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Citations for Promega :
551 - 600 of 708 citations for Human Apolipoprotein AI B Free Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Residual genomic DNA was then removed from each sample with 10 U of RNAse-free DNaseI (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was aspirated and gently resuspended in 500 μl of cold buffer (1x of cold Hanks’ Balanced Salt Solution HBSS, 1% nuclease-free BSA, 22.5 μl of RNasin Plus (Promega N2611) and 1/2000 DRAQ5).
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bioRxiv - Molecular Biology 2020Quote: ... using the TRIzol method (as described by the manufacturer) An aliquot of total RNA was treated with RQ1 RNase-free DNAse (Promega), to avoid genomic contamination and 1μl of total RNA was quantified by spectrophotometer using a Nanodrop 1000 (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Fresh batches of ARPE-19 cells from a contamination-free stock that had been tested for mycoplasma (MycoAlert; Promega Inc.) were used every 6 to 8 weeks ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA contaminations in the RNA samples were removed by DNase treatment according to the instructions provided by the manufacturer (RQ1 RNase free DNase, Promega). cDNA was synthesized from 2µg of DNA free RNA using MMLV reverse transcriptase and random primers (Promega) ...
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bioRxiv - Microbiology 2023Quote: Single colonies were collected with a 1μl loop and suspended in 20μl of nuclease free water (Promega, Madison WI, USA). The same 1μl loop was then used to streak a FAA + 10% DHB plate to subculture and isolate pure colonies ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of total RNA was incubated with RNase-free DNase I (1 unit/μl) following the protocol provided by the manufacturer (Promega), followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega) ...
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bioRxiv - Immunology 2023Quote: The specific activity of Caspase-1 was assessed in cell-free supernatants using Caspase-Glo® 1 Inflammasome Assay (Promega). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Trace amounts of contaminating genomic DNA were removed by treating all RNA samples with RNAse-free DNase I prior to use for cDNA synthesis according to the manufacturer’s instructions (Promega, WI).
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... emulsions of octanol + 5% (w/v) Span 80 + 40 µM Nile Red in an aqueous phase of nuclease free water (Promega) + 200 ng/µL miniprepped plasmid + 5% (w/v ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
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bioRxiv - Neuroscience 2020Quote: ... and pCMVΔ8.9 plasmids were co-introduced into human embryonic kidney 293T cells using the FuGENE reagent (Promega). 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... albicans to damage human vascular endothelial cells was assessed by the CytoTox-96 assay (Promega, Madison, WI), which measures the release of lactate dehydrogenase (LDH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected with human RXFP1 pcDNA-Zeo-tetO and the GloSensor reporter plasmid using FuGENE (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293T cells were co-transfected with human MOR and a luciferase-based cAMP biosensor (GloSensorTM, Promega). The next day ...
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bioRxiv - Genetics 2022Quote: We performed transient transfection on human pluripotent stem cells using FuGENE® HD Transfection Reagent (Promega, E2311). The cells were splitted and treated with Y-27632 for one before transfection.
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bioRxiv - Genomics 2023Quote: ... Primer efficiency was calculated using a 10-fold serial dilution of Human Mixed Genomic DNA (Promega G3041). For primer sequences and calculated efficiencies ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Cell Biology 2024Quote: All human cell lines were authenticated at each batch freezing by STR profiling (StemElite ID System, Promega). All cell lines were tested for mycoplasma at each batch freezing by PCR (32 ...
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bioRxiv - Cancer Biology 2019Quote: ... Bovine serum albumin and the Cell Titer-Glo Luminescent Cell Viability Assay Kit were purchased from Promega Corporation (Madison ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was eluted with 25 μl of RNase-free water and was quantified using the Quantifluor RNA System (Promega, #E3310).
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bioRxiv - Genomics 2021Quote: ... Total RNA was extracted from cultures in mid-log phase using a standard hot phenol method and treated with RNase-free DNase RQ1 (Promega, M6101) to remove any DNA contamination ...
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bioRxiv - Immunology 2021Quote: ... 2.5 μl TDE1 and 22 μl nuclease-free water were combined and placed at 37°C for 3 min before 0.5 μl of 1% Digitonin (Promega, Cat. #G9441) was added to the master mix ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated according to Chomczynski and Sacchi (Chomczynski and Sacchi, 2006) with integrated RNase free DNaseI (Promega, Madison, Wisconsin) treatment.
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bioRxiv - Microbiology 2021Quote: ... labeling was promptly carried out in an RNase-free environment in the presence of RNasin at 0.133 U/ml (Promega, Southampton; [49]).
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bioRxiv - Plant Biology 2022Quote: ... according to manufacturer’s instruction with on-column DNase digestion using 60 μL of DNase mixture (15 μL RQ1 RNase-Free DNase (Promega, M6101), 6 μL RQ1 DNase 10X Reaction Buffer and 19 μL nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... The chromatin pellet was incubated with 50 μl of 1X RQ1 DNase Reaction Buffer with 30U of RQ1 RNase-Free DNase (Promega, # M6101) for 10 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Zoology 2019Quote: ... Traces of genomic DNA were removed by treating 2 μg of the total RNA with RQ1 RNase-Free DNase (Promega, USA). First-strand cDNA was synthesised from RNA using the M-MLV reverse transcriptase (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: All wheat germ cell-free expression used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was again removed and replenished with 30 mL phenol-red free Opti-MEM supplemented with 5 mM CT-TAMRA (Promega, G8251), except for no CT-TAMRA control wells ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturer’s protocol with the modification of on column DNase digestion using RQ1 RNase free DNase (Promega, Madison, WI) in the enzyme mix ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were confirmed to be free of mycoplasma (PCR-based method) and authenticated by Short Tandem Repeat (STR) analysis using the GenePrint® 10 System (Promega, Madison ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
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bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Biophysics 2019Quote: The cell-viability of human neuroblastoma (SH-SY5Y) cells was measured using MTT cell proliferation assay (Promega, G4000). SH-SY5Y cells were plated in a 96-well plate followed by differentiation in Neurobasal-A ...
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bioRxiv - Molecular Biology 2019Quote: Full length 3’-UTR of human (P)RR and LDLR were cloned into the luciferase reporter vector (Promega). Mutant 3’-UTR luciferase reporter vectors were generated by PCR using site-directed mutagenesis technique ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
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bioRxiv - Genetics 2020Quote: ... cells were changed into GloSensor equilibration medium (10% fetal bovine serum and 2% GloSensor assay reagent (Promega E1290) in CO2-independent medium (Gibco 18045-088) ...
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bioRxiv - Genetics 2021Quote: ... and 50 million nuclei per liver were incubated in a volume of 950 ul with 32 U of RQ1 RNase-free DNase I (Promega, cat. # M6101) for 2 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was prepared from RV free wall and HD-area pieces using the Wizard® Genomic DNA Purification Kit (Promega, France). Samples were submitted to polymerase chain reaction analysis ...
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bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...