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Citations for Promega :
1 - 50 of 6015 citations for Human Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... and a vector to aid with translation initiation (pAdVAntage, Promega) into 293T cells using GeneJuice transfection reagent (Novagen ...
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bioRxiv - Cell Biology 2023Quote: ... and a vector to aid with translation initiation (pAdVAntage, Promega) into HEK-293T cells using GeneJuice transfection reagent (Novagen ...
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bioRxiv - Microbiology 2021Quote: ... Eukaryotic in vitro Nluc expression was performed with TnT-T7 Quick Coupled Transcription/Translation System (# L1170, Promega) with 100 ng of pT7CFE1-NLuc plasmid DNA ...
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bioRxiv - Microbiology 2023Quote: Translation reactions were performed using the TNT Quick Coupled Transcription/Translation kit (Promega). 10-50 ml reactions were assembled according to kit directions and labelled using EasyTag L-[35S]-Methionine (Perkin-Elmer) ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Genetics 2019Quote: In vitro transcription and translation were realized from 500ng of cDNA pGEM-T construct using the TnT T7 Quick Coupled Transcription/Translation kit (Promega) and Transcend Biotin-Lysine-tRNA following the manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... expressed using the TNT Quick Coupled Transcription/Translation Kit (Promega), diluted 1:1 in MEDMDG buffer (25 nM MOPS ...
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bioRxiv - Biochemistry 2021Quote: ... the TNT® Quick Coupled Transcription/Translation Kit from Promega was used according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: A rabbit reticulocyte lysate in vitro translation kit (Promega, L4960) was coupled to in vitro transcription with the supplementation of MgCl2 ...
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bioRxiv - Biochemistry 2023Quote: The TNT® Quick Coupled Transcription/Translation Kit from Promega was for synthesis of 35S-methionine labeled proteins in reticulocyte lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... and in vitro translated (Wheat Germ in vitro translation kit, Promega #L4380). The in vitro translated Zfp207-Halo fusion product was incubated with total RNA from mouse ESCs ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Cell Biology 2023Quote: ... 35S-methionine-labeled Ctb or its derivatives were produced in coupled in vitro transcription and translation reaction (IVTT, at 30 °C for 1 h) using TNT Quick Coupled Transcription/Translation System (cat#L1170, Promega, Madison, WI, USA). Prey proteins were diluted in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... In vitro translation was performed using a TNT SP6 Quick Coupled Transcription / Translation System (Promega, L2080). 40 μl of rabbit reticulocyte lysates supplemented with methionine were incubated with 1 μg of pKS103-xLIG1 ...
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bioRxiv - Immunology 2023Quote: In-vitro translation assay was performed using TNT® Quick Coupled Transcription/Translation System (Promega #L1170) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Microbiology 2019Quote: ... Translation of Firefly luciferase mRNA (Promega) was quantified using a Victor 2 microplate reader (PerkinElmer).
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bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
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bioRxiv - Bioengineering 2024Quote: Coupled transcription-translation assays were carried out using the TNT SP6 Quick Coupled transcription/translation system (Promega). Reactions were assembled containing 8 μl TnT Quick Master Mix ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Physiology 2020Quote: The in vitro translation analysis was performed using the TnT® Quick Coupled Transcription/Translation System from Promega by following the manufacture’s protocol ...
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bioRxiv - Microbiology 2022Quote: Coupled in vitro transcription and translation assays were performed using the TNT Quick Coupled Transcription/Translation system (Promega) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: Coupled in vitro transcription and translation assays were performed using the TNT Quick Coupled Transcription/Translation system (Promega) as described previously (16) ...
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bioRxiv - Molecular Biology 2024Quote: In vitro translation (IVT) of each eIF4E was performed using the TNT Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol and based on a protocol developed to study zebrafish eIF4E (111) ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Cancer Biology 2021Quote: ... T7 expression vectors were used in an in vitro translation reaction using TNT Quick Coupled Transcription/Translation System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pGEM-1 and the TNT SP6 Transcription/Translation System were from Promega. [35S]Met was from PerkinElmer.
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bioRxiv - Plant Biology 2019Quote: ... HA-tagged RPT2 N proteins were prepared with in vitro transcription and translation of pGADT7-RPT2 N using the TNT Quick Coupled Transcription/Translation Systems (Promega).
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bioRxiv - Immunology 2020Quote: Tle3 containing a flag tag in the C-terminus was cloned into the pTNT plasmid and transcription and translation was subsequently performed using the TNT Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... from designated translation reporter constructs were used for a 25 µL translation reaction volume using wheat germ extracts (WGE; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription and translation reactions using rabbit reticulocyte lysates as previously described (29) were performed by TNT Quick Coupled Transcription/Translation System (Promega) in the presence of [35S] Met (Perkin-Elmer) ...
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bioRxiv - Biochemistry 2022Quote: ... [35S]-labeled proteins were synthesized in vitro via a coupled transcription/translation rabbit reticulocyte kit (Promega) incubated at 30°C for 90 min with [35S]Met/Cys metabolic labeling reagent (MP Biomedical ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Molecular Biology 2019Quote: ... using a coupled transcription-translation system containing wheat germ extract (TNT kit; Promega, Cat no. L5030), according to the manufacturer protocol.
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bioRxiv - Molecular Biology 2023Quote: The TnT quick coupled transcription/translation system (Promega) was used to generate 35S-labeled eIF4B ...
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bioRxiv - Cell Biology 2023Quote: In-vitro transcription and translation (IVTT) reactions were performed using the SP6 TnT® Quick Coupled Transcription/Translation System (Promega #L2080) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... In vitro translation assays were run on the prepared mRNA templates using Rabbit Reticulocyte Lysate kits (Promega), with translation mixes prepared in triplicate as per manufacturer′s protocols (for S2D ...
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bioRxiv - Cell Biology 2022Quote: ... [35S]-methionine-labelled proteins were transcribed and translated using a TnT™ coupled transcription–translation kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... we used TNT Quick coupled transcription/Translation System (Promega) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Nuclease-treated rabbit reticulocyte lysate translation reactions (Promega L4960) were set up in a 384-well plate (Thermo Scientific 164610 ...
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bioRxiv - Biochemistry 2021Quote: ... the TnT Quick Coupled SP6 Transcription/Translation System (Promega) was used by following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: In vitro translation reactions were performed in WGE (Promega) as described [93] ...
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bioRxiv - Cell Biology 2020Quote: ... the FluoroTect− GreenLys in vitro Translation Labeling System (Promega) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... After the translation reaction was quenched by adding 30 μl of 1× Passive Lysis Buffer (Promega), 10 μl of the mixture was placed on a 96-well white assay plate (Coster) ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro transcription and translation to radiolabel the proteins with [35S]-methionine was performed with the TnT® Quick Coupled Transcription/Translation System (Promega, Walldorf, Germany) in reticulocyte lysate ...
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bioRxiv - Cell Biology 2019Quote: ... In vitro translated (IVT-) proteins were synthesized using a TNT quick-coupled transcription-translation kit (Promega, Madison, WI). For synthesizing the Pav and PavDEAD proteins ...
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bioRxiv - Cell Biology 2023Quote: 35S-radiolabeled Tom40 precursor proteins were synthesized in vitro using the TNT Quick Coupled Transcription/Translation kit (Promega). Transcripts for MCO6 encoding 3 additional N-terminal methionine residues for radiolabelling of the Mco6 protein which lacks internal methionine residues ...