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Citations for Promega :
401 - 450 of 1466 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 4 µl 5x reaction buffer (Promega, M289A), 2.4 µl MgCl2 (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 µl 5x reaction buffer (Promega, M289A), 2.4 µl MgCl2 (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 ng of Renilla control plasmid (Promega) and 0.62 μL of Lipofectamine 2000 Transfection Reagent (InvitrogenTM) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 4 μl of FuGENE 6 (Promega) in 100 μl of OPTI-DMEM ...
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bioRxiv - Genetics 2021Quote: ... total RNA was extracted from ∼25 mg of powdered right brain hemisphere tissue using Maxwell 16 Tissue LEV Total RNA Purification Kit (AS1220, Promega, Madison, WI). UNC HTSF core performed RNA concentration and quality check using fluorometry (Qubit 2.0 Fluorometer ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from faecal samples not stored in RNAlater® using the Maxwell 16 LEV Blood DNA kit (AS1290, Promega, UK) and automated DNA extraction system ...
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bioRxiv - Cell Biology 2022Quote: ... or SB431542 (1µM) (ALK5 kinase inhibitor) and relative luciferase activities quantified after 16 hours using the Dual-Glo® Luciferase Assay System (Promega®). Histograms were generated and data analyzed by multiple paired t-tests and 2-way ANOVA tests using GraphPad (PRISM® 9.0.0).
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bioRxiv - Cell Biology 2022Quote: ... or combined to K02288 at 1µM (ALK1 kinase inhibitor and relative luciferase activities quantified after 16 hours using the Dual-Glo® Luciferase Assay System (Promega®). The TGFβ Reporter Assay was performed in TMLCs after transfection of the various PTPN14 constructs ...
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bioRxiv - Physiology 2022Quote: ... DNA was isolated from snap-frozen ACA tissues with the Maxwell® 16 Tissue DNA Purification Kit (#AS1030, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from approximately 106 cells via the Maxwell 16 LEV simply RNA Tissue Kit (Cat. #AS1280, Promega, Madison, WI) on a Maxwell 16 Instrument (Cat ...
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bioRxiv - Systems Biology 2019Quote: ... was added to 800 µl of Opti-MEM with GlutaMAX supplement along with 16 µl FuGENE® HD Transfection Reagent (Promega Corporation), incubated for 15-30 minutes in a biosafety cabinet ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: Viral RNA was isolated from plasma samples using the Maxwell 16 Viral Total Nucleic Acid Purification kit on the Maxwell 16MDx instrument (Promega, Madison WI). Viral RNA was then quantified using a highly sensitive qRT-PCR assay based on a previously published protocol115.
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bioRxiv - Microbiology 2021Quote: ... The qPCR for 16S rRNA was carried out with a reaction mixture containing 10.5 µL GoTaq® qPCR Master Mix (2X) (Promega, Madison, USA), 0.2 µM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted using the Maxwell 16 LEV simplyRNA tissue kit in combination with the Maxwell nucleic acid extraction robot (Promega, WI, USA), according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were fingerprinted by the LCCC Tissue Culture and Biobanking Shared Resource (TCBSR) to verify their authenticity using the PowerPlex® 16 HS System (Promega, USA). The 42R cells are documented to be of the same origin as their parental cell line ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrates were washed thrice with 50 mM PB by centrifugation at 16 000 g and digested with sequencing grade Trypsin-Gold (Promega, Madison, USA) at the ratio of 1:30 (enzyme/protein ...
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bioRxiv - Plant Biology 2022Quote: ... and alkylation treatment (100 mM iodoacetamide / 45 min incubation at room temperature) were followed by 16 hr incubation at 37°C with sequencing grade trypsin (Promega, Madison WI). Tryptic peptides were acidified with formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Biophysics 2020Quote: ... 2 µL FluoroTect GreenLys (Promega), and 375 ng of plasmid encoding the protein of interest ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μg LysC (Promega). After digestion ...
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bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
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bioRxiv - Systems Biology 2021Quote: ... 2 µg of AspN (Promega), or 2 µg of GluC (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... 2 µL RNAse.In (Promega #N2115), 8 pmol of DNA from step 1 and 10 µL T7 HiScribe enzyme ...
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bioRxiv - Genomics 2019Quote: ... 2 µL RNAse.In (Promega #N2115) and 7 µL TGIRTIII enzyme (InGex) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 μL RNAse.In (Promega #N2115), 20 μL RNAse-free H2O ...
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bioRxiv - Biochemistry 2021Quote: ... 2 U/μL RNasin (Promega), 1 μM vRNA or cRNA promoter ...
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bioRxiv - Genomics 2023Quote: ... 2 µL RQ1 DNase (Promega) was added to remove templates and incubated at 37°C for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: The psiCHECK-2 plasmid (Promega) has a special NheI restriction site that was used to insert a synthetic DNA duplex encoding the G-rich region of the PRCC-TFE3 fusion gene in the upstream of the renilla luciferase initiation codon to create the plasmids G4Q27 and G4M27 (mutated G-rich region of the PRCC-TFE3 fusion gene) ...
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bioRxiv - Molecular Biology 2023Quote: ... psiCHECK-2™ (Promega, C8021) luciferase plasmids and primers used can be found in Supplemental Table S6B and Supplemental Table S7C ...
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bioRxiv - Microbiology 2021Quote: ... Digested protein suspensions with 4 μg trypsin (Promega) in 40 μL 25 mM NH4HCO3 buffer at 37 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... 4 U RNasin Ribonuclease Inhibitor (Promega, Cat#N2115), 6 U Recombinant RNase Inhibitor (Takara ...
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bioRxiv - Developmental Biology 2024Quote: ... and 4 μL of RQ1 DNase (Promega, M6101) were applied to the lysate and incubated at 37 °C for 3 min ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was prepared from a single colony cultured overnight at 37°C in LB using the Maxwell 16 system (Promega Corp., Madison, WI). Libraries for Illumina sequencing were prepared using either Nextera XT (Illumina ...
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bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
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bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
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bioRxiv - Microbiology 2020Quote: ... dahliae strain JR2 was isolated from tomato roots seven days after root dip inoculation and following five days of in vitro growth in soil extract and potato dextrose broth (PDB) using the Maxwell® 16 LEV Plant RNA Kit (Promega, Madison, USA). Real-time PCR was performed as described previously17 to determine the expression of effector genes relative to VdGAPDH with primer pairs as shown in Extended data Table 6.
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bioRxiv - Physiology 2020Quote: ... 500 ng of RNA were reverse-transcribed into cDNA using oligo(dT)16 and ImProm-II TM Reverse Transcriptase (Promega Corp., Madison, USA). cDNA products were amplified on a GeneAmp 7500 Fast System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from FFPE tumor tissue samples of 18 patients within the cohort using the automated Maxwell system with the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from FFPE tissue samples using the automated Maxwell system with the Maxwell 16 LEV RNA FFPE Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The corresponding Firefly luciferase (LUC) and Renilla luciferase (REN) values were measured 16 h after transfection using a Dual-Luciferase® Reporter Assay System (Promega, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... DNA was purified from the samples using the Maxwell 16 Tissue LEV Total RNA purification Kit custom-adapted for DNA extraction (cat no. AS 1220, Promega, Madison, Wisconsin, USA) in a Maxwell 16 MDx instrument (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: gDNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sections was carried out using Maxwell 16 FFPE plus LEV DNA purification kit (AS1720; Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... senegalensis DNA was extracted from muscle tissues in thoraxes using Maxwell® 16 LEV Plant DNA Kit (Cat. No. AS1420, Promega, WI, USA). Details on extraction and library preparation protocols are provided in the Supporting Text 1.
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bioRxiv - Biochemistry 2022Quote: 2’-Deoxythymidine-5’-triphosphate (dTTP) and 2’-deoxyguanosine-5’-triphosphate (dGTP) were obtained from Promega. 2-14C labeled 2’-deoxythymidine-5’-triphosphate (2-14C-dTTP ...