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Citations for Promega :
201 - 250 of 1480 citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... We added 4μl pooled commercial human gDNA (Promega) to all samples to ensure total gDNA > 1μg/35μl ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... Human GSK-3β was purchased from Promega (V1991).
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bioRxiv - Genomics 2020Quote: ... and human gDNA (Promega, San Luis Obispo, CA) was added to reach a total input of 3 μg/130uL for fragmentation and library prep ...
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bioRxiv - Biochemistry 2021Quote: ... and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Hela cells were co-transfected with CRISPR-Cas9 plasmids and a plasmid conferring puromycin resistance using FuGENE (Promega, E2693). 24 h after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Molecular Biology 2019Quote: ... The recombinant plasmids were isolated from the positive clones using the Pure Yield Plasmid Miniprep System (A1222, Promega, USA), and some potential positive plasmids containing the cDNA insert were digested with EcoRI and HindIII to confirm the presence of the IFNε cDNA insert.
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bioRxiv - Immunology 2022Quote: ... 300 ng of this plasmid together with 30 ng of a renilla luciferase control plasmid (pGL4.74[hRluc/TK], Promega) were transfected in keratinocytes (24 wells ...
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bioRxiv - Molecular Biology 2022Quote: ... the Renilla luciferase (RLuc) splicing reporter plasmids were co-transfected with a Firefly luciferase plasmid (pGL3 control-FLuc, Promega) as transfection control ...
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bioRxiv - Cell Biology 2019Quote: ... Either HCT116 cells or DLD-1 cells were transfected with guide plasmids and donor plasmid using ViaFectTM (#E4981, Promega) on 3.5cm dishes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T cells were transfected with an equal ratio of indicated GPCR plasmid and pGloSensor™-22F cAMP plasmid (Promega). 18 hours post-transfection ...
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bioRxiv - Genomics 2022Quote: ... expression plasmids were co-transfected with GAG/POL and VSVG plasmids into HEK 293T cells using FuGENE HD (Promega). Media was exchanged after 6-10 h ...
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bioRxiv - Molecular Biology 2022Quote: Cells were co-transfected with 1.0 μg/35-mm dish of the effector plasmids and 1.0 μg/dish of the pGL3-CHOP luciferase reporter plasmids or pGL3-Basic (Promega) with a simian virus 40 (SV40 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transfected with the following plasmids and amounts per well: 50 ng GloSensor −20F cAMP plasmid (E1171, Promega), 125 ng Gαi or Gαi QL in pcDNA3.1+ ...
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bioRxiv - Cell Biology 2023Quote: ... The following day cells were co-transfected with 0.95 μg of a Firefly luciferase reporter plasmid and 50 ng phRL-SV40 plasmid (Renilla luciferase, Promega) as an internal control for transfection efficiency using FuGene6 (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 105 HEK293T cells were seeded into 24-well plates and co-transfected with pGL4.10-based plasmids and pRL-SV40 Renilla luciferase plasmid (Promega). After 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then transfected with plasmid DNA (4µg per plasmid per flask) using FuGENE HD (1:3 ratio; Promega) and cultured at 28°C.
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... The recombinant plasmids were prepared from some positive clones using the PureYield Plasmid Miniprep System (Cat #A1222, Promega, Madison, USA). The sequencing of the cloned insert was carried out according to Sanger et al ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were then co-transfected with the corresponding pHR plasmid and lentiviral packaging plasmids (pMD and CMV) using Fugene HD (Promega). Cells were incubated for ~ 48 hours and virus was collected and filtered through a 0.45 mm filter ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells were transfected with mCherry or Cre-T2A-mCherry containing FUGW vector plasmids and helper plasmids PAX2 and VSVG using Fugene6 (Promega). Supernatant was collected 48 hours after transfection ...
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bioRxiv - Bioengineering 2020Quote: VSV-G-pseudotyped lentiviral vectors were produced by co-transfecting HEK293T cells with lentiviral backbone plasmids and packaging plasmids (pHDM-Hgpm2, pHDM-tat1b, pRC/CMV-rev1b, pHDM-G) using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The WT or mutant plasmids were then transfected into WT and SIRT1 KO mESCs together with the control pRL-TK plasmid (Renilla Luciferase, Promega). Cells were cultured for 48 h and the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with plasmids DNA (250 ng per plasmid per well) using FuGENE HD (Promega, 1:8 ratio), and cultured at 28°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Immunology 2022Quote: ... pHR-based lentiviral plasmids containing these transgenes were transfected into HEK293T cells together with pMD2G and psPAX2 helper plasmids using Fugene (Promega). After 2 days ...
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bioRxiv - Synthetic Biology 2020Quote: ... were grown in 5 mL LB medium overnight and plasmid DNA was isolated using the PureYield Plasmid Miniprep System (Promega). The plasmids were further tested by a restriction enzyme digestion analysis ...
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bioRxiv - Biochemistry 2019Quote: ... At that point they were co-transfected with desired pHR plasmid and lentiviral packaging plasmids (pMD and CMV) using Fugene HD (Promega). Virus was collected after approximately 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118). Construction of the GLP-1R-SmBiT plasmid was described previously (55 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118); this configuration was used due to previous success with another class B GPCR (Shintani et al. ...
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bioRxiv - Cell Biology 2021Quote: The luciferase reporter plasmid was generated by cloning the mouse HDAC7 promoter into the NheI site of pGL4 Luciferase reporter plasmid (Promega). The mouse HDAC7 promoter was amplified using Platinum SuperFi II DNA Polymerase (12361010 ...
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bioRxiv - Molecular Biology 2021Quote: ... the transfer plasmids of interest were co-transfected with GAG/POL and VSVG plasmids into 293FT cells using FuGENE HD (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Retrovirus was generated by transfecting SETBP1 pMSCV-IRES-mCherry or CSF3R pMSCV-IRES-GFP or control vector plasmids and the EcoPac plasmid (provided by Dr. Rick Van Etten) into the 293T17 cells using FuGENE 6 (Promega). Viral supernatants were harvested 48 and 72 hours later ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... The bacterial pellets were suspended in sterile water and plasmids were extracted using a PureYield™ Plasmid Miniprep System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... the resulting plasmid and the IF-GFP-ATRX plasmid (a gift from Michael Dyer43, addgene plasmid #45444; exon 6 absent) were digested with XhoI (Promega) and SpeI (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with act5c-Gal4 plasmid (600 ng) and pUAST-based expression plasmid (1000 ng) using FugeneHD reagent (Promega). Transfected cells were cultured at 25°C and processed within 1 to 3 days.
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bioRxiv - Microbiology 2023Quote: ... a pPolI-Firefly plasmid encoding a negative-sense viral-like RNA expressing the Firefly luciferase and the pTK-Renilla plasmid (Promega) as an internal control ...
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bioRxiv - Immunology 2023Quote: ... Select colonies were then grown in 3 ml of LB with appropriate antibiotics and the plasmids were purified with PureYield Plasmid Miniprep System (Promega), performing restriction enzyme digests to verify that the plasmids had inserts.
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bioRxiv - Cancer Biology 2023Quote: ... we transfected 500,000 ANBL6 cells with 1 ug of reporter plasmid plus 250 ng of Rinella plasmids pRL-SV40 and pGL3 control (Promega) in biological triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...
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bioRxiv - Molecular Biology 2023Quote: ... a pPolI-Firefly plasmid encoding a negative-sense viral-like RNA expressing the Firefly luciferase and the pTK-Renilla plasmid (Promega) as an internal control ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miRNA plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... were seeded onto 12-well plates at 150.000 cells per well and transfected the next morning with 1μg of the BSEPprom-Luc or SHPprom-Luc plasmid and 100ng each of FXR and RXR expression plasmids using Fugene HD (Promega) at a ratio of 2.5:1 (reagent:DNA) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REX HeLa cells were transfected with 1.8 μg of pOGG4 plasmid and 200 ng of pcDNA5/FRT/TO plasmid encoding the relevant transgene cassette using FuGENE HD transfection reagent (Promega). Cells with a stably integrated transgene were selected with 150 μg/mL hygromycin for 4-5 days ...