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Citations for Promega :
301 - 350 of 1466 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Total RNA from colon tissues was isolated using Maxwell® 16 LEV simplyRNA tissue kits (Promega, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA extraction was performed on the frozen cores using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, AS1280). Specimens were prepared for RNA-seq as described above and RNA quality was verified using Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Cell Biology 2019Quote: ... 4 μg (Promega, Cat. # v5113) was added to digest proteins to generate peptides overnight at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
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bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
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bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
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bioRxiv - Microbiology 2021Quote: ... 4 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
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bioRxiv - Microbiology 2023Quote: ... 4 mM magnesium chloride (Promega), 0.4 mM dNTPs (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl2 or 16% PEG and assayed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR was performed with 16 ng of cDNA per reaction with GoTaq qPCR Master Mix (Promega) and the CFX96 Touch Real-Time PCR Detection System (Biorad) ...
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bioRxiv - Bioengineering 2020Quote: ... The transduction efficiency was quantified at 16 h posttransduction by measuring firefly luciferase activity according to the manufacturer’s instructions (Promega).
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bioRxiv - Plant Biology 2020Quote: ... total RNA was extracted using Maxwell® RSC Plant RNA or Maxwell® 16 miRNA Tissue Kit from Promega according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and all subsequent steps were done as described in the manufacturer’s protocol (Maxwell 16 FFS Nucleic Acid Extraction System, Promega). DNA was eluted into a total volume of 80 μl elution buffer (EB ...
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bioRxiv - Systems Biology 2020Quote: ... Cell line identities were confirmed by STR fingerprinting by MD Anderson’s Cytogenetics and Cell Authentication Core (Powerplex 16 Locus High Sensitivity Assay, Promega).
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bioRxiv - Systems Biology 2021Quote: ... Protein digestion was performed for 16 h at 37 °C with 0.1 µg of Trypsin/Lys-C mix (1 µL of stock; Promega) and stopped by lowering pH to 3 by the addition of 5 µL of 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction from FFPE specimens of chRCC was performed using the Maxwell® 16 Tissue DNA Purification Kit (Promega). RNA quality was assessed with the RNA Qubit™ RNA HS Assay Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in 0.1M Triethylammonium bicarbonate (TEAB) and overnight (∼16 h) digestion was performed with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C with constant mixing using a thermomixer ...
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bioRxiv - Epidemiology 2019Quote: ... The 16S rDNA touchdown PCR was performed in 20 µL reactions containing 1× GoTaq® Green Mastermix (Promega, USA), 0.4 µM of each primer SpirRNAF and SpirRNAR (S2 Table ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted from zebrafish brain and intestine samples using an automatic system (Maxwell® 16 Instrument, Promega) and a total RNA purification kit (Maxwell® 16 LEV simplyRNA Tissue) ...
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bioRxiv - Bioengineering 2019Quote: ... Ligations of the digested plasmid backbones and PCR products occurred overnight at 16°C using T4 DNA ligase (Promega) prior to transformation into chemically competent Novablue E ...
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bioRxiv - Genomics 2021Quote: ... ligation was performed at 15°C for 16 hours using 100 ng of the digested DNAs and 4.5 U of T4 DNA ligase (Promega) in a final volume of 100 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested for 2 h at RT using 0.5 μg Lys-C (Wako Chemicals) and for 16 h at 37 °C using 1 μg trypsin (Promega). After centrifugation (10 min at 14 000 g) ...
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bioRxiv - Microbiology 2023Quote: ... USA) was performed using primer 27F on a V1-V3 16S rRNA gene colony-PCR amplicon (GoTaq Green, Promega) of primers 27F and 519R ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from FFPE samples (Discovery Cohort) using Maxwell 16 LEV RNA FFPE Kit (Promega, Madrid, Spain), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 2) Thermolysin (Promega) or 3 ...
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bioRxiv - Microbiology 2021Quote: ... (2) Trypsin (Promega), 4 h ...
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bioRxiv - Cell Biology 2022Quote: ... Erk1/2 (Promega), phospho-Erk1/2 (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μg of RNase A (Promega), and 40 units of RNase ONE (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
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bioRxiv - Genetics 2022Quote: ... instrument was configured with the Standard Elution Volume (SEV) hardware and using the Maxwell 16 Cell DNA purification kit (Promega). For total RNA isolation ...
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bioRxiv - Molecular Biology 2021Quote: Viral RNA was extracted from 0.2 mL of cobas PCR Media using Maxwell® 16 instrument (Promega, Madison, WI, USA) for final elution in 30μL ...
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bioRxiv - Microbiology 2020Quote: ... Infection rates were assessed by measuring luciferase activity in cell lysates at 16 h post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Systems Biology 2019Quote: ... The sample was digested for 16 h at 37C using a 1:50 (w/w) ratio of Sequencing Grade Modified Trypsin (Promega):protein in the gel ...
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bioRxiv - Microbiology 2019Quote: DNA from fecal samples and a blank control were isolated with the use of the Maxwell 16 Instrument (Promega Corporation) following the manufacturer’s instructions for the low elution volume blood DNA system ...
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bioRxiv - Biochemistry 2020Quote: ... three times with 250 μl of 50mM ammonium bicarbonate (ABC) buffer and digested for 16 hours with sequencing-grade modified trypsin (Promega) in 200 μl ABC buffer at 37 °C (enzyme to protein ratio 1:50) ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from nasopharyngeal swab samples using Maxwell® 16 Viral Total Nucleic Acid Purification Kit (Promega, Wisconsin-USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Systems Biology 2020Quote: ... pH 8.0 and digested for 16 hours on a shaker at 37 °C with a 1:40 ratio of Trypsin/Lys-C mix (Promega). Each sample was de-salted using HLB plates (Oasis HLB 30μm ...
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bioRxiv - Microbiology 2019Quote: ... Transient transfections were carried out for 16-20h with either 500 ng of construct DNA + 500 ng of pSFFV as filler using FugeneHD (Promega) or 1 µg of construct DNA using Polyjet transfection reagent (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega). For gRNAs utilized in in vitro cleavage reactions containing SpRY from human cell lysates ...
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bioRxiv - Cancer Biology 2022Quote: ... HCT116 cell line was treated for 24 h with 10 µM curcumin and RNA was isolated using the Maxwell® 16 IVD system and the Maxwell Simply RNA Tissue Kit according to the manufacturer’s instructions (Promega). RNA was reverse transcribed using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured 16 h post-infection using the Bright-Glo Luciferase Assay System or ONE-Glo Luciferase Assay System (Promega) and Centro xS960 luminometer (Berthold).
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from 300 µl plasma as previously described with a Maxwell 16 MDx instrument (Promega, Madison, WI) and evaluated using qRT-PCR (42) ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Table S4).
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene of each isolate was amplified from its genomic DNA using GoTaq G2 Hot Start Master Mix (Promega) and the purified PCR products were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit according to a previous report [15].
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bioRxiv - Genetics 2020Quote: The frameshifting efficiency of the reporter plasmids in cultured cells were assayed as described previously (15, 16) using a dual luciferase reporter assay system kit (Promega). 24 hours post transfection ...
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bioRxiv - Microbiology 2021Quote: ... Profiling of cell lines was performed using highly-polymorphic short tandem repeat loci (STRs) and amplification using PowerPlex 16 HS System (Promega), followed by fragment analysison an ABI3730xl (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from about 35 mg of tissue using the Maxwell™ 16 Tissue DNA purification kit (Promega) and total DNA concentrations were normalized to 20 ng/µl ...
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bioRxiv - Immunology 2020Quote: ... Total DNA from blood and urine (around 50 μL) was extracted using a Maxwell 16 automat with the Maxwell blood DNA and cell LEV DNA purification kits (Promega), respectively ...