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Citations for Promega :
501 - 550 of 6245 citations for Human H ACA Ribonucleoprotein Complex Subunit 1 GAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined after 72 h using the CellTiter-Glo® luminescent assay (Promega, catalog number G9242). Cell viabilities of DMSO-treated cells were set to 100% ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were treated with 3 µM PP242 for 2 h and lysed with Passive Lysis Buffer (Promega, E194A). Luminescence was detected with a Dual- Luciferase Reporter Assay System (Promega ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells incubated for 18 h and harvested for cell viability quantitation using Cell-Titer-Glo (Promega; 20 µL) on a Synergy Neo plate reader (Biotek).
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bioRxiv - Biochemistry 2021Quote: Cells were transfected for 48 h with 15 µg DNA per 15 cm dish using Fugene HD (Promega) following the manufacturer’s instructions using plasmids encoding full-length FLAG-tagged MeiP26 protein ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBST and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibody (Promega) was incubated with the membrane at a 1:5,000 dilution for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... Cell death after 5 h treatment was quantified by PI incorporation or using the Caspase Glo system (Promega) according to manufacturer instructions.
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bioRxiv - Genomics 2021Quote: ... Luciferase activity was measured 36 h after transfection using the dual-luciferase reporter system (Promega, Madison, WI, USA). Firefly luciferase activity was normalized to Renilla luciferase activity.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined after 72 h using the CellTiter-Glo® luminescent assay (Promega, catalog number G9242). Cell viability of DMSO-treated cells was set to 100% ...
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bioRxiv - Bioengineering 2023Quote: ... Fluc luminescence was measured 72 h after co-culture using the Bright-Glo Luciferase Assay System (Promega, E2610) and a Tecan Infinite M200 Pro plate reader ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative luciferase activity was measured 24 h post-transfection with the Dual-Glo Luciferase Assay System (Promega, USA).
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured 72 h after drug treatment using CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9241) following manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed at 72 h post-treatment by performing CellTiter-Glo Luminescent Cell Viability Assays (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega, Madison, WI).
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed on 1 µg of RNA using ProtoScript First Strand cDNA Synthesis Kit (Promega). Primers recognizing exons flanking the CRISPR-targeted exon (Supp ...
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bioRxiv - Microbiology 2019Quote: ... 1 mL of culture was used for genomic DNA isolation with the Wizard DNA Purification Kit (Promega). Approximately 2 µg of DNA was used to prepare a paired-end 250-bp sequencing library with the Nextera XT DNA library kit ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µg of total RNA was used to synthesize cDNA using M-MLV reverse transcriptase kit (Promega) using the supplier’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... CYP3A4 and CYP2C9 activities were measured by incubating cultures with luciferin-IPA or luciferin-H substrates (Promega, Madison, WI) for 1 or 3 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... the RNA/oligonucleotide mixture was diluted to 30 µl final with a reaction mix (1X RNase H reaction buffer, 65 µM DTT, 0.5U/µl RNasin (Promega), 50 U RNase H (New England Biolabs ...
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bioRxiv - Cancer Biology 2019Quote: ... and cultured for 72 h at 37°C before viability assay using the ATP-based CellTiter-Glo (CTG) 2.0 Assay (Promega). Luminescence was read with the LMAX II luminometer (Molecular Devices) ...
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bioRxiv - Bioengineering 2020Quote: ... The transduction efficiency was quantified at 16 h posttransduction by measuring firefly luciferase activity according to the manufacturer’s instructions (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were collected 24 h after transfection and luciferase activities were measured with Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 24 h later and subjected to luciferase assays using the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Plant Biology 2021Quote: The RNA was transcribed into cDNA using the M-MLV Reverse Transcriptase (RNase H- Point Mutant, Promega, Walldorf, Germany). qPCR was performed using a Realplex Mastercycler system (Eppendorf ...
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bioRxiv - Neuroscience 2021Quote: ... Dose response curve for H2O2 was performed 24 h after the H2O2 treatment by using CellTiter Blue (Promega, G8080). CellTiter blue reagent (diluted 1:4 with PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were carried out at 48 h after infection following the manufacturer’s instructions with the Dual-luciferase Reporter Assay System (E1910; Promega). Firefly luciferase activity was normalized by Renilla luciferase activity.
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bioRxiv - Cell Biology 2020Quote: ... The sample was first treated by 2.5 μg of LysC (Wako) for 3 h at 37 °C with shaking and then treated with 2.5 μg of Trypsin (Promega) for over-night at 37 °C with shaking ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were harvested 24 h post transfection and luciferase assay was performed as per the manufacturer’s protocol (Promega, E2610). Readings for luminescence were taken in luminometer (Berthold ...
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bioRxiv - Microbiology 2021Quote: ... Dual luciferase reporter assays were performed 48 h post transfection using the Dual Luciferase Reporter Assay System following the manufacturer’s protocol (Promega). The renilla (primary reporter ...
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bioRxiv - Molecular Biology 2021Quote: ... Digestion was performed for 8 h at 37°C using 70 ng modified porcine trypsin (Promega, Fitchburg, WI, USA). Tryptic peptides were extracted using 70% acetonitrile and dried using a SpeedVac vacuum concentrator.
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bioRxiv - Microbiology 2020Quote: ... Samples were digested initially with LysC (WAKO) at 37°C for 2.5 h and subsequently with 10 μg trypsin (modified sequencing grade, Promega) overnight ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized by reverse transcription using RevertedAid H minus reverse transcriptase (ThermoFischer Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3’ end of the MERS-CoV genome ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected at 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Assay (Promega) with a Luminoskan Ascent luminometer (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: Cells transfected with corresponding plasmids for 24 h were applied to cytotoxicity test using CytoTox 96® Reagent (Promega) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Microbiology 2023Quote: ... The virus infectivity was determined 48 h later by quantifying the luciferase activity (luciferase assay system, Promega, Madison, WI). Neutralization curves were normalized to virus only control ...
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bioRxiv - Immunology 2023Quote: ... All conditions used were tested for cytotoxicity by measuring lactate dehydrogenase in the conditioned media after 24 h incubation according to the manufacturer’s instructions (CytoTox96, Promega) (Figure S1).
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured 24 h after transfection with the Dual-Glo luciferase assay system (Promega) using a Luminometer (Berthold Technologies).