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Citations for Promega :
601 - 650 of 6179 citations for Endothelin 1 ET 1 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-chicken IgY (1:1,000; G1351, Promega) and goat anti-mouse IgG (1:5,000 ...
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bioRxiv - Biophysics 2022Quote: ... 2 μg trypsin (protein:enzyme ratio 50:1; Promega) were added and proteins were digested at 37 °C for 18 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1:400 RNase inhibitor (Promega cat. #: N251B). Protein lysates were serially diluted to concentrations of 0.6 μg/μL ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse monoclonal antibIII tubulin (1:500, Promega, #G712A), mouse anti-tubulin bIII (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted Fz (1/40; Promega, Madison, WI, USA) in 200 µL 0.22 µm-filtered PBS was IV-injected to the mice via the tail vein for IVIS imaging ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 μg of trypsin/Lys-C (Promega) on a rotisserie for 18 h at ambient temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-beta galactosidase (Promega Z378825580610; 1:500), guinea pig anti-brk (provided by from Gines Morata ...
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bioRxiv - Biochemistry 2023Quote: ... and cloned into the plasmid pGEM-1 (Promega) as previously described (19) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of sequencing grade trypsin (Promega, V5111), diluted in 50 mM ammonium bicarbonate ...
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bioRxiv - Bioengineering 2023Quote: ... 1:200 RNasin® Ribonuclease Inhibitor (Promega, #N2615), 1x cOmplete™ ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µl of M-MLV Reverse Transcriptase (Promega) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μl Oligo-dT 500 μg/ml (Promega), 0.2 μl Random hexamers 500 μg/ml (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 μg mL-1 Proteinase K (Promega V3021), both ...
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bioRxiv - Biochemistry 2023Quote: ... and GoTaq 1-Step RT-qPCR System (Promega) were used for qPCR assays ...
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bioRxiv - Microbiology 2023Quote: ... 1 unit GoTaq G2 Flexi DNA Polymerase (Promega), 0.5 μM of each primer and 5 μL of total genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... 1 unit GoTaq G2 Flexi DNA Polymerase (Promega), 0.5 μM of each primer ...
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bioRxiv - Microbiology 2023Quote: ... 1 unit GoTaq G2 Flexi DNA Polymerase (Promega), 0.5 μM of each primer and 10 μL of DNA template ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-mouse IgG (Promega, W4021, 1:5000) and developed using enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2023Quote: ... containing 1 µg of sequencing-grade trypsin (Promega). Samples were digested overnight at 37°C and then subjected to an additional round of propionylation via the addition of 100 µL propionic anhydride solution (1:3 v/v isopropanol ...
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bioRxiv - Biochemistry 2023Quote: ... anti-mouse-IgG-HRP (W402B, 1:8000, Promega); anti-mouse-IgM-HRP (SC2064 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μl RNAsin Plus Inhibitor (Promega #N2615) were dissolved with water in 14 μl total volume ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing-grade trypsin (Promega, 1:25 enzyme:protein ratio) diluted in 20 µL digestion buffer (50 mM TEAB ...
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bioRxiv - Immunology 2024Quote: ... permeabilized with 1% TRITON X-100/PBS (Promega) and blocked with 5% FBS/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing-grade trypsin (Promega, 1:25 enzyme:protein ratio) diluted in 20 µl digestion buffer (50 mM TEAB ...
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bioRxiv - Biochemistry 2024Quote: ... 1:100 Endurazine Luciferase live cell substrate (Promega) were added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-Halo (1:100; G9211, Promega). Donkey secondary antibodies conjugated to Alexa Fluor 405/488/568/647 (Jackson ImmunoResearch or Thermo Fisher ...
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bioRxiv - Bioengineering 2024Quote: ... were: β-tubulin (1:1500, TUJ1, Promega, G7121), sox10 (1:300 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl of RNasinPlus 40 U/μl (Promega), 1μl RNA ligase 10 000 U/μl (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of cells was then processed using the Promega Wizard® genomic DNA preparation kit (Promega, WI, USA) to obtain genomic DNA ...
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bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: ... using a reaction solution consisting of RNA (100 or 200 ng) supplemented with 1 μL random primers (50 ng μL−1, Promega, C1181), 1 μL dNTPs (10 mM ...
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bioRxiv - Biophysics 2020Quote: IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Biophysics 2021Quote: ... IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-T-Cofilin 1:2000 (Bamburg lab, 1439 or Cell Signalling, 5175) and mouse anti-β3-tubulin 1:2000 (Promega, G7121) diluted in 1%BSA/PBS and incubated overnight at 4ºC ...
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bioRxiv - Cancer Biology 2022Quote: ... and the appropriate protein amount (60 μg for baseline expression experiments and 1mg for perturbation experiments) was digested with LysC (1:100, Wako, #121-05063) for 4 h and Trypsin (1:75, Promega, #V5113) overnight ...
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bioRxiv - Microbiology 2023Quote: The assay was adapted from 15 where Hyp1-Nluc schizonts at 1% hematocrit and 1-2% parasitaemia were lysed in 1x NanoGlo buffer (Promega, USA) within a 96-well flat-bottom plate and parasite lysates were added to compounds and incubated for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and RSPO1-CM were produced as described (Fenderico et al., 2019; Tauriello et al., 2010) Transfections were performed using either FuGENE 6 (Promega) according to manufacturer’s protocol for β-catenin-mediated reporter assays and microscopy or polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...