Labshake search
Citations for Promega :
351 - 400 of 624 citations for Histone H3 Rabbit Polyclonal HRP labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... cells were washed with HBSS and labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corp., Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1h with secondary antibodies (mouse antirabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by secondary antibodies conjugated with HRP or alkaline phosphatase (Promega, Madison, WI, 1:1,000). Proteins were visualized with a LAS-3000 mini (GE Healthcare ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by a 1:20,000 dilution of anti-mouse IgG HRP conjugated secondary antibody (Promega) for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... or secondary antibodies: anti-mouse IgG horseradish peroxidase (HRP) (Promega, Madison, WI, W402B, 1:10,000), anti-rabbit IgG HRP (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... membranes were washed and incubated with the appropriate HRP-conjugated secondary antibody (Promega, 1:3000) and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-GFP 1:2000 (MBL, RRID: AB_591819) rabbit anti-Halo 1:1000 (Promega, RRID: AB_713650), rat anti-HA 1:500 (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit rabbit IgG antibody (Promega #W4021 and #W4011) were used at 1:2,500 dilution followed by detection by ECL (SuperSignal West Pico Chemiluminescent Substrate ...
-
bioRxiv - Plant Biology 2020Quote: ... a rabbit-anti-mouse-IgG (Promega) coupled with an alkaline phosphatase was used.
-
bioRxiv - Cell Biology 2019Quote: ... and rabbit reticulocyte lysate system (Promega) respectively ...
-
bioRxiv - Biochemistry 2020Quote: The Rabbit Reticulocyte Lysate System (Promega) was used to determine the influence of internal modifications on translation efficiency of RNA F ...
-
bioRxiv - Biophysics 2023Quote: Rabbit reticulate lysate (RRL, L4960, Promega) is used as the artificial cytoplasm to fulfill the requirement of cytosolic factors necessary for nuclear transport (S ...
-
bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit IgG (Promega, #W4011). The membrane was washed three times with PBS for 10 minutes and subjected to signal detection using ECL™ Prime Western Blotting System (Cytiva ...
-
bioRxiv - Cancer Biology 2024Quote: Rabbit reticulocyte lysates (RRL) (Promega L4960) were treated with micrococcal nuclease (NEB M0247S ...
-
bioRxiv - Synthetic Biology 2021Quote: ... transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771). Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend ...
-
bioRxiv - Molecular Biology 2023Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5,000; 1:10,000; or 1:20,000; Promega, W4021) was used as the secondary antibody ...
-
bioRxiv - Neuroscience 2020Quote: ... digoxigenin (DIG)-labeled riboprobes were generated with a commercial transcription kit (Riboprobe System, P1440, Promega, San Luis Obispo, CA) and DIG RNA labeling mix (Roche REF# 11277073910 ...
-
bioRxiv - Biochemistry 2019Quote: ... Dynein (always as dimer and either labeled with GFP only or with GFP and a Halo488 dye (Promega, G1001)) was excited with a 488 nm laser (Coherent Sapphire 488 LP ...
-
bioRxiv - Biophysics 2022Quote: ... eGFP was purified and labeled with halotag Succinimidyl Ester (O4) ligand as previously described (Huang et al., 2017) (Promega).
-
bioRxiv - Microbiology 2023Quote: ... The FAM-labeled probes were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and quantified with a NanoDrop 2000C (Thermo ...
-
bioRxiv - Neuroscience 2023Quote: ... The antisense radio-labeled 35S-Vglut2 riboprobe was generated by in vitro transcription using a T7 RNA polymerase (Promega). 35S-labeled riboprobe was diluted in hybridization solution (50% formamide ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
-
bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
-
bioRxiv - Microbiology 2023Quote: ... and then incubated for another hour with an anti-Mouse IgG HRP-conjugate secondary antibody (Promega) (diluted 1:10 000 in PBST) ...
-
bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...
-
bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with an HRP-conjugated secondary antibody (Promega, Cat# W4021, 1:10,000 dilution) in blocking solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
-
bioRxiv - Developmental Biology 2019Quote: ... Species-specific digoxigenin (DIG) labeled riboprobes were generated by in vitro transcription of Crabp1 fragments cloned in pGEMT-Easy (Promega) vector ...
-
bioRxiv - Biochemistry 2020Quote: The two Munc13-Halo proteins were labeled by incubating the protein with Alexa 488 conjugated with HaloTag® from Promega, as described before (2) ...
-
bioRxiv - Cell Biology 2022Quote: ... Northern blot probes for the corresponding RNAi targeting fragment were radioactively labeled using the Prime-a-Gene labelling system (Promega).
-
bioRxiv - Cell Biology 2020Quote: DRGs neurons infected with lentivirus expressing Halo-SFPQ were grown in XonaChip microfluidic chambers and were labeled with tetramethyl Rhodamine (TMR) Halo Tag ligand (Promega) according to the manufacturer’s instructions with modifications ...
-
bioRxiv - Genetics 2020Quote: ... The DNA templates used for DIG-labeled STX18-AS1 RNA probe in vitro syntheses (Supplementary file) with T3 RNA polymerase (Promega) were amplified from the coding sequence of STX18-AS1 from human foetal hearts cDNA (obtained from HDBR) ...
-
bioRxiv - Cell Biology 2021Quote: ... Eluted protein was labeled with either 1.75 µM AlexaFluor 660 or 2.5 µM TMR HaloTag Ligands (Promega, G8471 and G8251) for 2 hr on ice ...
-
bioRxiv - Developmental Biology 2021Quote: ... using primers 1 and 2 (Supplementary Table) and used for making a Digoxigenin (DIG)-labeled FISH probe using T3 RNA polymerase (P2083, Promega). After T3 RNA polymerase reaction at 37°C for 4 hours ...
-
bioRxiv - Plant Biology 2022Quote: ... The primer and DNA marker were end-labeled with 10 μCi of [γ-32P]ATP by T4 polynucleotide kinase (Promega) and then used for reverse transcription with avian myeloblastosis virus (AMV ...
-
bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
-
bioRxiv - Molecular Biology 2023Quote: ... The digoxigenin-labeled antisense and sense sybu riboprobes were synthesized from a SacII-or SacI-linearized pGEM-T easy (Promega), which contained an 823 bp cDNA-fragment from sybu-mRNA (NCBI Reference Sequence ...
-
bioRxiv - Molecular Biology 2023Quote: ... siRNAs were detected with a YFP long probe (primers listed in Supplemental Table 2) labeled with 32P-dCTP prepared according to the manual of the Prime-a-Gene Labeling System (Promega). The blot was hybridized overnight at 42 °C with the probe before being washed with 2xSSC ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Lumit anti-rabbit antibody-SmBiT (Promega). Final concentration of each antibody is 0.15 μg ml−1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-Halotag (Promega, G9281, 1:500), rabbit anti-Sox2 (Abcam ...
-
bioRxiv - Molecular Biology 2020Quote: ... then incubated with rabbit or mouse (Promega) HRP-conjugated secondary antibody diluted 1:4000 in 5 % milk powder in TBST for 45 min and washed again ...
-
bioRxiv - Developmental Biology 2019Quote: ... Rabbit monoclonal anti-pJNK (1:100; Promega) or rat anti-Dilp8 (1:50 ...
-
bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-β-Galactosidase (1:1500; Promega), mouse anti-β-Galactosidase (40-1a ...
-
bioRxiv - Microbiology 2021Quote: ... a rabbit reticulocyte lysate (Promega, Cat: L4151) was thawed at 37°C immediately before use and supplemented with 40 μl of 1 mM hemin stock solution and 10 μl of 1 M creatine phosphate (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... Rabbit reticulocyte lysates were obtained from Promega. For each experimental replicate ...
-
bioRxiv - Plant Biology 2020Quote: ... anti-rabbit IgG horseradish peroxidase conjugate (Promega), anti-mouse IgG horseradish peroxidase conjugate (Promega) ...
-
bioRxiv - Biochemistry 2022Quote: ... or Goat anti-Rabbit IgG (W401B, Promega) secondary antibodies for 1 hour (h) ...
-
bioRxiv - Cancer Biology 2023Quote: ... or rabbit IgG (cat. no. W401B; Promega). Protein signals were visualized using an iBright CL1500 Imaging system (Invitrogen) ...
-
bioRxiv - Cancer Biology 2023Quote: ... the G9281 rabbit anti-HaloTag antibody (Promega) was used at a dilution in the range of 1:30 to 1:500 (1:500 is the working dilution for immunofluorescence).
-
bioRxiv - Cell Biology 2023Quote: ... anti-rabbit IgG (Promega, W4011, 1:5000) or anti-mouse IgG (Promega ...