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Citations for Promega :
551 - 600 of 624 citations for Histone H3 Rabbit Polyclonal HRP labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega). For in vitro translations ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by a secondary anti-rabbit IgG antibody conjugated to alkaline-phosphatase (Promega, Madison, WI, USA). The membranes were blocked in TBST (150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega, USA). Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
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bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
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bioRxiv - Cell Biology 2022Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) used the SP64 vector as a backbone (Promega). For in vitro insertion reactions ...
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant and pellet fractions were spotted onto nitrocellulose and blotted with rabbit anti-HaloTag pAB (Promega). For microscopic examination ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted 1:10.000 in PBS-T with 3% milk powder for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... bound antibodies were revealed with a peroxidase-linked secondary anti-rabbit antibody (Promega, Madison, WI, USA) and visualized by chemiluminescence (ImmunoStar Zeta ...
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bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) or wheat germ (WG) extracts (Promega). Typical reactions were composed of 90% v/v RRL ...
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bioRxiv - Bioengineering 2021Quote: Target proteins were in-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies: mouse anti-β-tubulin (E7, Developmental Studies Hybridoma Bank) and rabbit anti-Halo (G9281, Promega). Secondary antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... Immuno-signals were visualized with an alkaline-phosphatase (AP)-conjugated anti-rabbit IgG antibody (1:5000, Promega). Western blot revelation was performed using a cheminoluminescence digital imaging system (ImageQuant Las-4000 Mini ...
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bioRxiv - Biochemistry 2020Quote: ... In vitro translation assays were run on the prepared mRNA templates using Rabbit Reticulocyte Lysate kits (Promega), with translation mixes prepared in triplicate as per manufacturer′s protocols (for S2D ...
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bioRxiv - Cell Biology 2022Quote: Constructs for in vitro translations in rabbit reticulocyte lysate were based on the pSP64 vector (Promega, USA). Constructs for in vitro translation in the E ...
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bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
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bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Molecular Biology 2020Quote: β-globin 48S IC and H4 48S IC were isolated from nuclease-treated rabbit reticulocyte lysate (RRL) (Promega L4960), as previously described (Simonetti et al. ...
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bioRxiv - Microbiology 2021Quote: In vitro transcription and translation reactions were conducted using the TNT coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 1:10,000 for WB) or goat-anti-rabbit IgG (W4011, 1:10,000 for WB) were purchased from Promega. APC anti-human CD63 (353008 ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Cell Biology 2019Quote: Target proteins were in vitro transcribed and translated in rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with 35S-Methionine (IsoLabel L-35S Steady Blue ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
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bioRxiv - Molecular Biology 2020Quote: MC Stem-HalV and GUS-(HalV IGR) mRNAs (3 pmol) were translated in 20μl reaction volume of Flexi rabbit reticulocyte lysate (Promega) in the presence of [35S]methionine (>37.0 TBq/mmol ...
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bioRxiv - Plant Biology 2020Quote: ... We found that using rabbit reticulocyte (TnT® T7 Coupled Reticulocyte Lysate System, Recombinant RNasin® Ribonuclease Inhibitor- Promega) instead of wheat germ extract for in vitro transcription and translation of the ABFs significantly reduces the background noise and increased the quality of peaks that are able to be pulled down from DAP-Seq ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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bioRxiv - Molecular Biology 2022Quote: ... The mRNA at a concentration of 214 ng/µL was translated in the Flexi Rabbit Reticulocyte Lysate System (Promega) for 25 minutes at 32°C resulting in run-off ribosome nascent chain complex (RNC) ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary goat anti-mouse and anti-rabbit antibodies conjugated with alkaline phosphatase used for Western blots were from Promega.
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bioRxiv - Microbiology 2023Quote: ... in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega) in TBS-T ...
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bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
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bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
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bioRxiv - Neuroscience 2020Quote: In vitro translation rates represent the increase in luminescence as a function of time using a standard rabbit reticulocyte lysate system (Promega) with luciferase mRNA (Promega) ...
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bioRxiv - Microbiology 2019Quote: In vitro translation reactions were performed using coupled bacteriophage T7 RNA polymerase transcription-rabbit reticulocyte lysate translation reactions as per the manufacturer’s instructions (Promega TNT). Samples were radiolabelled with 35S-methionine (Perkin Elmer ...
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bioRxiv - Biochemistry 2020Quote: All basic transcription-translation reactions for PrP constructs were performed using a Rabbit Reticulocyte Lysate (RRL) derived SP6 coupled system (Promega) for 30 min at 30 °C in the presence of 2.5 μCi [35S]-methionine in a total volume of 10 μl ...
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bioRxiv - Plant Biology 2020Quote: ... The target proteins were immunodecorated with primary antibodies and then incubated with horseradish peroxidase-conjugated anti-rabbit IgG antibodies (Catalogue number: W4011, Promega) at a dilution ratio of 1:20.000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison ...
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bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
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bioRxiv - Bioengineering 2020Quote: ... Cas13a mRNA was translated in vitro using the Rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, WI, USA). RNaseAlert®-1 Substrate was mixed with crRNA or NTCR (500 nM ...
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bioRxiv - Immunology 2019Quote: 8 nM cap0 or 20 ng/μL VPg-linked RNA was translated using the Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence or absence of 1.5 μM Ifit proteins ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Genomics 2019Quote: To generate recombinant p53 we in vitro transcribed/translated human p53 with a c-terminal HA tag using a rabbit reticulocyte system (Promega). To generate fragmented genomic DNA we tagmented 50ng of human genomic DNA from MCF7 cells using the MuSeq kit (Thermo ...