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Citations for Promega :
451 - 500 of 708 citations for Human Apolipoprotein AI B Free Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Carried over DNA was removed by off-column treatment with RQ1 RNase-free DNase (Promega cat# M610140). qPCR was done using an Applied Biosystems 7500 Fast Instrument with Quantitation -Standard Curve experimental type and Takyon Low ROX SYBR 2X MasterMix blue dTTP (Eurogentec cat# UF-LSMT-B0710 ...
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bioRxiv - Genetics 2020Quote: ... The microinjection reagents were combined in nuclease-free T10E0.1 pH 7.5 (Integrated DNA Technologies; Tris 10mM, EDTA 0.1mM) supplemented with Rnasin (Promega) at 0.2 U/ul ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μl of template and ultrapure DNAse/RNAse free distilled water (Promega Corporation, Fitchburg, WI, USA).
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Pathology 2020Quote: ... and contaminating DNA was removed by using the RNase-free RQ1 DNase (Promega Corp., Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA from each sample was treated with RQ1 RNase-Free DNase (Promega Co.) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Any contaminant genomic DNA was degraded by DNase treatment (RQ1, RNase-free DNase, Promega Corp., WI, USA). cDNA was generated using M-MLV reverse transcriptase (Promega Corp. ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 2µg of DNA free RNA using MMLV reverse transcriptase and random primers (Promega). Quantification of the cDNA was carried out in the Rotor gene 3000A machine (Corbett ...
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bioRxiv - Developmental Biology 2021Quote: ... DNase I treatment and cDNA synthesis were preformed using the RQ1 RNASE-FREE DNASE 1* (Promega®) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific®) ...
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bioRxiv - Microbiology 2022Quote: ... Residual genomic DNA was removed by treating with 10 units/sample of RNAse-free RQ1 DNAse (Promega) for 1h at 37°C followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was removed from the total RNA by treatment with RQ1 RNase-free DNase (Promega, M6101). cDNA synthesis was carried out with the RevertAid First Strand cDNA synthesis Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... of luciferase was determined using a PicaGene Luminescence Kit (TOYO B-Net Co., LTD, Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... The relative light unit value of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of DNAse free RNA was used for cDNA synthesis using ImProm-II Reverse transcription system (Promega), as per the manufacture’s recommendation ...
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bioRxiv - Synthetic Biology 2022Quote: ... the tRNA pellet was resuspended in water and incubated with 8 U of RQ1 RNAse-free DNAse (Promega) at 37 °C for 30 min according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Any residual genomic DNA was removed by treating RNA with RQ1 Rnase-free DNase (Promega, Madison, WI, USA) at 37°C for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Free UDP was measured as a proxy for reaction progress using the UDP-Glo enzyme assay kit (Promega) (Zegzouti et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 800 ng of total RNA were freed from genomic DNA using the RQ1 RNase-Free DNase Kit (Promega). Reverse transcription was performed using 145,4ng of the total DNase-treated RNA using the TaKaRa PrimeScript RT Master Mix Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Microbiology 2022Quote: ... Additional DNAse treatment was also performed on the harvested RNA using RQ1 RNase-Free DNase (Promega, catalog # M6101) according to the manufacturer’s protocol to remove any carryover of double-stranded or single-stranded DNA ...
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bioRxiv - Plant Biology 2022Quote: ... and the concentration was measured before and after DNase I digestion (RQ1 RNase free DNase I, Promega®) using spectrophotometry (OD260) ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 μL of cell-free supernatants were mixed with 50μL CellTiter-Glo Luminescent Cell Viability Assay (Promega, G7571) in white 96-well plates (Greiner ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed in antibiotic free medium using FuGENE® HD transfection reagent (Promega Corporation, Durham, NC, USA) following the manufacturer’s instructions with a final plas-mid amount of ∼100-200 ng per well ...
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bioRxiv - Bioengineering 2023Quote: ... RNA samples prepared were treated with DNase according to the manufacturer’s instructions (RQ1 RNase-Free DNase from Promega), quantified with a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... eluted in 12 µl of nuclease free water and quantify using the dsDNA QuantiFluor Dye System (Promega, #E2670).
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Molecular Biology 2020Quote: Cells were blocked by pipetting 50ul of 5% Bovine serum albumin (BSA) (Promega, Cat: W3841) directly onto slides ...
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bioRxiv - Genomics 2021Quote: The cffDNA was extracted from serum using a Maxwell RSC cffDNA Plasma Kit (Promega; AS1480) by the Primate Assay Laboratory Core at the California National Primate Research Center ...
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bioRxiv - Microbiology 2023Quote: ... The value of relative light unit of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI, USA), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... the infectivity of these pseudotyped viruses was determined by measuring luciferase activity using the PicaGene BrillianStar-LT Luciferase Assay System (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI, USA) or by observing EGFP expression under a fluorescence microscope (IX71 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was prepared using TRI reagent (MRC) according to the manufacturer’s instructions and treated with RQ1 RNAse-free DNase (Promega) and proteinase K (Sigma) ...