Labshake search
Citations for Promega :
51 - 100 of 6015 citations for Human Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... or the TnT T7 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the T7 promoter ...
-
bioRxiv - Developmental Biology 2019Quote: ... FluoroTect™ Green Lys in vitro Translation Labelling System (Promega) was used for protein labelling in place of 35 S-methionine in the original protocol ...
-
bioRxiv - Cell Biology 2021Quote: A TNT T7 Quick Coupled Transcription/Translation System (Promega, L1170) with Transcend tRNA (Promega ...
-
bioRxiv - Biochemistry 2022Quote: ... the translation mixture was supplemented with FluoroTect GreenLys reagent (Promega). Purified samples were washed with TBS (50mM Tris and 150mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... In vitro translation was performed in rabbit reticulocyte lysate (Promega) supplemented with 1 mCi/ml [35S]Met/Cys ...
-
bioRxiv - Cell Biology 2019Quote: ... in vitro translation was performed in nuclease-treated RRL (Promega) using 150 ng of luciferase mRNA in the presence of 15 μCi of [35S]-Met for 60 min at 30°C ...
-
bioRxiv - Biochemistry 2021Quote: ... In vitro translation assays with wheat germ extract (Promega®) were performed according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: Translation was performed in Flexi® Rabbit Reticulocyte Lysate (Promega) supplemented with amino acid mixture minus methionine and leucine ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
-
bioRxiv - Molecular Biology 2020Quote: ... We performed in vitro transcription/ translation using the TNT system (Promega) with pcDNA3 expression vectors for murine Flag-tagged Tet catalytic domains or full-length Halo-tagged human Tet proteins ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli employed a coupled T7/S30 in vitro translation system (Promega). A ~450 bp fragment containing the EMCV PRF signal (or mutant derivative ...
-
bioRxiv - Cell Biology 2022Quote: ... generated using the TNT T7 Quick Coupled Transcription/Translation System (Promega), was imported into isolated mitochondria for 20 min at RT and import was stopped by the addition of 0.5 μM valinomycin ...
-
bioRxiv - Molecular Biology 2020Quote: The coupled Transcription/Translation system (T7 Quick for PCR DNA, Promega) was used to express ATFS-1 from a PCR template ...
-
bioRxiv - Cell Biology 2020Quote: ... the T7 TnT Quick coupled in vitro transcription/translation system (Promega) was used according to the suppliers instructions.
-
bioRxiv - Biophysics 2020Quote: ... using the nuclease treated RRL in vitro translation system from Promega. The RRL was made more cap-dependent by addition of 75 mM KCl (58) ...
-
bioRxiv - Biochemistry 2023Quote: ... and mRNA translation started by adding 50 ng FLuc mRNA (Promega), followed by incubation at 30 °C for 2 hours ...
-
bioRxiv - Developmental Biology 2021Quote: ... pCDNA-Cnot3 Δ701-751 and pCDNA-Cnot3Δ651-751 were in vitro transcribed/translated using a TNT® Quick Coupled Transcription/Translation kit (Promega, USA) according to manufacturer’s instructions using 10μCi of 35S-Methionine to radiolabel the proteins ...
-
bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2019Quote: ... Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with DTT (10 mM ...
-
bioRxiv - Biochemistry 2019Quote: Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with 10 mM DTT (Melford ...
-
bioRxiv - Molecular Biology 2021Quote: In vitro translation in RNase-treated Rabbit Reticulocyte Lysate System (RRL, Promega) was carried out as recommended by the manufacturer with slight modifications ...
-
bioRxiv - Physiology 2022Quote: ... The TnT T7 Quick Coupled Transcription/Translation System (catalog no. L1170, Promega) was used with pcDNA3.1(+)-FLAG-mGCNA and pcDNA3.1(+)-FLAG-mSPRTN E113Q plasmids in a 40 μl reaction according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Coupled transcription/translation reactions were carried out per manufacturer’s instructions (Promega, L1170). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... TnT® T7/SP6 Quick Coupled Transcription/Translation System (L1170/L2080) (Promega).
-
bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
-
bioRxiv - Cell Biology 2019Quote: ... or EED that was in vitro-transcribed and translated according to the manufacturer’s procedures (TNT T7 Quick Coupled Transcription/Translation Kit; Promega, Leiden, the Netherlands) at 4°C overnight ...
-
bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... In vitro translation reactions were performed in nuclease-treated Rabbit Reticulocyte Lysate (Promega) as described by the manufacturer ...
-
bioRxiv - Cancer Biology 2019Quote: ... Translation read-out was performed using the dual-luciferase reporter assay system (Promega) as per the manufacturer’s protocol on a Tecan M200 multimode reader running Tecan Magellan software (Tecan) ...
-
bioRxiv - Plant Biology 2021Quote: ... The MYB -expression vectors were used in a coupled transcription/translation system (Promega) to produce MYB Halo tagged proteins ...
-
bioRxiv - Plant Biology 2021Quote: ... and the TnT® Quick Coupled Transcription/Translation System (for T7 promoter, Promega) according to the manufacturers protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... The MYB24-expression vector was used in a coupled transcription/translation system (Promega) to produce MYB24 protein with the HaloTag-fusion ...
-
bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
-
bioRxiv - Biochemistry 2023Quote: In vitro translation assays were performed using nuclease-treated rabbit reticulocyte lysate (Promega). Reactions were in 10 µl containing 2 µl RRL ...
-
bioRxiv - Biochemistry 2023Quote: ... In vitro protein synthesis by the coupled transcription and translation (TnT) system (Promega) was carried out in the presence of 35S methionine in either wheat germ or rabbit reticulocyte lysates ...
-
bioRxiv - Plant Biology 2023Quote: ... we used in vitro translation system based on Wheat Germ Extract (Promega Corporation), which has been used for SCR validation as reported previously31 ...
-
bioRxiv - Molecular Biology 2024Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). Capped and polyadenylated reporter mRNAs containing the first 135 nucleotides of NPR coding sequences (human ...
-
bioRxiv - Immunology 2020Quote: ... were verified by Sanger sequencing and used as DNA templates in the T7 TNT in vitro transcription/translation kit (Promega, Madison, WI; #L1170) using [35S]-methionine (PerkinElmer ...
-
bioRxiv - Genetics 2021Quote: The in vitro protein translations were performed using the TNT-assay (L4610) from Promega according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Myc-tagged MiD49 and MiD51 were produced via in vitro transcription/translation (IVT) (Promega). For in vitro ubiquitination assays ...
-
bioRxiv - Biophysics 2020Quote: ... In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega) supplemented with canine rough microsomes (tRNA probes ...
-
bioRxiv - Plant Biology 2021Quote: Reactions were performed using the TNT® T7 Quick Coupled Transcription/Translation System (Promega) with 1 μg of vector (NPH3 or variants in pGADT7 ...
-
bioRxiv - Biochemistry 2020Quote: ... except that the efficiency of translation of 100 ng/μl firefly luciferase mRNA (Promega) in RRL was measured (in relative luminescence units ...
-
bioRxiv - Cell Biology 2021Quote: Proteins were synthesized using TNT® SP6 Quick Coupled Transcription/Translation system (Promega L2080) using the standard reaction mix (rabbit reticulocyte lysate plus amino acids ...
-
bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
-
bioRxiv - Cell Biology 2022Quote: ... In vitro translation was done using the TNT T7 Reticolucyte Lysate System (Promega Corp.), in the presence of radioactive 35S-methionine ...
-
bioRxiv - Molecular Biology 2019Quote: ... The translated protein was analyzed using a Transcend Non-Radioactive Translation Detection System (Promega).
-
bioRxiv - Molecular Biology 2021Quote: In vitro translation reactions were performed using nuclease-treated rabbit reticulocyte lysate (RRL) (Promega) following the manufacturer’s protocol for non-radioactive luciferase reactions ...
-
bioRxiv - Cell Biology 2022Quote: ... Constructs for in vitro translation reactions were based on the SP64 vector (Promega, USA). Templates for transcription were generated by PCR ...