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Citations for Promega :
101 - 150 of 2832 citations for Glucosamine Phosphate N Acetyltransferase 1 GNPNAT1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunoblotting: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:1,000 dilution), mouse monoclonal antibody to lamin A/C (Cell Signalling ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were revealed using species-appropriate HRP-conjugated secondary antibodies (anti-rabbit or anti-mouse IgG (1:5000, Promega), or anti-guinea pig IgG (1:5000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with NOCT N-terminal mutant constructs using Fugene HD (Promega) in a ratio of 3 µL transfection reagent per 1 µg DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... HiBiT fusion vectors were constructed using the parental vector pBiT3.1-N (N2361, Promega). To generate the HiBiT-VHL vector ...
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bioRxiv - Molecular Biology 2023Quote: ... NanoLuc fusion vectors were constructed using the parental vectors pNLF1-N (N1351, Promega) and pNLF1-C (N1361 ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of rabbit polyclonal antibody against PLRV-CP (1:5000; Promega) was added to each well and incubated for 3 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to horseradish peroxidase (1:10,000, W401B and W402B, Promega) were applied in the same solution for one hour at room temperature before activity was detected (SuperSignal West Pico PLUS 34580 ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase-conjugated (HRP) secondary rabbit antibodies (Promega, 1/25000) and revealed by chemiluminescence (Pierce ...
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bioRxiv - Biochemistry 2022Quote: ... membrane was incubated with anti-HaloTag antibody (1:1000; Promega Corporation; G9281) and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibodies: anti-rabbit HRP or anti-mouse HRP (Promega, 1:10000).
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies used were: anti-rabbit-IgG-HRP (W401B, 1:8000, Promega); anti-mouse-IgG-HRP (W402B ...
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bioRxiv - Cell Biology 2024Quote: ... followed by HRP-conjugated secondary anti-mouse antibody (1:5000, Promega W402B), diluted as indicated ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated for 1 hour at room temperature with an HRP-conjugated secondary antibody (1:1000, Promega). Protein expression was revealed using the Clarity kit (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: ... For immunostaining of the induced neurons following antibodies were used: anti-Tuj 1 (mouse; 1:2000, G7121, Promega), anti-MAP2 (chicken ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included goat anti-rabbit IgG HRP-conjugated antibody (1:5,000, #7074S, CST) and goat/rabbit/donkey anti-mouse IgG HRP Conjugate (1:5,000, #W402B, Promega). The membranes for streptavidin blotting were blocked with 3% BSA ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunofluorescence staining: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:500 dilution), mouse monoclonal antibody to lamin A/C (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: N-terminal NanoLuc/Kinase fusions were encoded in pFN31K or pFC32K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
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bioRxiv - Biochemistry 2022Quote: ... N-or C-terminal NanoLuc/Kinase fusions were encoded in pFN31K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
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bioRxiv - Biophysics 2021Quote: ... The primer sequence of SmBiT was designed from pBiT2.1-N [TK/SmBiT] Vector (Promega).
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bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
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bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... were made using the NanoBRET™ PPI MCS Starter System (Promega, cat n° N1811). The RRM2 fragments were amplified by PCR using Phusion polymerase (NEB ...
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bioRxiv - Immunology 2020Quote: ... 1:3,000 dilution of HRP-conjugated anti-mouse IgG secondary antibody (W402B, Promega) was added and incubated for one additional hour ...
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bioRxiv - Microbiology 2021Quote: ... Immunodetection was carried out using rabbit polyclonal anti-Halo antibody (Promega; 1:1000), mouse anti-HA antibody (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... The primary antibodies that were used are mouse β-Gal (1:250; Promega), mouse anti-Cora C615.16 (1:400 ...
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bioRxiv - Developmental Biology 2021Quote: The antibodies used were: Rabbit anti-pJNK pTPpY (1:500, Promega, Cat#V93B), Rat anti-Ci(1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Secondary anti-mouse IgG HRP antibody was used at 1:7000 dilution (Promega). ChemiDoc (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit polyclonal anti-cleaved Caspase 3 (1:200 dilution ...
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bioRxiv - Genomics 2024Quote: ... Membranes were then incubated with 1:10000 HRP-conjugated secondary antibody (Promega #W402B) in TBST + 5% milk for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody against HaloTag® (G9211; 1:1000) was purchased from Promega (UK).
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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bioRxiv - Neuroscience 2021Quote: Neuronal transfections were performed on DIV 7 using a calcium phosphate kit (ProFection Mammalian Transfection System, Cat # E1200, Promega), based on a previously described method (Sando et al. ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice with phosphate-buffered saline (PBS) and lysed with Luciferase Cell Culture Lysis 5x reagent (Promega). NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed with phosphate-buffered saline (50 mM sodium phosphate buffer, pH 7.0, 150 mM NaCl) and lysed in Reporter Lysis buffer (Promega). Luciferase activity was measured using a Luciferase Assay kit (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed twice with 1X phosphate-buffered saline (PBS) and lysed using 1X passive lysis buffer (Promega). The luciferase activity was measured using the dual luciferase assay kit (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG, HRP conjugate W402B, Promega; 1/5000 in TBST, 4 hours). As a loading control we used anti-tubulinα antibodies as primary antibodies (1/1000 in 5 % milk over-night) ...
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bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
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bioRxiv - Cell Biology 2019Quote: ... RT) and sequentially digested with Lys-C (Wako) (o/n at RT) and trypsin (Promega) (6 h at 37 °C) ...
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bioRxiv - Developmental Biology 2024Quote: ... Kanamycin-resistant colonies were selected and cultured o/n purified with Plasmid Miniprep system (Promega) and verified by sequencing (Microsynth ...
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bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...