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Citations for Promega :
1 - 50 of 2832 citations for Glucosamine Phosphate N Acetyltransferase 1 GNPNAT1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Plasmids used as DNA template in PCR reactions to generate donor DNAs for gene deletion/disruption strategies were obtained by amplifying blasticidin S deaminase (BSD) and puromycin-N-acetyltransferase (PAC) genes and cloning them individually into pGEM®-T Easy Vector (Promega). The reverse primers to amplify both genes included GTGA as four last nucleotides ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL Tris-phosphate buffer containing 1:50 Nanoglo substrate (Promega) was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day membranes were washed 3 times n TBTS for 5 minutes and incubated in secondary antibody (1:2500 anti-Rabbit HRP conjugated, Promega) diluted in blocking buffer for 1 hour at room temperature and washed 3 times with TBTS for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies used for western blotting were: anti-HALO (1:1000 in 0.5% milk TBST, 4°C o/n, Promega G9211), anti-GAPDH (1:10000 in TBST ...
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bioRxiv - Microbiology 2021Quote: ... and pVSV-G (4:3:1 ratio) using calcium phosphate transfection (Promega) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... or calcium phosphate (Promega). Cells were incubated for 24-48-hrs post-transfection before use.
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bioRxiv - Cell Biology 2021Quote: ... or Calcium Phosphate (Promega, E1200). Samples were prepared for analysis the day after DNA transfection.
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bioRxiv - Cell Biology 2020Quote: ... or Calcium Phosphate (Promega, E1200). Samples were prepared for analysis the day after DNA transfection ...
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bioRxiv - Cell Biology 2023Quote: ... or Calcium Phosphate (Promega, E1200). Samples were prepared for analysis the day after DNA transfection.
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bioRxiv - Genetics 2020Quote: ... cells were washed with 1× phosphate-buffered saline (PBS) then lysed with 1× passive lysis buffer (E194A, Promega). Cell lysates were assayed in triplicate in a 96-well plate and luciferase activity was quantified using a GloMax microplate luminometer (Promega) ...
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bioRxiv - Biochemistry 2020Quote: N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al. ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were enzymatic released using 3.5 U Flavobacterium meningosepticum N-glycosidase F (Promega) in 20µl water/well ...
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bioRxiv - Cell Biology 2021Quote: HEK cells were transfected with calcium phosphate (Promega, Profection Mammalian Transfection System) ...
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bioRxiv - Cancer Biology 2022Quote: Calcium phosphate (ProFection E1200, Promega Corp., Madison, WI) was used for plasmid transfection into HEK293T cells ...
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bioRxiv - Molecular Biology 2022Quote: ... pNLF1-N [CMV Hygro] (Promega), and pNLF1-C [CMV Hygro] (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted individually from whole bees (test; n=300, control; n=165) by Promega Wizard® purification.
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... An N-terminal HALO tag (Promega) for fluorescent labelling was fused to the coding sequence through a GGSGGSLQ linker ...
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bioRxiv - Neuroscience 2023Quote: ... and pBiT2.1-N [TK SmBiT] (Promega) vectors ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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bioRxiv - Molecular Biology 2019Quote: ... transfected cells growing in 12-well plates were carefully washed twice with warm phosphate-buffered saline (PBS) followed by treatment with 120 µl 1× Passive Lysis Buffer (PLB, Promega) by shaking for 10-15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and pNLF1-N-CMV-Hygro (Promega, N1351) were obtained from Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing grade trypsin (Promega, P/N V511A) was used for the generation of tryptic peptides.
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... and envelope plasmid using calcium phosphate-mediated transfection method (Promega, E1200) and harvesting the supernatant 48 - 72 h after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: The N protein of SARS-CoV-2 B.1 D614G variant (NYI67-20 strain) was cloned into Nano-Luc vector pNLF1-N [CMV/Hygro] (N terminus) (Cat. N1351, Promega) as a fusion protein ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Genomics 2020Quote: ... and unmethylated lambda DNA (Promega, Cat. N: D1521) was added to the samples (∼ 0.01%) ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Competent Cells, Promega, cat. n° L2005) and recombinant clones were checked by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cancer Biology 2022Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Immunology 2023Quote: ... and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH) in a ratio of 1:50 and O/N with 0.1 µg/µl trypsin (Promega) in a ratio of 1:50 ...
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bioRxiv - Immunology 2019Quote: ... Secondary antibody (α-Goat HRP, Promega, 1:10,000). The blot was developed using ECL Western Blotting Substrate (Promega ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 6228 and Uniport ID: P62266) and its N-terminally truncated mutant (deleted residues 1-41) were cloned into pEGFP-N1 vector (Promega) between KpnI and XhoI sites as described in Ref.19 ...
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bioRxiv - Microbiology 2019Quote: ... In-gel protein digestion was performed by O/N incubation of the gel pieces with 1 µg of sequencing grade modified trypsin (Promega) in 67 mM ammonium bicarbonate pH 8 at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Neuroscience 2020Quote: ... Trypsin Gold was obtained from Promega (Cat. N° V5280) (Madison ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 20 μl Stop n Glo substrate (Promega) diluted 1:1 with filtered dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... Asp-N (V1621) and Chymotrypsin (V1091) from Promega (USA) and Lys-C (125-05061 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Cell Biology 2019Quote: ... and into N’3xGFP and N’Halo (Promega, Madison, WI) pCS2+ with BspE1 and Xho1 ...
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bioRxiv - Genomics 2020Quote: ... 0.1% RNAsin plus RNase inhibitor (Promega, Cat. N: N261A) in PBS (wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and Asp-N were purchased from Promega (Madison, WI), and a-lytic protease was obtained from New England BioLabs (Ipswich ...
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bioRxiv - Developmental Biology 2021Quote: ... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
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bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...