 chemically competent E ...
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bioRxiv - Microbiology 2020Quote: ... as carrier DNA and a luciferase-encoding plasmid (pGL4.26, Promega). qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293FT cells were transfected with the plasmid using FuGENE6 (Promega). After 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 ng pGL3-Basic luciferase reporter plasmid (Promega, Cat. #E1751) with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega ...
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bioRxiv - Genetics 2020Quote: ... and 100ng of Renilla luciferase control plasmid (Promega; Accession # AF025848) was mixed with 3μL of BioT transfection reagent (Bioland Scientific LLC ...
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bioRxiv - Cell Biology 2020Quote: Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (cat# E8491, Promega) was used as template DNA in a PCR for obtaining the minimal promoter Luc2P together with the NF-κB response elements (NF-κB-RE) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with psiCHECK-2 plasmids (Promega, Madison, USA) containing two luciferase genes ...
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bioRxiv - Biochemistry 2023Quote: ... After overnight attachment the dual reporter plasmids (pGL4.36, pGL4.74 (Promega)) ...
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bioRxiv - Microbiology 2023Quote: ... and isolated with PureYield Plasmid Miniprep System (Promega, Cat# A1222). Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and the phRL-TK Renilla luciferase internal control plasmid (Promega). Reporter activity was evaluated 48 hours after transfection using a dual-luciferase assay according to the manufacturer’s protocol (Promega) ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was cloned into pGEM-T plasmid (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of Renilla luciferase expression plasmid (pRL-TK) (Promega) was co-transfected for transfection efficiency normalization (500 ng of DNA total was added per transfection) ...
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bioRxiv - Genetics 2023Quote: ... and the PureYield® Plasmid Midiprep Protocol System (Promega, USA). Verification of successful cloning was done by sequencing (Eurofins ...
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bioRxiv - Plant Biology 2023Quote: ... coli using PureYield™ Plasmid Miniprep (Promega, Madison, WI, USA), verified using Sanger sequencing (Laboratory of Biotechnology & Bioanalysis ...
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bioRxiv - Neuroscience 2023Quote: PCR reactions were ligated to pGEM T-Easy plasmid (Promega). Closed inserts were Sanger sequenced by the UChicago Comprehensive Cancer Center DNA Sequencing Facility ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of the IFN-β reporter plasmid (Promega, USA) and 4 ng of Renilla luciferase plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega) on an Amaxa Nucleofector 2b (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were done using FuGENE HD (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega) using lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and pooled human male DNA (G3041, Promega and 4312660, Life Technologies) were used to generate calibration curves and serve as reaction controls ...
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bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Human normal genomic DNA was obtained from Promega (Madison, WI, USA) and the values are averages of three replicates ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Cancer Biology 2020Quote: ... in a background of 130ng of normal human genomic DNA (Promega) was used as a positive control ...
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bioRxiv - Cell Biology 2019Quote: ... and human samples were performed using GoTaq green master mix (Promega) and the following amplification conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... 830bp or 800bp human STIM1 promoter into pGL3 basic vector (Promega) between XhoI and HindIII ...
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bioRxiv - Systems Biology 2020Quote: ... albicans as well as of a commercially available human protein digest (Promega), which was exclusively used in this experiment ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Biochemistry 2023Quote: MS-Compatible Human Protein Extract Digest (K562) was purchased from Promega (V6951), MassPREP E ...
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bioRxiv - Cell Biology 2023Quote: ... human BLTP2/KIAA0100 ORF was amplified from pFN21A-Halo-KIAA0100 (FHC00016, Promega). An unstructured linker (sequence ...
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bioRxiv - Plant Biology 2020Quote: ... the PCR products were cloned into pGEM-T Easy plasmid (Promega) for propagation and sequencing (GATC Biotech ...
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bioRxiv - Synthetic Biology 2021Quote: All Plasmids were purified using the Midiprep kit (QIAGEN or Promega)
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bioRxiv - Cancer Biology 2021Quote: Plasmids were transiently transfected into 293T17 cells using FuGENE 6 (Promega). For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites ...
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bioRxiv - Cancer Biology 2021Quote: ... The pSLIK-Hygro plasmid was transiently transfected by Fugene HD (Promega) in HEK293T cells together with the VSV-G ...
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bioRxiv - Genetics 2022Quote: ... and directionally cloned into the pGL3-Promoter plasmid (pGL3-P) (Promega) upstream of the SV40 promoter using restriction enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were cloned into a pGEM-T Easy plasmid (Promega, A1360). Ligation reactions were transformed into chemically competent cells for blue-white colony screening.
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bioRxiv - Molecular Biology 2020Quote: ... 100ng of the renilla luciferase expressing plasmid (pRL-SV40 Promega, E2231) was included as a transfection control ...
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bioRxiv - Biochemistry 2019Quote: ... Transfection of plasmids into cells were performed using FuGENE 6 (Promega) according to the manufacturer’s protocol and the cells were harvested 24 hours after transfection ...
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bioRxiv - Immunology 2019Quote: ... The NF-κB-Luc luciferase reporter plasmid was purchased from Promega.
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bioRxiv - Microbiology 2019Quote: ... 25 ng of a constitutively expressing Renilla luciferase plasmid (pRLTK, Promega) and the indicated viral protein expression plasmids – HEK293T cells ...
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bioRxiv - Genetics 2019Quote: ... plus pQCXIN-BirA plasmids using Fugene HD (Promega, Madison, WI, USA). Virus-containing medium was collected ...
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bioRxiv - Genetics 2020Quote: ... Each well was co-transfected with the pRL-TK plasmid (Promega) expressing the renilla luciferase as control ...